Col4a1 (NM_009931) Mouse Tagged ORF Clone
CAT#: MR222881
- TrueORF®
Col4a1 (Myc-DDK-tagged) - Mouse collagen, type IV, alpha 1 (Col4a1)
"NM_009931" in other vectors (2)
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Col4a1 |
Synonyms | Bru; Col4a-1; Raw; Svc |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR222881 representing NM_009931
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCCCCGGCTCAGCGTCTGGCTTCTGCTGCTCTTCGCCGCCCTTCTGCTCCACGAGGAGCGCAGCC GAGCAGCTGCGAAGGGCGATTGTGGTGGCTCTGGCTGTGGAAAATGTGACTGTCATGGCGTGAAGGGACA AAAGGGAGAAAGAGGCTTGCCAGGGTTGCAAGGTGTCATTGGATTTCCCGGGATGCAAGGACCTGAGGGG CCACATGGACCACCAGGACAAAAGGGTGATGCTGGAGAACCTGGACTTCCTGGCACAAAAGGGACGAGGG GACCCCCTGGAGCAGCTGGCTACCCTGGGAATCCCGGACTTCCTGGTATTCCTGGCCAAGATGGCCCTCC AGGTCCCCCAGGTATCCCAGGATGCAACGGTACAAAGGGAGAGAGAGGGCCGCTCGGTCCTCCTGGCTTG CCTGGATTCAGCGGCAATCCTGGACCACCAGGGTTACCAGGAATGAAGGGAGATCCTGGTGAAATTCTCG GCCACGTTCCCGGAACACTGCTGAAAGGGGAGAGAGGATTTCCTGGTATCCCCGGGATGCCGGGCTCACC AGGGTTGCCAGGACTCCAGGGACCCGTGGGTCCTCCGGGATTTACTGGACCACCGGGTCCTCCAGGCCCT CCTGGACCTCCTGGAGAAAAGGGCCAGATGGGCTCCAGCTTCCAAGGACCAAAAGGTGACAAGGGAGAGC AAGGGGTCAGCGGTCCCCCGGGAGTTCCTGGACAGGCACAAGTTAAGGAAAAAGGAGACTTTGCCCCAAC AGGAGAAAAGGGTCAGAAAGGTGAACCTGGATTTCCGGGAGTACCAGGATATGGAGAGAAAGGTGAACCT GGCAAGCAAGGGCCTCGGGGAAAACCTGGGAAAGACGGTGAAAAAGGAGAAAGGGGGAGCCCGGGCATTC CTGGCGATTCCGGGTACCCAGGTCTCCCAGGCCGGCAGGGCCCGCAGGGAGAGAAGGGTGAAGCTGGACT TCCAGGCCCCCCTGGAACTGTGATAGGCACCATGCCTTTGGGAGAGAAAGGAGATCGAGGCTACCCAGGA GCACCAGGGTTGAGAGGAGAGCCCGGCCCTAAAGGTTTCCCTGGAACACCAGGCCAACCAGGCCCTCCAG GTTTCCCTACTCCAGGCCAGGCTGGTGCTCCAGGCTTCCCAGGTGAAAGGGGAGAAAAAGGTGACCAGGG ATTTCCAGGCGTGTCATTGCCAGGACCAAGTGGAAGAGATGGAGCCCCGGGGCCTCCCGGCCCTCCCGGC CCCCCTGGGCAGCCAGGCCACACAAATGGCATTGTGGAGTGTCAACCTGGACCACCCGGGGACCAGGGCC CTCCTGGGACTCCAGGACAGCCAGGTTTGACAGGTGAAGTTGGACAGAAAGGTCAGAAAGGAGAGAGCTG CCTTGCCTGTGACACAGAAGGACTTCGTGGCCCTCCAGGGCCACAGGGGCCTCCGGGAGAGATTGGTTTC CCTGGACAACCTGGGGCTAAAGGTGACCGAGGCCTGCCCGGCAGAGATGGTCTTGAAGGATTGCCGGGTC CACAAGGTTCACCAGGGCTTATAGGCCAGCCTGGAGCTAAGGGAGAGCCTGGAGAGATATTTTTTGACAT GCGACTCAAAGGTGACAAAGGAGACCCAGGTTTTCCGGGACAGCCTGGCATGCCAGGAAGAGCAGGAACT CCCGGAAGAGATGGCCACCCAGGACTCCCTGGACCCAAAGGCTCTCCGGGTTCAATAGGATTGAAAGGAG AGCGAGGTCCTCCCGGAGGAGTTGGCTTCCCTGGTAGTCGTGGAGACATCGGCCCTCCTGGACCCCCAGG AGTTGGCCCCATTGGTCCTGTTGGTGAAAAAGGACAAGCAGGCTTTCCTGGAGGCCCTGGGTCCCCAGGT CTTCCAGGCCCAAAGGGTGAGGCAGGAAAGGTTGTCCCACTCCCTGGCCCCCCAGGAGCTGCAGGACTTC CAGGATCCCCTGGCTTCCCAGGGCCACAAGGTGACCGAGGCTTCCCAGGAACCCCAGGACGTCCAGGCAT CCCGGGAGAGAAAGGTGCTGTGGGCCAGCCAGGAATTGGATTTCCTGGGCTTCCTGGCCCCAAAGGTGTT GATGGCTTGCCTGGAGAGATAGGACGGCCTGGGAGTCCAGGTCGCCCTGGATTTAACGGCTTACCTGGCA ACCCAGGACCTCAAGGTCAAAAGGGAGAACCTGGCATTGGGCTTCCAGGACTCAAAGGGCAACCAGGCCT TCCAGGCATTCCCGGTACACCTGGAGAGAAGGGTAGCATCGGGGGACCCGGCGTTCCAGGAGAACAGGGG TTGACAGGCCCCCCAGGACTCCAGGGGATCAGAGGTGACCCAGGGCCTCCTGGAGTTCAAGGCCCAGCAG GTCCACCAGGGGTCCCAGGAATAGGGCCACCCGGAGCTATGGGCCCTCCCGGAGGGCAAGGACCACCAGG GTCATCAGGTCCACCTGGAATTAAAGGAGAGAAAGGGTTCCCTGGATTCCCCGGACTGGATATGCCTGGC CCCAAAGGCGATAAAGGCTCTCAAGGACTTCCTGGCCTCACAGGACAGTCAGGCCTCCCTGGCCTTCCTG GACAGCAGGGGACACCTGGAGTTCCAGGGTTCCCAGGTTCTAAAGGTGAAATGGGTGTCATGGGAACCCC GGGACAACCAGGCTCGCCAGGACCAGCAGGCACCCCAGGGTTACCTGGAGAAAAAGGGGACCATGGCCTT CCGGGCTCCTCAGGACCCAGGGGCGACCCTGGCTTCAAAGGTGATAAAGGTGACGTTGGGCTTCCTGGCA TGCCAGGATCCATGGAGCATGTGGACATGGGGAGCATGAAGGGACAGAAAGGAGACCAGGGAGAGAAAGG ACAAATCGGACCCACTGGTGATAAAGGTTCCCGAGGAGACCCTGGAACACCAGGAGTACCTGGGAAGGAT GGGCAGGCAGGGCATCCCGGACAGCCAGGGCCTAAAGGTGACCCAGGCCTTAGTGGGACACCAGGATCCC CTGGACTCCCTGGACCCAAAGGATCAGTTGGAGGAATGGGCTTGCCAGGTTCGCCTGGAGAAAAAGGTGT GCCTGGCATCCCTGGCTCACAGGGTGTCCCTGGCTCACCTGGAGAGAAGGGAGCCAAAGGAGAGAAAGGG CAGTCAGGTCTACCTGGCATTGGGATTCCGGGACGGCCTGGTGACAAGGGAGACCAGGGCCTTGCTGGCT TCCCAGGCAGCCCCGGTGAGAAGGGAGAGAAAGGCAGTGCCGGAACCCCAGGGATGCCAGGGTCCCCAGG CCCGAGAGGTTCTCCGGGGAACATCGGCCATCCAGGAAGCCCAGGCCTGCCTGGAGAGAAAGGGGATAAA GGCCTCCCAGGACTGGATGGCGTTCCTGGTGTCAAAGGAGAAGCAGGTCTCCCTGGGACTCCTGGCCCCA CAGGCCCAGCTGGCCAGAAGGGAGAGCCGGGCAGCGATGGAATCCCGGGGTCGGCAGGAGAGAAGGGTGA ACAAGGTGTTCCAGGAAGAGGCTTCCCCGGCTTCCCTGGCTCCAAAGGAGACAAAGGCTCCAAGGGTGAA GTGGGTTTCCCTGGCCTAGCTGGAAGTCCTGGAATTCCTGGAGTCAAAGGCGAGCAAGGGTTCATGGGTC CTCCTGGCCCTCAAGGACAACCTGGCTTACCTGGCACTCCTGGTCACCCTGTGGAGGGGCCCAAAGGAGA CCGAGGACCTCAGGGTCAACCTGGCCTGCCAGGGCATCCGGGACCTATGGGGCCGCCAGGGTTCCCAGGA ATCAATGGGCCAAAAGGTGACAAGGGAAATCAAGGTTGGCCAGGAGCTCCGGGGGTTCCAGGCCCTAAGG GAGACCCAGGATTCCAAGGCATGCCGGGCATTGGCGGCTCTCCAGGGATCACAGGTTCAAAGGGAGATAT GGGACTGCCCGGAGTTCCAGGATTTCAAGGTCAGAAAGGGCTTCCTGGTCTGCAGGGAGTGAAAGGAGAC CAGGGAGACCAAGGTGTACCCGGCCCTAAAGGTCTCCAAGGTCCCCCTGGGCCTCCAGGTCCCTACGATG TCATCAAAGGAGAACCAGGGCTCCCTGGTCCTGAGGGTCCCCCTGGTCTTAAAGGACTCCAGGGACCACC AGGTCCAAAAGGACAGCAAGGTGTGACAGGCTCAGTGGGCTTGCCTGGACCTCCAGGTGTCCCTGGGTTC GATGGTGCTCCTGGCCAGAAAGGAGAGACAGGACCCTTTGGACCACCTGGTCCAAGAGGGTTTCCTGGCC CACCAGGCCCCGATGGGCTGCCAGGATCCATGGGTCCCCCAGGTACCCCATCTGTGGACCATGGCTTCCT TGTGACCAGGCATAGTCAGACAACAGATGACCCACTGTGTCCCCCAGGGACCAAAATTCTTTACCATGGA TACTCTCTGCTCTATGTCCAAGGCAACGAGCGTGCCCACGGGCAGGACTTGGGTACGGCTGGCAGCTGCC TGCGTAAGTTCAGCACCATGCCCTTTCTCTTCTGCAACATCAACAACGTCTGCAACTTCGCCTCCAGGAA CGACTACTCTTACTGGCTGTCCACGCCAGAGCCCATGCCCATGTCCATGGCACCCATCTCTGGGGACAAC ATCCGGCCCTTCATTAGCAGGTGTGCGGTTTGTGAAGCACCGGCCATGGTGATGGCGGTACACAGTCAGA CCATTCAGATTCCGCAGTGCCCTAACGGTTGGTCCTCACTGTGGATCGGCTATTCCTTCGTGATGCACAC CAGCGCTGGTGCTGAAGGTTCCGGCCAAGCCCTCGCATCCCCCGGGTCCTGTCTGGAAGAGTTTAGAAGC GCCCCATTCATCGAGTGCCACGGCAGAGGAACGTGCAATTACTACGCAAATGCTTACAGCTTTTGGCTCG CCACCATAGAGAGAAGCGAGATGTTCAAGAAGCCCACGCCATCCACCTTGAAGGCAGGGGAGCTGCGAAC ACACGTCAGCCGCTGCCAAGTGTGCATGAGAAGAACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222881 representing NM_009931
Red=Cloning site Green=Tags(s) MGPRLSVWLLLLFAALLLHEERSRAAAKGDCGGSGCGKCDCHGVKGQKGERGLPGLQGVIGFPGMQGPEG PHGPPGQKGDAGEPGLPGTKGTRGPPGAAGYPGNPGLPGIPGQDGPPGPPGIPGCNGTKGERGPLGPPGL PGFSGNPGPPGLPGMKGDPGEILGHVPGTLLKGERGFPGIPGMPGSPGLPGLQGPVGPPGFTGPPGPPGP PGPPGEKGQMGSSFQGPKGDKGEQGVSGPPGVPGQAQVKEKGDFAPTGEKGQKGEPGFPGVPGYGEKGEP GKQGPRGKPGKDGEKGERGSPGIPGDSGYPGLPGRQGPQGEKGEAGLPGPPGTVIGTMPLGEKGDRGYPG APGLRGEPGPKGFPGTPGQPGPPGFPTPGQAGAPGFPGERGEKGDQGFPGVSLPGPSGRDGAPGPPGPPG PPGQPGHTNGIVECQPGPPGDQGPPGTPGQPGLTGEVGQKGQKGESCLACDTEGLRGPPGPQGPPGEIGF PGQPGAKGDRGLPGRDGLEGLPGPQGSPGLIGQPGAKGEPGEIFFDMRLKGDKGDPGFPGQPGMPGRAGT PGRDGHPGLPGPKGSPGSIGLKGERGPPGGVGFPGSRGDIGPPGPPGVGPIGPVGEKGQAGFPGGPGSPG LPGPKGEAGKVVPLPGPPGAAGLPGSPGFPGPQGDRGFPGTPGRPGIPGEKGAVGQPGIGFPGLPGPKGV DGLPGEIGRPGSPGRPGFNGLPGNPGPQGQKGEPGIGLPGLKGQPGLPGIPGTPGEKGSIGGPGVPGEQG LTGPPGLQGIRGDPGPPGVQGPAGPPGVPGIGPPGAMGPPGGQGPPGSSGPPGIKGEKGFPGFPGLDMPG PKGDKGSQGLPGLTGQSGLPGLPGQQGTPGVPGFPGSKGEMGVMGTPGQPGSPGPAGTPGLPGEKGDHGL PGSSGPRGDPGFKGDKGDVGLPGMPGSMEHVDMGSMKGQKGDQGEKGQIGPTGDKGSRGDPGTPGVPGKD GQAGHPGQPGPKGDPGLSGTPGSPGLPGPKGSVGGMGLPGSPGEKGVPGIPGSQGVPGSPGEKGAKGEKG QSGLPGIGIPGRPGDKGDQGLAGFPGSPGEKGEKGSAGTPGMPGSPGPRGSPGNIGHPGSPGLPGEKGDK GLPGLDGVPGVKGEAGLPGTPGPTGPAGQKGEPGSDGIPGSAGEKGEQGVPGRGFPGFPGSKGDKGSKGE VGFPGLAGSPGIPGVKGEQGFMGPPGPQGQPGLPGTPGHPVEGPKGDRGPQGQPGLPGHPGPMGPPGFPG INGPKGDKGNQGWPGAPGVPGPKGDPGFQGMPGIGGSPGITGSKGDMGLPGVPGFQGQKGLPGLQGVKGD QGDQGVPGPKGLQGPPGPPGPYDVIKGEPGLPGPEGPPGLKGLQGPPGPKGQQGVTGSVGLPGPPGVPGF DGAPGQKGETGPFGPPGPRGFPGPPGPDGLPGSMGPPGTPSVDHGFLVTRHSQTTDDPLCPPGTKILYHG YSLLYVQGNERAHGQDLGTAGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPISGDN IRPFISRCAVCEAPAMVMAVHSQTIQIPQCPNGWSSLWIGYSFVMHTSAGAEGSGQALASPGSCLEEFRS APFIECHGRGTCNYYANAYSFWLATIERSEMFKKPTPSTLKAGELRTHVSRCQVCMRRT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009931 |
ORF Size | 5007 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_009931.1, NM_009931.2, NP_034061.2 |
RefSeq Size | 6636 |
RefSeq ORF | 5010 |
Locus ID | 12826 |
MW | 160.7 kDa |
Gene Summary | This gene encodes the alpha-1 subunit of the type IV collagens, an essential component of basement membranes. The encoded protein forms a triple helical heterotrimer comprised of two alpha-1 and one alpha-2 subunits that assembles into a type IV collagen network. This gene is located adjacent to the gene encoding alpha-2 subunit. Mice lacking both the alpha-1 and alpha-2 subunits of collagen IV die in utero due to structural deficiencies in the basement membranes and certain mutations in this gene cause perinatal cerebral hemorrhage and porencephaly. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015] |
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