Cacna1s (NM_014193) Mouse Tagged ORF Clone

CAT#: MR223026

Cacna1s (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, L type, alpha 1S subunit (Cacna1s), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cacna1s
• Synonyms: AW493108; Cav1.1; Cchl1a3; DHPR; DHPR alpha1s; fmd; mdg; sj
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223026 representing NM_014193
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGCCCTCACCCCAGGACGAGGGCCTGAGGAAGAAGCAGCCGAAGAAACCTGTTCCTGAGATTC
  TGCCGAGGCCCCCGCGGGCCTTGTTCTGCCTGACTCTACAGAACCCCCTGAGGAAGGCCTGCATCAGCAT
  CGTGGAATGGAAACCCTTCGAGACCATCATCTTGCTCACCATCTTTGCCAACTGTGTGGCCCTGGCAGTG
  TACCTGCCCATGCCAGAGGATGACAACAACACTCTGAACCTCGGCCTGGAGAAACTGGAGTACTTCTTCC
  TCATTGTCTTCTCCATTGAAGCCGCCATGAAGATCATAGCCTACGGCTTCCTATTCCATCAGGATGCATA
  CCTGCGCAGTGGCTGGAACGTTCTGGACTTCATCATTGTCTTTCTGGGGGTCTTCACTGTGATTCTGGAA
  CAAGTTAACATCATTCAAACCAACACGGCCCCCATGAGCAGCAAAGGAGCCGGCTTGGATGTGAAGGCCC
  TTCGAGCATTCCGCGTGCTCAGACCCCTGCGGCTGGTGTCGGGGGTGCCCAGTCTTCAGGTGGTCCTCAA
  CTCCATCTTCAAGGCCATGCTCCCCCTGTTCCACATTGCCCTGCTCGTCCTCTTCATGGTCATCATCTAC
  GCCATCATTGGGCTGGAGCTCTTCAAAGGCAAGATGCACAAGACTTGCTACTTCATTGGGACAGACATTG
  TAGCCACAGTTGAGAATGAGAAACCATCACCCTGTGCTAGAACTGGCTCTGGGCGTCCCTGCACCATCAA
  TGGCAGCGAGTGCCGAGGAGGTTGGCCAGGACCCAACCACGGCATCACCCACTTCGACAACTTTGGCTTC
  TCTATGCTCACTGTGTATCAATGCATCTCCATGGAGGGGTGGACTGATGTCCTGTACTGGGTCAACGATG
  CCATCGGGAATGAGTGGCCCTGGATCTACTTTGTCACCCTCATTCTGCTGGGCTCCTTCTTCATCCTCAA
  CCTGGTGCTGGGTGTCCTGAGTGGAGAATTCACCAAAGAGCGAGAGAAGGCCAAGTCCAGGGGAACCTTC
  CAAAAGCTCCGAGAGAAACAGCAGCTGGAGGAGGACCTCCGTGGTTACATGAGCTGGATCACACAGGGCG
  AGGTCATGGACGTGGACGACTTGAGAGAAGGCAAGCTGTCTTTGGATGAAGGGGGATCAGACACAGAGAG
  CTTGTATGAAATCGAAGGCTTGAACAAAATCATCCAGTTCATTCGGCACTGGCGACAGTGGAATCGCGTC
  TTCCGCTGGAAGTGCCATGACCTAGTGAAATCCAAGGTCTTCTATTGGCTGGTGATCCTGATCGTTGCCC
  TAAACACCCTGTCCATTGCCTCGGAGCACCACAACCAGCCACTCTGGTTGACCCATTTGCAAGATGTTGC
  CAACCGCGTGTTGCTGACCCTCTTCACCATCGAAATGCTCATGAAGATGTACGGGCTGGGCCTGCGCCAG
  TACTTCATGTCCATCTTCAACCGCTTCGACTGCTTCGTGGTGTGCAGCGGCATCCTGGAGATCCTGCTGG
  TGGAGTCGGGAGCCATGTCGCCCCTGGGCATCTCTGTGCTCCGCTGTATCCGCCTCCTGAGGCTCTTCAA
  GATCACCAAGTACTGGACATCGCTGAGCAACCTGGTGGCCTCACTGCTCAACTCCATCCGCTCCATTGCT
  TCCCTCTTGCTGCTGCTCTTTCTCTTCATCATCATCTTTGCCCTGCTGGGCATGCAGCTCTTTGGAGGCA
  GATATGACTTTGAAGACACAGAAGTACGACGAAGCAACTTTGACAACTTCCCCCAGGCCCTCATCAGTGT
  CTTCCAGGTCCTGACGGGTGAAGACTGGAACTCTGTGATGTACAATGGGATCATGGCTTACGGAGGTCCG
  ACCTACCCTGGTGTGCTGGTGTGCATTTATTTCATCATCCTTTTCGTCTGTGGCAACTACATCCTTCTCA
  ACGTCTTCCTGGCTATTGCTGTGGACAACCTGGCAGAGGCAGAGAGTCTGACATCTGCCCAAAAGGCCAA
  GGCTGAGGAGAGAAAGCGCAGGAAGATGTCGAAAGGGCTCCCAGACAAGTCAGAAGAGGAGAGGGCCACG
  GTGACCAAGAAACTGGAGCAGAAGTCCAAGGGTGAAGGCATCCCCACCACCGCCAAGCTGAAAATCGATG
  AGTTTGAATCAAACGTTAATGAGGTGAAAGACCCCTACCCTTCGGCTGACTTCCCAGGGGACGATGAGGA
  AGATGAGCCCGAGATCCCAGTGAGCCCCCGACCACGCCCTCTGGCCGAGCTGCAGCTGAAAGAGAAAGCA
  GTGCCCATCCCGGAGGCTAGCTCCTTCTTCATCTTCAGTCCCACCAACAAGATCCGTGTCCTGTGTCACC
  GCATTGTCAATGCCACCTGGTTCACCAACTTCATCCTGCTCTTCATCCTGCTCAGCAGTGCCGCCCTGGC
  CGCTGAGGATCCCATCCGGGCCGACTCCATGAGGAATCAGATCCTCGAGTATTTTGATTATGTGTTCACT
  GCTGTTTTCACGGTGGAGATTGTCCTTAAGATGACCACCTATGGAGCCTTCCTGCACAAAGGCTCCTTCT
  GCCGCAACTACTTCAACATCCTGGACCTGCTGGTGGTGGCTGTGTCCCTCATCTCCATGGGCCTCGAGTC
  CAGCGCCATCTCCGTGGTGAAGATCTTAAGGGTTCTGAGAGTGCTGAGACCACTCCGGGCCATAAACAGA
  GCCAAAGGGTTGAAGCACGTGGTGCAGTGCGTGTTTGTTGCCATTCGCACCATCGGGAACATCGTGCTGG
  TCACCACGCTCCTGCAGTTCATGTTTGCGTGCATTGGAGTGCAGCTTTTCAAGGGAAAGTTCTACAGCTG
  CAATGACTTATCCAAGATGACTGAAGAGGAATGCAGGGGCTACTACTACATATACAAGGATGGAGACCCC
  ACTCAGATAGAGCTGCGTCCCCGCCAGTGGATACACAATGACTTCCACTTTGACAATGTACTCTCCGCTA
  TGATGTCACTCTTCACGGTCTCCACCTTCGAGGGTTGGCCTCAGCTACTGTACAAGGCCATAGACTCAAA
  TGAGGAGGACACCGGCCCTGTCTACAACAACCGAGTGGAGATGGCCATCTTCTTCATTATCTACATCATC
  CTCATTGCCTTCTTCATGATGAACATCTTTGTGGGCTTTGTCATCGTTACCTTCCAGGAACAGGGAGAGA
  CTGAGTATAAGAACTGTGAGCTCGACAAGAACCAGCGCCAATGCGTACAGTATGCCCTGAAGGCCCGCCC
  ACTGAGGTGTTACATCCCCAAAAACCCATACCAGTATCAGGTGTGGTATGTCGTCACTTCCTCCTACTTT
  GAATACCTGATGTTTGCTCTCATCATGCTCAATACTATCTGCCTGGGCATGCAGCATTACAACCAGTCGG
  AACAGATGAACCACATCTCAGACATTCTCAACGTGGCTTTCACCATCATCTTCACCCTGGAGATGGTCCT
  CAAGCTCATAGCTTTCAAGCCCAGGGGCTATTTTGGAGATCCTTGGAATGTGTTTGACTTCCTAATCGTC
  ATCGGCAGCATCATTGACGTCATCCTGAGCGAGATCGATGACCCAGATGAGAGCGCCCGTATCTCCAGTG
  CCTTCTTCCGCCTGTTCCGGGTCATGAGACTGGTCAAGCTGCTGAACCGGGCGGAGGGTGTGCGCACCCT
  GCTGTGGACATTCATCAAGTCCTTCCAGGCCCTGCCGTACGTGGCTTTGCTGATCGTGATGCTCTTCTTC
  ATCTACGCGGTCATCGGCATGCAGATGTTCGGGAAGATCGCCATGGTGGACGGGACGCAGATAAACCGGA
  ACAACAACTTCCAGACTTTCCCACAGGCCGTGCTGCTGCTCTTCAGGTGTGCCACAGGGGAAGCCTGGCA
  AGAGATCCTGCTGGCCTGCAGCTATGGGAAGCTGTGTGACCCGGAGTCTGACTACGCACCCGGGGAGGAG
  CATACATGCGGCACCAACTTCGCCTACTACTACTTCATCAGTTTCTACATGCTCTGCGCCTTCCTGATCA
  TCAACCTCTTTGTGGCTGTCATCATGGACAACTTTGATTACCTCACAAGGGACTGGTCCATTCTGGGCCC
  TCACCACCTGGACGAGTTCAAGGCCATTTGGGCTGAGTACGACCCGGAGGCTAAGGGCAGGATCAAGCAC
  CTGGATGTGGTGACCCTGCTGAGGAGGATTCAGCCCCCTCTGGGCTTTGGGAAGTTCTGCCCACACAGGG
  TAGCATGTAAGAGGCTGGTGGGCATGAACATGCCCCTGAACAGCGATGGCACAGTCACCTTTAATGCCAC
  ACTATTTGCCCTGGTGCGCACAGCCCTCAAGATCAAGACAGAAGGCAACTTTGAACAAGCTAACGAGGAG
  CTGAGAGCCATCATCAAGAAGATCTGGAAGAGAACCAGCATGAAGCTCTTGGACCAAGTCATCCCTCCAA
  TAGGAGATGATGAGGTGACAGTAGGGAAATTCTATGCCACATTCCTCATCCAGGAGCACTTCCGGAAGTT
  CATGAAGCGCCAGGAGGAATATTATGGGTATCGACCCAAAAAGGACACAGTTCAGATACAGGCTGGATTA
  CGGACCATAGAGGAAGAGGCAGCTCCTGAGATCCACCGTGCCATCTCTGGAGACCTGACAGCAGAGGAGG
  AACTGGAGAGGGCCATGGTAGAGGCTGCCATGGAGGAGGGGATATTCCGGAGGACGGGAGGCCTGTTTGG
  TCAAGTGGACAACTTCCTGGAAAGGACCAACTCTCTGCCCCCTGTCATGGCCAACCAAAGACCCCTCCAA
  TTTGCTGAAATAGAGATGGAAGAGCTGGAGTCACCAGTCTTCTTGGAGGACTTCCCTCAGAACCCAGGCA
  CCCACCCTCTTGCTCGTGCCAATACCAACAACGCCAATGCCAATGTTGCCTATGGCAACAGCAGCCATAG
  AAACAACCCAGTGTTTTCCAGCATCTGCTATGAACGGGAGTTCTTGGGAGAGGCGGACATGCCTGTTACC
  AGAGAAGGACCCCTCAGTCAGCCCTGCAGGGCCTCAGGACCTCACAGCAGATCTCATGTGGACAAACTGA
  AAAGACCGATGACCCAGAGAGGAATGCCTGAAGGCCAGGTGCCTCCTTCCCCGTGCCAGCTCTCCCAGGC
  AGAGCATCCTGTGCAGAAAGAGGGAAAGGGCCCCACGTCACGGTTTCTGGAAACACCCAACAGCAGGAAC
  TTTGAGGAGCACGTTCCCAGGAATTCGGCTCACAGGTGCACGGCTCCAGCTACTGCCATGCTGATCCAGG
  AGGCTCTGGTTCGAGGAGGACTAGACTCCTTAGCAGCAGATGCTAACTTCGTCATGGCAACAGGCCAGGC
  CCTGGCAGATGCCTGCCAGATGGAACCAGAGGAAGTCGAGGTGGCAGCAACAGAGCTACTGAAGCAAGAA
  TCCCCAGAGGGCGGGGCCGTGCCCTGGGAGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223026 representing NM_014193
  Red=Cloning site Green=Tags(s)
  
  MEPPSPQDEGLRKKQPKKPVPEILPRPPRALFCLTLQNPLRKACISIVEWKPFETIILLTIFANCVALAV
  YLPMPEDDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTVILE
  QVNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFMVIIY
  AIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHGITHFDNFGF
  SMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGEFTKEREKAKSRGTF
  QKLREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGGSDTESLYEIEGLNKIIQFIRHWRQWNRV
  FRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHNQPLWLTHLQDVANRVLLTLFTIEMLMKMYGLGLRQ
  YFMSIFNRFDCFVVCSGILEILLVESGAMSPLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIA
  SLLLLLFLFIIIFALLGMQLFGGRYDFEDTEVRRSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGP
  TYPGVLVCIYFIILFVCGNYILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSKGLPDKSEEERAT
  VTKKLEQKSKGEGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPVSPRPRPLAELQLKEKA
  VPIPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFT
  AVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLRAINR
  AKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFACIGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDP
  TQIELRPRQWIHNDFHFDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYII
  LIAFFMMNIFVGFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYVVTSSYF
  EYLMFALIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMVLKLIAFKPRGYFGDPWNVFDFLIV
  IGSIIDVILSEIDDPDESARISSAFFRLFRVMRLVKLLNRAEGVRTLLWTFIKSFQALPYVALLIVMLFF
  IYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEILLACSYGKLCDPESDYAPGEE
  HTCGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKH
  LDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEE
  LRAIIKKIWKRTSMKLLDQVIPPIGDDEVTVGKFYATFLIQEHFRKFMKRQEEYYGYRPKKDTVQIQAGL
  RTIEEEAAPEIHRAISGDLTAEEELERAMVEAAMEEGIFRRTGGLFGQVDNFLERTNSLPPVMANQRPLQ
  FAEIEMEELESPVFLEDFPQNPGTHPLARANTNNANANVAYGNSSHRNNPVFSSICYEREFLGEADMPVT
  REGPLSQPCRASGPHSRSHVDKLKRPMTQRGMPEGQVPPSPCQLSQAEHPVQKEGKGPTSRFLETPNSRN
  FEEHVPRNSAHRCTAPATAMLIQEALVRGGLDSLAADANFVMATGQALADACQMEPEEVEVAATELLKQE
  SPEGGAVPWEP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014193
• ORF Size: 5493 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_014193.2, NP_055008.2
• RefSeq Size: 6188
• RefSeq ORF: 5496
• Locus ID: 12292
• MW: 208.9 kDa