Rasgrf1 (NM_001039655) Mouse Tagged ORF Clone

CAT#: MR223621

Rasgrf1 (Myc-DDK-tagged) - Mouse RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rasgrf1
• Synonyms: AI844718; CDC25; CDC25Mm; Gnrp; Grf1; Grfbeta; p190; P190-A; p190RhoGEF; Ras-GRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223621 representing NM_001039655
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAAAGCCATCCGGCTTAACGATGGCCACGTCGTGACCCTGGGACTGCTGGCACAGAAGGACGGCA
  CGCGCAAAGGCTACCTGAGCAAGAGGAGTGCGGACAACCCAAAATGGCAAACCAAGTGGTTTGCCCTGCT
  CCAGAACCTGCTTTTCTACTTCGAAAGCGACTCCAGCCCTCGGCCCTCGGGGCTCTACCTGCTCGAGGGA
  AGTATCTGTAAACGCGCGCCCTCCCCCAAGCGAGGGACCTCCTCCAAGGAGTCCGGCGAGAAACAGCGTC
  ATAGCAGGAGTCGACGTCGACGTAATGTTCTGAGAAGACAACCAACCAGGATCTCAAAAACATTCAGGCT
  GGTGGCAATTGAATTGACTGGGATGACAGCCACCCTTGAAGTCTCCTTCTCCATAATTAGGACAGAGATC
  GGTACCGTTGCTCCAGCTCCACAGACATGGATGGGAAAGCCAGAAGACTTGACAACGCTGCGTGACAGAG
  TGGAGTCGAAGCGTCTCTTCCACCATCCCTGTAGATTGATCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223621 representing NM_001039655
  Red=Cloning site Green=Tags(s)
  
  MQKAIRLNDGHVVTLGLLAQKDGTRKGYLSKRSADNPKWQTKWFALLQNLLFYFESDSSPRPSGLYLLEG
  SICKRAPSPKRGTSSKESGEKQRHSRSRRRRNVLRRQPTRISKTFRLVAIELTGMTATLEVSFSIIRTEI
  GTVAPAPQTWMGKPEDLTTLRDRVESKRLFHHPCRLII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039655
• ORF Size: 534 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001039655.1, NP_001034744.1
• RefSeq Size: 1000
• RefSeq ORF: 537
• Locus ID: 19417
• MW: 20.8 kDa