Lig1 (NM_010715) Mouse Tagged ORF Clone

CAT#: MR222972

Lig1 (Myc-DDK-tagged) - Mouse ligase I, DNA, ATP-dependent (Lig1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lig1
• Synonyms: AL033288; LigI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR222972 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGAAGTATTATGTCATTTTTTCAACCCACAAAAGAAGGTAAAGCGAAGAAGCCAGAGAAGGAGA
  CACCCAGCAGCATCAGAGAGAAGGAACCCCCTCCAAAGGTGGCGCTGAAGGAGAGGAATCAAGTGGTGCC
  CGAGAGTGATTCTCCAGTGAAGAGGACAGGAAGGAAGGTAGCCCAGGTTCTGAGCTGTGAAGGGGAGGAC
  GAAGATGAAGCCCCTGGCACCCCCAAAGTCCAGAAGCCTGTGTCAGACTCTGAACAGAGCTCTCCTCCCA
  GCCCTGACACATGTCCTGAGAACAGTCCTGTCTTCAACTGCAGCTCCCCCATGGACATCTCCCCATCAGG
  ATTCCCAAAGCGTCGAACTGCGCGGAAGCAGCTCCCCAAACGGACAATTCAAGACACTCTTGAGGAGCAG
  AATGAGGACAAAACCAAAACAGCTAAGAAAAGGAAGAAAGAAGAAGAGACCCCAAAAGAAAGCCTCGCAG
  AGGCTGAAGACGTAAAACAGAAGGAAGAAAAGGAGGGGGACCAGCTCATAGTCCCCTCTGAGCCCACAAA
  GTCCCCTGAGTCAGTAACCCTGACAAAGACAGAGAACATTCCAGTGTGTAAGGCAGGAGTGAAACTGAAG
  CCTCAGGAAGAGGAACAGAGCAAACCTCCTGCTAGAGGTGCCAAGACACTGAGCAGTTTCTTCACACCCC
  GAAAACCAGCAGTGAAAACTGAAGTCAAACAAGAAGAGTCAGGCACTCTAAGGAAGGAAGAGACCAAGGG
  AACCCTGGATCCAGCAAATTATAATCCTTCCAAGAATAACTATCATCCCATTGAAGATGCCTGCTGGAAA
  CATGGCCAGAAGGTCCCTTTTCTCGCCGTGGCCCGGACATTTGAGAAGATTGAGGAGGTTTCTGCTAGGC
  TCAAGATGGTGGAGACATTGAGCAACCTGCTGCGCTCCGTGGTGGCCCTGTCCCCTCCAGACTTGCTCCC
  TGTTCTCTATCTCAGCCTCAACCGCCTCGGGCCACCTCAGCAGGGACTAGAGCTGGGTGTTGGTGATGGT
  GTCCTCCTCAAGGCCGTTGCCCAGGCCACAGGTCGTCAGCTGGAGTCCATCCGGGCTGAGGTAGCTGAGA
  AGGGGGATGTGGGGCTGGTGGCCGAGAACAGCCGCAGCACTCAGAGACTCATGCTGCCTCCACCTCCACT
  TACTATTTCTGGAGTCTTCACCAAATTCTGTGACATTGCCCGGCTCACTGGCAGTGCTTCCATGGCCAAG
  AAGATGGACATTATCAAGGGCCTTTTTGTTGCCTGCCGCCACTCAGAAGCCAGGTACATTGCCAGGTCCC
  TAAGCGGGCGGCTGCGCCTTGGCCTAGCTGAGCAGTCGGTACTGGCTGCTCTTGCCCAGGCTGTGAGCCT
  CACACCCCCTGGCCAAGAATTTCCCACGGCGGTGGTGGATGCTGGGAAGGGCAAGACAGCAGAGGCCAGA
  AAGATGTGGTTGGAAGAACAAGGCATGATCTTGAAGCAGACCTTCTGTGAGGTTCCTGACCTGGATCGAA
  TCATCCCTGTGCTGCTGGAACATGGCCTGGAACGTCTCCCAGAGCACTGCAAGCTGAGCCCAGGGGTCCC
  TCTTAAACCAATGCTGGCTCATCCCACTCGGGGTGTCAGTGAGGTCCTAAAACGCTTTGAGGAGGTCGAC
  TTCACCTGTGAATATAAATACGATGGGCAGCGAGCACAGATACATGTTCTGGAAGGTGGAGAAGTGAAGA
  TCTTCAGCAGGAACCAGGAAGACAACACAGGAAAGTATCCTGACATTATCAGCCGCATCCCCAAGATTAA
  ACACCCCTCAGTCACATCTTTCATTCTGGACACTGAGGCTGTGGCCTGGGACCGGGAAAAGAAGCAGATC
  CAACCATTCCAAGTACTCACCACGCGCAAGCGCAAGGAGGTTGATGCATCGGAGATACAGGTGCAAGTGT
  GTCTGTATGCTTTTGACCTCATCTACCTCAACGGAGAGTCCCTGGTTCGCCAGCCCCTGTCTCGACGCCG
  GCAGCTGCTCCGTGAGAACTTCGTGGAGACAGAGGGTGAGTTTGTCTTCACCACCTCTTTGGACACCAAG
  GACACCGAGCAGATTGCTGAGTTCTTGGAGCAGTCAGTGAAGGACTCCTGTGAGGGCCTGATGGTGAAGA
  CCTTGGATGTTGATGCCACTTATGAGATTGCCAAGAGGTCACACAACTGGCTCAAGCTGAAGAAGGACTA
  CCTTGACGGTGTGGGTGACACTCTGGACCTTGTGGTGATTGGTGCCTACCTGGGCCGGGGGAAGCGGGCT
  GGCCGGTATGGAGGCTTCCTGTTGGCTGCCTACGATGAGGAGAGTGAAGAGCTTCAGGCTATATGCAAGC
  TGGGAACTGGATTCAGCGATGAGGAGCTGGAGGAACACCACCAGAGCCTGCAGGCCCTGGTATTGCCTAC
  CCCACGCCCCTATGTGAGGATTGATGGGGCAGTGGCCCCAGACCATTGGCTGGACCCAAGCATTGTGTGG
  GAGGTGAAGTGTGCTGATCTTTCCCTGTCCCCCATCTACCCTGCTGCACGGGGCCTGGTGGACAAAGAAA
  AAGGGATCTCTCTTCGTTTTCCTCGGTTCATTCGTGTCCGTAAAGACAAGCAGCCAGAGCAGGCCACTAC
  CAGTAACCAGGTTGCCTCTTTGTACCGGAAGCAGAGTCAGATTCAGAACCAACAAAGCTCAGACTTGGAC
  TCCGACGTTGAAGACTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR222972 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQRSIMSFFQPTKEGKAKKPEKETPSSIREKEPPPKVALKERNQVVPESDSPVKRTGRKVAQVLSCEGED
  EDEAPGTPKVQKPVSDSEQSSPPSPDTCPENSPVFNCSSPMDISPSGFPKRRTARKQLPKRTIQDTLEEQ
  NEDKTKTAKKRKKEEETPKESLAEAEDVKQKEEKEGDQLIVPSEPTKSPESVTLTKTENIPVCKAGVKLK
  PQEEEQSKPPARGAKTLSSFFTPRKPAVKTEVKQEESGTLRKEETKGTLDPANYNPSKNNYHPIEDACWK
  HGQKVPFLAVARTFEKIEEVSARLKMVETLSNLLRSVVALSPPDLLPVLYLSLNRLGPPQQGLELGVGDG
  VLLKAVAQATGRQLESIRAEVAEKGDVGLVAENSRSTQRLMLPPPPLTISGVFTKFCDIARLTGSASMAK
  KMDIIKGLFVACRHSEARYIARSLSGRLRLGLAEQSVLAALAQAVSLTPPGQEFPTAVVDAGKGKTAEAR
  KMWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGVPLKPMLAHPTRGVSEVLKRFEEVD
  FTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGKYPDIISRIPKIKHPSVTSFILDTEAVAWDREKKQI
  QPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVRQPLSRRRQLLRENFVETEGEFVFTTSLDTK
  DTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRA
  GRYGGFLLAAYDEESEELQAICKLGTGFSDEELEEHHQSLQALVLPTPRPYVRIDGAVAPDHWLDPSIVW
  EVKCADLSLSPIYPAARGLVDKEKGISLRFPRFIRVRKDKQPEQATTSNQVASLYRKQSQIQNQQSSDLD
  SDVEDY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010715
• ORF Size: 2751 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010715.1
• RefSeq Size: 3111 bp
• RefSeq ORF: 2799 bp
• Locus ID: 16881
• MW: 102.2 kDa
• Gene Summary: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. [UniProtKB/Swiss-Prot Function]