Sphk2 (NM_001172561) Mouse Tagged ORF Clone

CAT#: MR223097

Sphk2 (Myc-DDK-tagged) - Mouse sphingosine kinase 2 (Sphk2), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sphk2
• Synonyms: C76851
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223097 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCACCACCACTACTGCCAGTGGCTGCCAGCACTCCAATCCTGCACGGCGAGTTTGGTTCCTACC
  CGGCCAACGGCCCACGGTTTGCCCTCACCCTCACAACACAAGCCCTACACATACAGCGACTACGCCCAAA
  GCCAGAAGCCCGGCCCCGAGATGGTCTAGTCTCTCTGGATGAGGTCTCGGGCTGTGGCACCCTGCAGAGC
  CGTAGCCCCGAGGACACTGCAGCCTACTTCTGCATCTACACCTACCCACGTGGCCGTCGAGGGGGCCGGC
  GCAGAGCTACGCGGACCTTCCGGGCGGATGGGGCCACCACTTATGAGGAGAATCGTGCAGAGGCCCAGCG
  CTGGGCCACTGCCCTCACGTGTCTCCTCCGAGGAGTGCCTCTGTCAGGGGACCAGGAAATCACCCCTGAA
  TTGCTGCCCCGGAAGCCCAGGCTGCTCATATTGGTCAATCCCTTTGGGGGGCGGGGCCTGGCCTGGCAGC
  GCTGTATGGACCACGTGGTGCCAATGATCTCTGAAGCTGGGCTGTCCTTCAACCTCATACAGACAGAACG
  ACAGAACCATGCCCGTGAGCTGGTGCAGGGGTTAAGCCTGAGTGAGTGGGAAGGCATTGTCACTGTGTCT
  GGAGACGGGCTGCTTTACGAGGTGCTGAATGGGCTCCTTGATCGGCCAGACTGGGAGGATGCCGTGCGGA
  TGCCCATTGGTGTCCTCCCCTGTGGATCGGGCAATGCGCTAGCTGGGGCGGTGAACCATCATGGCGGGTT
  TGAGCAGGTTGTCGGTGTTGACCTGTTGCTCAACTGCTCGCTTCTTCTCTGCCGTGGTGGCAGCCATCCT
  CTGGACCTGCTCTCTGTGACGCTAGCCTCGGGATCCCGCTGTTTTTCCTTCCTGTCAGTGGCCTGGGGAT
  TCTTGTCAGATGTGGACATTCACAGTGAGCGCTTCAGGGCCCTGGGCAGCGCTCGATTCACACTGGGTGC
  AGTGCTAGGCCTGGCCTCGTTGCATACCTACCGTGGACGCCTCTCCTACCTCCCCGCTACCACAGAACCA
  GCCTTGCCCATCCCAGGCCACAGTCTGCCTCGAGCCAAGTCAGAACTAGTCTTGGCTCCAGCCCCAGCCC
  CCGCCGCCACCCACTCGCCTCTACATCGATCTGTGTCTGACCTGCCCCTGCCCCTTCCCCAGCCTGCCTT
  GGTCTCCCCTGGCTCCCCTGAGCCCCTGCCTGACCTGTCCCTCAATGGTGGTGGTCCAGAGCTGACTGGA
  GACTGGGGAGGAGCTGGGGATGCACCTCTGTCCCCAGACCCACTGCTGCCTTCATCCCCCAACGCTCTCA
  AAACAGCTCAGCTTTCACCCATCGCTGAAGGGCCCCCAGAAATGCCAGCATCTTCGGGGTTCCTGCCTCC
  CACCCACAGTGCCCCAGAAGCCTCTACCTGGGGCCCAGTGGACCACCTCCTCCCTCCCCTGGGCTCTCCA
  CTGCCCCAAGACTGGGTGACAATAGAGGGGGAGTTTGTACTCATGTTGGGCATCTTGCCGAGCCACCTCT
  GCGCAGACCTGATGGCAGCCCCACATGCACGCTTTGATGATGGCGTTGTGCACCTGTGTTGGGTGCGGAG
  CGGCATCTCACGGGCTGCACTTCTACGCATTTTGCTGGCCATGGAGCATGGAAACCACTTCAGCCTGGGC
  TGCCCCCATCTGGGCTATGCTGCAGCACGTGCCTTCCGCCTTGAACCACTCACGCCTCGTGGCCTGCTCA
  CTGTAGATGGGGAGTTAGTGGAGTATGGGCCAATACAGGCGCAGGTGCACCCAGGTCTCGCCACGCTGCT
  CACTGGGCCTGCAGGTCAAAAGCCACAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223097 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPPPLLPVAASTPILHGEFGSYPANGPRFALTLTTQALHIQRLRPKPEARPRDGLVSLDEVSGCGTLQS
  RSPEDTAAYFCIYTYPRGRRGGRRRATRTFRADGATTYEENRAEAQRWATALTCLLRGVPLSGDQEITPE
  LLPRKPRLLILVNPFGGRGLAWQRCMDHVVPMISEAGLSFNLIQTERQNHARELVQGLSLSEWEGIVTVS
  GDGLLYEVLNGLLDRPDWEDAVRMPIGVLPCGSGNALAGAVNHHGGFEQVVGVDLLLNCSLLLCRGGSHP
  LDLLSVTLASGSRCFSFLSVAWGFLSDVDIHSERFRALGSARFTLGAVLGLASLHTYRGRLSYLPATTEP
  ALPIPGHSLPRAKSELVLAPAPAPAATHSPLHRSVSDLPLPLPQPALVSPGSPEPLPDLSLNGGGPELTG
  DWGGAGDAPLSPDPLLPSSPNALKTAQLSPIAEGPPEMPASSGFLPPTHSAPEASTWGPVDHLLPPLGSP
  LPQDWVTIEGEFVLMLGILPSHLCADLMAAPHARFDDGVVHLCWVRSGISRAALLRILLAMEHGNHFSLG
  CPHLGYAAARAFRLEPLTPRGLLTVDGELVEYGPIQAQVHPGLATLLTGPAGQKPQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172561
• ORF Size: 1851 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001172561.1, NP_001166032.1
• RefSeq Size: 3481
• RefSeq ORF: 1854
• Locus ID: 56632
• MW: 65.6 kDa
• Gene Summary: This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. The gene product also enhances apoptosis in different cell types and suppresses cellular proliferation. In mast cells, the encoded protein is necessary for influx of calcium, protein kinase C activation, and cytokine production and degranulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]