Flt3 (NM_010229) Mouse Tagged ORF Clone

CAT#: MR223373

Flt3 (Myc-DDK-tagged) - Mouse FMS-like tyrosine kinase 3 (Flt3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Flt3
• Synonyms: B230315G04; CD135; Flk-2; Flk2; Flt-3; Ly72; wmfl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223373 representing NM_010229
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGCGTTGGCGCAGCGCAGCGACCGGCGGCTGCTGCTGCTTGTTGTTTTGTCAGTAATGATTCTTG
  AGACCGTTACAAACCAAGACCTGCCTGTGATCAAGTGTGTTTTAATCAGTCATGAGAACAATGGCTCATC
  AGCGGGAAAGCCATCATCGTACCGAATGGTGCGAGGATCCCCAGAAGACCTCCAGTGTGCCCCGAGGCGC
  CAGAGTGAAGGGACGGTATATGAAGCGGCCACCGTGGAGGTGGCCGAGTCTGGGTCCATCACCCTGCAAG
  TGCAGCTCGCCACCCCAGGGGACCTTTCCTGCCTCTGGGTCTTTAAGCACAGCTCCCTGGGCTGCCAGCC
  GCACTTTGATTTACAAAACAGAGGAATCGTTTCCATGGCCATCTTGAACGTGACAGAGACCCAGGCAGGA
  GAATACCTACTCCATATTCAGAGCGAAGCCGCCAACTACACAGTACTGTTCACAGTGAATGTAAGAGATA
  CACAGCTGTACGTGCTAAGAAGACCTTACTTTAGGAAGATGGAAAACCAGGACGCACTGCTCTGCATCTC
  CGAGGGTGTTCCAGAGCCCACTGTGGAGTGGGTGCTCTGCAGCTCCCACAGGGAAAGCTGTAAAGAAGAA
  GGCCCTGCTGTTGTCAGAAAGGAGGAAAAGGTACTTCATGAGTTGTTCGGAACAGACATCAGATGCTGTG
  CTAGAAATGCACTGGGCCGCGAATGCACCAAGCTGTTCACCATAGATCTAAACCAGGCTCCTCAGAGCAC
  ACTGCCCCAGTTATTCCTGAAAGTGGGGGAACCCTTGTGGATCAGGTGTAAGGCCATCCATGTGAACCAT
  GGATTCGGGCTCACCTGGGAGCTGGAAGACAAAGCCCTGGAGGAGGGCAGCTACTTTGAGATGAGTACCT
  ACTCCACAAACAGGACCATGATTCGGATTCTCTTGGCCTTTGTGTCTTCCGTGGGAAGGAACGACACCGG
  ATATTACACCTGCTCTTCCTCAAAGCACCCCAGCCAGTCAGCGTTGGTGACCATCCTAGAAAAAGGGTTT
  ATAAACGCTACCAGCTCGCAAGAAGAGTATGAAATTGACCCGTACGAAAAGTTCTGCTTCTCAGTCAGGT
  TTAAAGCGTACCCACGAATCCGATGCACGTGGATCTTCTCTCAAGCCTCATTTCCTTGTGAACAGAGAGG
  CCTGGAGGATGGGTACAGCATATCTAAATTTTGCGATCATAAGAACAAGCCAGGAGAGTACATATTCTAT
  GCAGAAAATGATGACGCCCAGTTCACCAAAATGTTCACGCTGAATATAAGAAAGAAACCTCAAGTGCTAG
  CAAATGCCTCAGCCAGCCAGGCGTCCTGTTCCTCTGATGGCTACCCGCTACCCTCTTGGACCTGGAAGAA
  GTGTTCGGACAAATCTCCCAATTGCACGGAGGAAATCCCAGAAGGAGTTTGGAATAAAAAGGCTAACAGA
  AAAGTGTTTGGCCAGTGGGTGTCGAGCAGTACTCTAAATATGAGTGAGGCCGGGAAAGGGCTTCTGGTCA
  AATGCTGTGCGTACAATTCTATGGGCACGTCTTGCGAAACCATCTTTTTAAACTCACCAGGCCCCTTCCC
  TTTCATCCAAGACAACATCTCCTTCTATGCGACCATTGGGCTCTGTCTCCCCTTCATTGTTGTTCTCATT
  GTGTTGATCTGCCACAAATACAAAAAGCAATTTAGGTACGAGAGTCAGCTGCAGATGATCCAGGTGACTG
  GCCCCCTGGATAACGAGTACTTCTACGTTGACTTCAGGGACTATGAATATGACCTTAAGTGGGAGTTCCC
  GAGAGAGAACTTAGAGTTTGGGAAGGTCCTGGGGTCTGGCGCTTTCGGGAGGGTGATGAACGCCACGGCC
  TATGGCATTAGTAAAACGGGAGTCTCAATTCAGGTGGCGGTGAAGATGCTAAAAGAGAAAGCTGACAGCT
  GTGAAAAAGAAGCTCTCATGTCGGAGCTCAAAATGATGACCCACCTGGGACACCATGACAACATCGTGAA
  TCTGCTGGGGGCATGCACACTGTCAGGGCCAGTGTACTTGATTTTTGAATATTGTTGCTATGGTGACCTC
  CTCAACTACCTAAGAAGTAAAAGAGAGAAGTTTCACAGGACATGGACAGAGATTTTTAAGGAACATAATT
  TCAGTTTTTACCCTACTTTCCAGGCACATTCAAATTCCAGCATGCCTGGTTCACGAGAAGTTCAGTTACA
  CCCGCCCTTGGATCAGCTCTCAGGGTTCAATGGGAATTTAATTCATTCTGAAGATGAGATTGAATATGAA
  AACCAGAAGAGGCTGGCAGAAGAAGAGGAGGAAGATTTGAACGTGCTGACGTTTGAAGACCTCCTTTGCT
  TTGCGTACCAAGTGGCCAAAGGCATGGAATTCCTGGAGTTCAAGTCGTGTGTCCACAGAGACCTGGCAGC
  CAGGAATGTGTTGGTCACCCACGGGAAGGTGGTGAAGATCTGTGACTTTGGACTGGCCCGAGACATCCTG
  AGCGACTCCAGCTACGTCGTCAGGGGCAACGCACGGCTGCCGGTGAAGTGGATGGCACCTGAGAGCTTAT
  TTGAAGGGATCTACACAATCAAGAGTGACGTCTGGTCCTACGGCATCCTTCTCTGGGAGATATTTTCACT
  GGGTGTGAACCCTTACCCTGGCATTCCTGTCGACGCTAACTTCTATAAACTGATTCAGAGTGGATTTAAA
  ATGGAGCAGCCATTCTATGCCACAGAAGGGATATACTTTGTAATGCAATCCTGCTGGGCTTTTGACTCAA
  GGAAGCGGCCATCCTTCCCCAACCTGACTTCATTTTTAGGATGTCAGCTGGCAGAGGCAGAAGAAGCGAT
  GTATCAGAACATGGGTGGCAACGTCCCAGAACATCCATCCATCTACCAAAACAGGCGGCCCCTCAGCAGA
  GAGGCAGGCTCAGAGCCGCCATCGCCACAGGCCCAGGTGAAGATTCACGGAGAAAGAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223373 representing NM_010229
  Red=Cloning site Green=Tags(s)
  
  MRALAQRSDRRLLLLVVLSVMILETVTNQDLPVIKCVLISHENNGSSAGKPSSYRMVRGSPEDLQCAPRR
  QSEGTVYEAATVEVAESGSITLQVQLATPGDLSCLWVFKHSSLGCQPHFDLQNRGIVSMAILNVTETQAG
  EYLLHIQSEAANYTVLFTVNVRDTQLYVLRRPYFRKMENQDALLCISEGVPEPTVEWVLCSSHRESCKEE
  GPAVVRKEEKVLHELFGTDIRCCARNALGRECTKLFTIDLNQAPQSTLPQLFLKVGEPLWIRCKAIHVNH
  GFGLTWELEDKALEEGSYFEMSTYSTNRTMIRILLAFVSSVGRNDTGYYTCSSSKHPSQSALVTILEKGF
  INATSSQEEYEIDPYEKFCFSVRFKAYPRIRCTWIFSQASFPCEQRGLEDGYSISKFCDHKNKPGEYIFY
  AENDDAQFTKMFTLNIRKKPQVLANASASQASCSSDGYPLPSWTWKKCSDKSPNCTEEIPEGVWNKKANR
  KVFGQWVSSSTLNMSEAGKGLLVKCCAYNSMGTSCETIFLNSPGPFPFIQDNISFYATIGLCLPFIVVLI
  VLICHKYKKQFRYESQLQMIQVTGPLDNEYFYVDFRDYEYDLKWEFPRENLEFGKVLGSGAFGRVMNATA
  YGISKTGVSIQVAVKMLKEKADSCEKEALMSELKMMTHLGHHDNIVNLLGACTLSGPVYLIFEYCCYGDL
  LNYLRSKREKFHRTWTEIFKEHNFSFYPTFQAHSNSSMPGSREVQLHPPLDQLSGFNGNLIHSEDEIEYE
  NQKRLAEEEEEDLNVLTFEDLLCFAYQVAKGMEFLEFKSCVHRDLAARNVLVTHGKVVKICDFGLARDIL
  SDSSYVVRGNARLPVKWMAPESLFEGIYTIKSDVWSYGILLWEIFSLGVNPYPGIPVDANFYKLIQSGFK
  MEQPFYATEGIYFVMQSCWAFDSRKRPSFPNLTSFLGCQLAEAEEAMYQNMGGNVPEHPSIYQNRRPLSR
  EAGSEPPSPQAQVKIHGERS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010229
• ORF Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010229.2, NP_034359.2
• RefSeq Size: 3664
• RefSeq ORF: 3003
• Locus ID: 14255
• MW: 113.9 kDa