Tnnt2 (NM_001130176) Mouse Tagged ORF Clone

CAT#: MR223154

Tnnt2 (Myc-DDK-tagged) - Mouse troponin T2, cardiac (Tnnt2), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tnnt2
• Synonyms: cTnT; Tnt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223154 representing NM_001130176
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACGCCGAGGAGGTGGTGGAGGAGTACGAGGAGGAACAGGAAGAGGAAGACTGGAGTGAAGAAG
  AGGAGGACGAGCAAGAGGAGGCAGTGGAGGAGGAGGAGGCTGGTGGGGCTGAACCTGAGCCTGAGGGTGA
  GGCTGAGACAGAGGAGGCCAACGTAGAAGAGGTTGGTCCTGATGAAGAAGCCAAAGATGCTGAAGAAGGT
  CCAGTAGAGGACACCAAACCCAAGCCCAGCAGGCTCTTCATGCCCAACTTGGTGCCACCCAAGATCCCCG
  ATGGAGAGAGAGTGGACTTTGATGACATCCACAGGAAGCGCGTGGAGAAGGACCTGAATGAGCTACAGAC
  TCTGATCGAGGCTCACTTCGAGAACAGGAAGAAGGAGGAAGAGGAGCTGATTTCCCTCAAAGACAGGATC
  GAAAAGCGTCGGGCAGAGCGGGCCGAGCAGCAGCGTATTCGCAATGAGCGGGAGAAGGAAAGGCAGAACC
  GCCTGGCTGAAGAGAGGGCCCGGCGTGAGGAGGAGGAGAACAGGAGGAAGGCTGAGGATGAGGCCCGGAA
  GAAGAAGGCTCTGTCCAACATGATGCACTTTGGAGGGTACATCCAGAAGCAAGCTCAGACAGAGCGGAAG
  AGTGGGAAGAGACAGACAGAGAGAGAGAAGAAGAAGAAGATCCTGGCAGAGAGGAGGAAGGCGCTGGCCA
  TCGACCACCTGAATGAAGACCAACTGAGAGAGAAGGCCAAGGAGCTGTGGCAGAGTATTCACAACCTGGA
  GGCTGAGAAGTTCGACCTGCAGGAAAAGTTCAAGCAGCAGAAATACGAAATCAACGTTCTGCGAAACCGG
  ATCAATGACAACCAGAAAGTCTCCAAAACTCGTGGGAAGGCCAAAGTCACCGGGCGTTGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223154 representing NM_001130176
  Red=Cloning site Green=Tags(s)
  
  MSDAEEVVEEYEEEQEEEDWSEEEEDEQEEAVEEEEAGGAEPEPEGEAETEEANVEEVGPDEEAKDAEEG
  PVEDTKPKPSRLFMPNLVPPKIPDGERVDFDDIHRKRVEKDLNELQTLIEAHFENRKKEEEELISLKDRI
  EKRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKQAQTERK
  SGKRQTEREKKKKILAERRKALAIDHLNEDQLREKAKELWQSIHNLEAEKFDLQEKFKQQKYEINVLRNR
  INDNQKVSKTRGKAKVTGRWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130176
• ORF Size: 903 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001130176.1, NP_001123648.1
• RefSeq Size: 1150
• RefSeq ORF: 906
• Locus ID: 21956
• MW: 36.3 kDa
• Gene Summary: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProtKB/Swiss-Prot Function]