Trim25 (NM_009546) Mouse Tagged ORF Clone

CAT#: MR223266

Trim25 (Myc-DDK-tagged) - Mouse tripartite motif-containing 25 (Trim25)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trim25
• Synonyms: AA960166; AL022677; EFP; Zfp147
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223266 representing NM_009546
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGAATCCTCTGGCCGAGGAGCTGTCCTGCTCCGTCTGCCTGGAGCTCTTCAAGGAACCTG
  TCACCACTCCGTGCGGCCACAATTTCTGCATGTCATGCCTGGATGAGACGTGGGTCGTCCAGGGCCCGCC
  GTACCGATGCCCGCAATGTCGCAAAGTGTACCAGGTGCGCCCGCAGCTGCAAAAGAACACGGTAATGTGC
  GCGGTGGTGGAGCAGTTCCTGCAGGCCGAGCAGGCGCGGACCCCGGTGGACGACTGGACGCCTCCTGCCC
  GCTTCTCCGCCTCCAGCGCAGCCACCCAGGTGGCCTGCGACCACTGCCTGACGGAGATCGCAGTGAAGAC
  GTGCCTCGTGTGCATGGCCTCTTTCTGCCAGGAGCACTTGAGGCCTCACTTCGACAGTCCGGCCTTCCAG
  GATCACCCCCTACAGTCGCCCATTCGTGACCTTCTGCGCCGCAAATGTACCCAGCACAACCGCCTTCGGG
  AATTGTTTTGTCCCGAGCATGGCGAGTGCATCTGCCATATCTGCTTGGTAGAGCACAAGACCTGCTCCCC
  TACGACCCTAAGTCAAGCCAGCGCCGACCTGGAGTACAAACTGAGGAATAAACTCACTATCATGCACAGC
  CACATCAATGGGGCAACCAAAGCATTGGAGGATGTGAGATCCAAGCAACAGTGTGTGCAGGATTCTATGA
  AGAGGAAGATGGAACAGCTGAGACAGGAGTATATGGAAATGAAGGCTGTCATCGACGCAGCAGAGACCAG
  CTCCTTGCGCAAGTTAAAGGAGGAGGAGAAGAGAGTCTACGGAAAATTCGACACCATCTACCAGGTTCTC
  GTCAAGAAGAAGAGTGAGATGCAGAAACTGAAGGCAGAGGTTGAGCTCATTATGGACAAAGGGGATGAGT
  TTGAGTTTCTGGAGAAAGCTGCAAAATTGCAAGGAGAGTCAACAAAACCAGTCTACATCCCTAAGATAGA
  TCTGGATCATGATTTGATAATGGGAATCTACCAAGGGGCGGCGGACCTCAAGAGTGAACTGAAGCACTCC
  ATTAAGAAGCTACAGAAGAAGTCTGAGGAGCACAATGGCTCAGGTAACAAGGGAGACCAGACACAGTCCA
  CTTTTAAACCCGTACAACCTTCGAAGAAGACCATTCAAGAAAAGAAGACCAAGAAAACCCCTGTTGCTCC
  CGGCCCTCCCTCCCACTTCTCACCTAACAAGTTGCCCACCTTTGGAGCTCCTGGCCAATCACTGGATTCA
  AAAGCAACTTCCCCTGATGCCGCACCCAAAGCCAGTGCTGCACAGCCAGACTCTGTAGGAGTCAAGGCTA
  AGGTGCTAGAAAACTTCTTAACCAAGTCCAGAACGGAGCTTCTGGAGTATTTCGTGAAAGTCATCTTCGA
  CTACAATACCGCCCACAACAAAGTGTCTCTGTCAAACAAGTACACCACCGCCTCTGTGTCCGATGGGCTC
  CAGCACTACCGGTCCCATCCCCAGAGGTTCACCTACTGCTCTCAGGTCCTGGGGCTGCACTGCTACAAGA
  ACGGCATTCACTACTGGGAGGTGGAACTACAGAAGAACAACTTCTGCGGCGTAGGCATCTGCTACGGCAG
  CATGGAACGGCAGGGCCCCGAGAGCCGGCTGGGCCGCAACCCCAACTCATGGTGTGTGGAGTGGTTCAAT
  AACAAGATCTCTGCCTGGCACAACAACGTGGAGAAGACTCTGCCCTCCACCAAGGCCACTCGAGTCGGCG
  TGCTGCTCAACTGTGACCACGGCTTCGTCATTTTCTTTGCGGTCACCGAAAAGGTGCACCTGATGTACAA
  GTTCAAGGTGGACTTCACCGAAGCTCTGTACCCAGCCTTCTGGGTGTTCTCGGCCGGTACCACCCTCTCT
  ATCTGCTCCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223266 representing NM_009546
  Red=Cloning site Green=Tags(s)
  
  MAELNPLAEELSCSVCLELFKEPVTTPCGHNFCMSCLDETWVVQGPPYRCPQCRKVYQVRPQLQKNTVMC
  AVVEQFLQAEQARTPVDDWTPPARFSASSAATQVACDHCLTEIAVKTCLVCMASFCQEHLRPHFDSPAFQ
  DHPLQSPIRDLLRRKCTQHNRLRELFCPEHGECICHICLVEHKTCSPTTLSQASADLEYKLRNKLTIMHS
  HINGATKALEDVRSKQQCVQDSMKRKMEQLRQEYMEMKAVIDAAETSSLRKLKEEEKRVYGKFDTIYQVL
  VKKKSEMQKLKAEVELIMDKGDEFEFLEKAAKLQGESTKPVYIPKIDLDHDLIMGIYQGAADLKSELKHS
  IKKLQKKSEEHNGSGNKGDQTQSTFKPVQPSKKTIQEKKTKKTPVAPGPPSHFSPNKLPTFGAPGQSLDS
  KATSPDAAPKASAAQPDSVGVKAKVLENFLTKSRTELLEYFVKVIFDYNTAHNKVSLSNKYTTASVSDGL
  QHYRSHPQRFTYCSQVLGLHCYKNGIHYWEVELQKNNFCGVGICYGSMERQGPESRLGRNPNSWCVEWFN
  NKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVTEKVHLMYKFKVDFTEALYPAFWVFSAGTTLS
  ICSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009546
• ORF Size: 1902 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009546.2, NP_033572.2
• RefSeq Size: 5596
• RefSeq ORF: 1905
• Locus ID: 217069
• MW: 72.2 kDa