Braf (NM_139294) Mouse Tagged ORF Clone

CAT#: MR223023

Braf (Myc-DDK-tagged) - Mouse Braf transforming gene (Braf)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Braf
• Synonyms: 9930012E13Rik; AA120551; AA387315; AA473386; B-raf; Braf-2; Braf2; C87398; C230098H17; D6Ertd631e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223023 representing NM_139294
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCTGAGTGGCGGCGGTGGCAGCAGCAGCGGTGGCGGCGGCGGCGGTGGCGGCGGCGGTGGCG
  GTGGCGACGGCGGCGGCGGCGCCGAGCAGGGCCAGGCTCTGTTCAATGGCGACATGGAGCCGGAGGCCGG
  CGCTGGCGCCGCGGCCTCTTCGGCTGCGGACCCGGCCATTCCTGAAGAGGTATGGAATATCAAGCAAATG
  ATTAAGTTGACACAGGAACATATAGAGGCCCTATTGGACAAATTTGGTGGAGAGCATAACCCACCATCAA
  TATACCTGGAGGCCTATGAAGAGTACACCAGCAAGCTAGATGCCCTTCAGCAAAGAGAACAGCAGCTTTT
  GGAATCCCTGGTTTTTCAAACTCCCACAGATGCATCACGGAACAACCCCAAGTCACCACAGAAACCTATC
  GTTAGAGTCTTCCTGCCCAACAAACAGAGGACAGTGGTACCCGCAAGATGTGGTGTTACAGTTCGAGACA
  GTCTAAAGAAAGCACTGATGATGAGAGGTCTCATCCCAGAATGCTGTGCTGTTTACAGAATTCAGGATGG
  AGAGAAGAAACCAATTGGCTGGGACACGGACATTTCCTGGCTTACTGGAGAGGAGTTACATGTTGAAGTA
  CTGGAGAATGTCCCACTTACAACACACAACTTTGTACGGAAAACTTTTTTCACCTTAGCATTTTGTGACT
  TTTGCCGAAAGCTGCTTTTCCAGGGTTTCCGTTGTCAAACATGTGGTTATAAATTTCACCAGCGTTGTAG
  TACAGAGGTTCCACTGATGTGTGTAAATTATGACCAACTTGATTTGCTGTTTGTCTCCAAGTTCTTTGAG
  CATCACCCAGTACCACAGGAGGAGGCCTCCTTCCCAGAGACTGCCCTTCCATCTGGATCCTCTTCCGCAC
  CCCCCTCAGACTCTACTGGGCCCCAAATCCTCACCAGTCCATCTCCTTCAAAATCCATTCCAATTCCACA
  GCCCTTCCGACCAGCAGATGAAGATCATCGCAATCAGTTTGGGCAACGAGACCGGTCCTCCTCAGCTCCC
  AATGTTCATATAAACACAATTGAGCCTGTGAATATCGATGAAAAATTCCCAGAAGTGGAATTACAGGATC
  AAAGGGATTTGATTAGAGACCAGGGGTTTCGTGGTGATGGAGCCCCCTTGAACCAACTGATGCGCTGTCT
  TCGGAAATACCAATCCCGGACTCCCAGCCCCCTCCTCCATTCTGTCCCCAGTGAAATAGTGTTTGATTTT
  GAGCCTGGCCCAGTGTTCAGAGGGTCAACCACAGGCTTGTCCGCCACCCCGCCTGCCTCATTACCTGGCT
  CACTCACTAACGTGAAAGCCTTACAGAAATCTCCAGGTCCTCAGCGGGAAAGGAAGTCATCTTCTTCCTC
  ATCCTCGGAGGACAGAAGTCGGATGAAAACACTTGGTAGAAGAGATTCAAGTGATGACTGGGAGATTCCT
  GATGGACAGATTACAGTGGGACAGAGAATTGGATCTGGGTCATTTGGAACTGTCTACAAGGGAAAGTGGC
  ATGGTGATGTGGCAGTGAAAATGTTGAATGTGACAGCACCCACACCTCAACAGCTACAGGCCTTCAAAAA
  TGAAGTAGGAGTGCTCAGGAAAACTCGACATGTGAATATCCTCCTTTTCATGGGCTATTCTACAAAGCCA
  CAACTGGCAATTGTTACACAGTGGTGTGAGGGCTCCAGCTTATATCACCATCTCCACATCATTGAGACCA
  AATTTGAGATGATCAAACTTATAGATATTGCTCGGCAGACTGCACAGGGCATGGATTACTTACACGCCAA
  GTCAATCATCCACAGAGACCTCAAGAGTAATAATATATTTCTTCATGAAGACCTCACGGTAAAAATAGGT
  GACTTTGGTCTAGCCACAGTGAAATCTCGGTGGAGTGGGTCCCATCAGTTTGAACAGTTGTCTGGATCTA
  TTTTGTGGATGGCACCAGAAGTAATCAGAATGCAAGATAAAAACCCGTATAGCTTTCAGTCAGACGTGTA
  TGCGTTTGGGATTGTTCTGTACGAACTGATGACCGGCCAGCTACCTTATTCAAACATCAACAACAGGGAT
  CAGATAATTTTTATGGTGGGACGAGGATACCTATCTCCAGATCTCAGTAAGGTACGGAGTAACTGTCCAA
  AAGCCATGAAGAGATTAATGGCAGAGTGCCTCAAAAAGAAAAGAGACGAGAGACCACTCTTTCCCCAAAT
  TCTCGCCTCCATTGAGCTGCTGGCCCGCTCATTGCCAAAAATTCACCGCAGTGCATCAGAACCTTCCTTG
  AATCGGGCTGGTTTCCAAACAGAAGATTTTAGTCTGTATGCTTGTGCTTCTCCGAAAACACCCATCCAAG
  CAGGGGGATATGGAGAATTTGCAGCCTTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223023 representing NM_139294
  Red=Cloning site Green=Tags(s)
  
  MAALSGGGGSSSGGGGGGGGGGGGGDGGGGAEQGQALFNGDMEPEAGAGAAASSAADPAIPEEVWNIKQM
  IKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLVFQTPTDASRNNPKSPQKPI
  VRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEV
  LENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFE
  HHPVPQEEASFPETALPSGSSSAPPSDSTGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAP
  NVHINTIEPVNIDEKFPEVELQDQRDLIRDQGFRGDGAPLNQLMRCLRKYQSRTPSPLLHSVPSEIVFDF
  EPGPVFRGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSSSEDRSRMKTLGRRDSSDDWEIP
  DGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKP
  QLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIG
  DFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRD
  QIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSL
  NRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139294
• ORF Size: 2412 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_139294.3, NM_139294.4, NM_139294.5, NP_647455.3
• RefSeq Size: 9728
• RefSeq ORF: 2415
• Locus ID: 109880
• MW: 89.2 kDa