Casr (NM_013803) Mouse Tagged ORF Clone

CAT#: MR223303

Casr (Myc-DDK-tagged) - Mouse calcium-sensing receptor (Casr)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Casr
• Synonyms: CaR; Gprc2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223303 representing NM_013803
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATGGTTTGGCTACTGTTTGGCCCTCCTGGCTCTCACCTGGCACTCCTCTGCCTATGGGCCTGACC
  AGCGAGCCCAAAAGAAGGGGGACATTATCCTAGGAGGTCTCTTTCCTATCCATTTTGGAGTAGCAGCCAA
  AGATCAAGATCTGAAGTCAAGACCAGAGTCTGTGGAGTGCATCAGGTATAACTTCCGTGGATTCCGATGG
  TTACAAGCCATGATCTTTGCCATAGAGGAGATAAACAGCAGCCCCGCCCTTCTTCCCAACATGACTCTGG
  GATATAGGATATTCGACACCTGTAACACCGTCTCCAAGGCTCTGGAGGCCACCTTGAGTTTTGTTGCCCA
  GAACAAAATCGATTCTCTGAACCTGGATGAGTTCTGCAACTGCTCCGAGCACATCCCTTCAACCATCGCA
  GTGGTGGGAGCAACCGGCTCCGGTGTCTCCACAGCGGTAGCCAACCTGCTGGGACTTTTCTATATCCCCC
  AGGTGAGCTACGCCTCCTCTAGCAGGCTTCTCAGCAATAAGAACCAGTTCAAGTCCTTCCTCCGCACCAT
  TCCCAACGACGAACACCAGGCCACCGCGATGGCTGACATTATCGAGTATTTCCGTTGGAACTGGGTGGGC
  ACAATTGCAGCCGACGACGACTATGGCAGGCCTGGCATTGAGAAGTTCCGAGAGGAAGCCGAGGAGAGGG
  ACATCTGCATTGACTTCAGCGAGCTCATCTCCCAGTACTCTGATGAGGAAGAGATCCAGCAGGTGGTGGA
  AGTGATCCAGAACTCTACAGCCAAGGTCATTGTCGTTTTCTCCAGCGGCCCAGACCTAGAACCTCTCATC
  AAGGAGATTGTGCGCCGTAACATCACAGGCAGGATCTGGCTGGCCAGCGAGGCCTGGGCCAGTTCCTCCC
  TGATTGCTATGCCTGAGTACTTCCATGTAGTCGGGGGTACCATTGGGTTCGGTCTGAAGGCTGGGCAGAT
  TCCAGGCTTCCGAGAATTCCTACAGAAAGTCCATCCCAGGAAGTCTGTCCACAATGGTTTTGCCAAAGAG
  TTTTGGGAAGAAACATTTAATTGCCACCTGCAAGACGGCGCAAAAGGACCTTTACCCGTGGACACCTTCG
  TGAGAAGTCACGAGGAAGGCGGCAACAGGTTACTCAATAGCTCCACTGCCTTCCGACCCCTCTGCACGGG
  GGATGAAAACATCAATAGTGTCGAGACCCCTTACATGGACTACGAACATTTACGGATATCCTACAACGTG
  TACTTAGCCGTCTACTCCATTGCGCACGCCCTGCAAGATATATACACCTGCTTACCCGGAAGAGGGCTTT
  TCACCAACGGGTCCTGTGCAGACATCAAGAAGGTTGAGGCCTGGCAGGTCCTGAAACACCTACGGCACCT
  GAATTTCACCAACAACATGGGGGAGCAGGTGACCTTCGATGAGTGCGGTGACCTGGTGGGGAACTACTCC
  ATCATCAACTGGCACCTCTCCCCAGAGGACGGCTCCATTGTGTTCAAGGAAGTTGGGTACTACAATGTGT
  ATGCCAAGAAGGGAGAAAGACTGTTCATCAATGAGGGGAAGATCTTGTGGAGTGGGTTCTCCAGAGAGGT
  GCCCTTCTCCAACTGCAGCCGGGACTGTCAGGCAGGGACCAGGAAGGGCATCATTGAGGGAGAGCCCACC
  TGCTGTTTTGAGTGTGTGGAGTGTCCTGACGGCGAGTACAGTGGTGAGACAGATGCGAGTGCCTGTGACA
  AGTGCCCGGATGACTTCTGGTCCAATGAGAACCACACTTCCTGCATTGCCAAGGAGATTGAGTTCCTGGC
  GTGGACTGAGCCCTTTGGAATCGCTCTCACTCTCTTTGCGGTGCTGGGCATTTTCCTGACCGCCTTTGTG
  CTGGGCGTCTTCATCAAGTTCCGAAACACGCCCATTGTCAAGGCCACCAACCGAGAGCTGTCCTACCTCC
  TGCTCTTCTCACTCCTCTGCTGCTTCTCCAGCTCCCTGTTCTTCATTGGGGAGCCCCAGGACTGGACCTG
  CCGCCTGCGACAGCCCGCCTTCGGCATCAGCTTCGTGCTTTGTATCTCGTGCATCTTGGTGAAGACCAAT
  CGAGTCCTCCTGGTATTTGAGGCCAAAATACCCACCAGCTTCCACCGGAAGTGGTGGGGACTCAACCTGC
  AGTTCCTGCTGGTTTTCCTCTGCACCTTCATGCAGATTGTCATCTGCATCATCTGGCTCTACACGGCACC
  CCCCTCCAGCTACCGCAACCACGAGCTGGAAGACGAAATCATCTTCATCACGTGCCATGAGGGCTCACTC
  ATGGCGCTCGGCTCCCTGATCGGCTACACCTGCCTCCTGGCTGCCATCTGCTTCTTCTTTGCCTTCAAGT
  CTCGGAAGCTGCCAGAGAACTTCAACGAAGCCAAGTTCATTACCTTCAGCATGCTCATCTTCTTCATCGT
  CTGGATCTCCTTCATTCCAGCCTATGCCAGCACCTACGGCAAGTTTGTCTCTGCCGTGGAGGTGATCGCC
  ATCCTGGCAGCCAGCTTTGGCTTGCTAGCCTGCATCTTCTTCAACAAGGTCTACATCATCCTCTTCAAGC
  CTTCACGGAACACCATCGAGGAGGTGCGCTCCAGCACTGCGGCTCATGCTTTCAAAGTGGCAGCCCGCGC
  CACTCTACGCCGCCCCAACATCTCCCGGAAGCGGTCCAGCAGCCTCGGAGGCTCCACTGGGTCGATTCCC
  TCCTCCTCCATCAGCAGCAAAAGCAACAGCGAAGACCGGTTCCCACAGCCGGAGAGGCAGAAGCAACAGC
  AACCACTGGCCCTGACCCAGCAAGAACAGCAGCAGCAGCCCCTGACTCTCCAGCCGCAGCAACAGCAACA
  GCCACAGCAGCCGAGATGCAAACAGAAGGTCATCTTCGGCAGTGGCACAGTCACCTTCTCTCTGAGCTTT
  GATGAGCCTCAGAAGAATGCTATGGCCCACAGGAACTCCATGCGCCAGAACTCCCTGGAGGCCCAGAAGA
  GCAATGACACTTTGAACAGACACCAGGCCCTGCTTCCCCTACAGTGTGCAGAGGCGGACTCAGAAATGAC
  CATTCAGGAGACGGGCTTGCAAGGGCCAATGGTGGGGGACCACCAGCCAGAAATAGAAAGCCCAGATGAA
  ATGTCCCCAGCTCTGGTCATGTCCACCTCTCGGAGCTTCGTCATCAGCGGTGGAGGTAGCTCTGTGACGG
  AAAACATACTGCACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223303 representing NM_013803
  Red=Cloning site Green=Tags(s)
  
  MAWFGYCLALLALTWHSSAYGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRW
  LQAMIFAIEEINSSPALLPNMTLGYRIFDTCNTVSKALEATLSFVAQNKIDSLNLDEFCNCSEHIPSTIA
  VVGATGSGVSTAVANLLGLFYIPQVSYASSSRLLSNKNQFKSFLRTIPNDEHQATAMADIIEYFRWNWVG
  TIAADDDYGRPGIEKFREEAEERDICIDFSELISQYSDEEEIQQVVEVIQNSTAKVIVVFSSGPDLEPLI
  KEIVRRNITGRIWLASEAWASSSLIAMPEYFHVVGGTIGFGLKAGQIPGFREFLQKVHPRKSVHNGFAKE
  FWEETFNCHLQDGAKGPLPVDTFVRSHEEGGNRLLNSSTAFRPLCTGDENINSVETPYMDYEHLRISYNV
  YLAVYSIAHALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRHLNFTNNMGEQVTFDECGDLVGNYS
  IINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEGKILWSGFSREVPFSNCSRDCQAGTRKGIIEGEPT
  CCFECVECPDGEYSGETDASACDKCPDDFWSNENHTSCIAKEIEFLAWTEPFGIALTLFAVLGIFLTAFV
  LGVFIKFRNTPIVKATNRELSYLLLFSLLCCFSSSLFFIGEPQDWTCRLRQPAFGISFVLCISCILVKTN
  RVLLVFEAKIPTSFHRKWWGLNLQFLLVFLCTFMQIVICIIWLYTAPPSSYRNHELEDEIIFITCHEGSL
  MALGSLIGYTCLLAAICFFFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYASTYGKFVSAVEVIA
  ILAASFGLLACIFFNKVYIILFKPSRNTIEEVRSSTAAHAFKVAARATLRRPNISRKRSSSLGGSTGSIP
  SSSISSKSNSEDRFPQPERQKQQQPLALTQQEQQQQPLTLQPQQQQQPQQPRCKQKVIFGSGTVTFSLSF
  DEPQKNAMAHRNSMRQNSLEAQKSNDTLNRHQALLPLQCAEADSEMTIQETGLQGPMVGDHQPEIESPDE
  MSPALVMSTSRSFVISGGGSSVTENILHS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013803
• ORF Size: 3237 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013803.1, NM_013803.2, NM_013803.3, NP_038831.2
• RefSeq Size: 4549
• RefSeq ORF: 3240
• Locus ID: 12374
• MW: 121.4 kDa
• Gene Summary: Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. [UniProtKB/Swiss-Prot Function]