Brd8 (NM_030147) Mouse Tagged ORF Clone
CAT#: MR223690
- TrueORF®
Brd8 (Myc-DDK-tagged) - Mouse bromodomain containing 8 (Brd8)
"NM_030147" in other vectors (4)
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Product Images
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Brd8 |
Synonyms | 2610007E11Rik; 4432404P07Rik; SMAP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR223690 representing NM_030147
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCGGGACAGGCAAGCACAAGCTGCTGAGTACTGGCCCAACAGAGCCATGGTCCATCCGAGAGA AGTTGTGTTTAGCATCTTCTGTCATGAGGAGTGGGGATCAGAACTGGGTATCAGTTAGCAGAGCAATCAA GCCCTTTGCAGAACCTGGCCGGCCTCCAGACTGGTTCTCTCAAAAACATTGTGCTTCTCAGTATTCTGAG CTCTTAGAGACTACTGAGACTCCAAAACGGAAACGGGGTGAAAAAGGAGAAGTGGTAGAAACTGTTGAAG ATGTTATTGTTCGCAAACTGACTGCTGAGAGAGTTGAAGAACTGAAGAAAGTCATAAAGGAGACACAGGA GAGATACAGGCGTCTGAAAAGAGATGCAGAACTAATCCAAGCCGGGCACATGGACAGCAGACTGGATGAG CTCTGCAATGACATTGCAATGAAAAAGAAATTGGAAGAGGAAGAGGCTGAAGTAAAAAGGAAGGCCACCG ATGCTGCGTACCAGGCGCGACAAGCAGTGAAAACGCCTCCTCGAAGGTTACCGACTGTGATGGTCCGCTC TCCTGTAGACTCTGCCTCCCCAGGAGGTGATTACCCACTTGGAGACTTGACTCCAACCACTATGGAAGAG GCTACCTCTGGGGTAACCCCTGGGACTTTGCCGAGTACCCCAGTCACCTCGTTTCCTGGGATTCCTGACA CCCTTCCTCCAGGCTCTGCACCCTTAGAAGCCCCCATGACCCCAATAACAGATGATTCACCCCAGAAAAA GATGCTTGGACAGAAAGCAACTCCACCCCCCTCCCCTCTGCTGTCAGAGCTCTTGAAGAAGGGCAGCCTC CTGCCTACTAGCCCCAGACTGGTGAATGAGAGTGAAATGCCTGTACCCCCTGGCCATCTGAACAGCACAG GGGTTCTCTTGGAGGTAGGAGGGGTTCTTCCCATGATACATGGTGGGGAGATACAGCCAACAACCAGTGC TGTGGCGGCCTCCCCGGCTGCCTCAGGTGCTCCCACTCTTTCCCGGCTTTTAGAAGCTGGGCCCACACAG TTCACCACTCCTCTTCCTTCCTTCACTACTGTTGCCAGTGAGCCGCCAGTTAAGCTTGTGCCACCCCCTG TAGAGTCTGTGTCCCAGGCTACCATTGTCATGATGCCCGCGCTGCCAGCACCATCCTCTGCTGCGGCTGT CTCCACTTCTGAAAGTGGAGCTCCAGTGAGCCAGCCTGAGCCCTGTGTACCCCTGGAAGCTGTGGGGGAT CCACATACTGTGACTGTTTCCATGGATAGCAATGAAATCTCCATGATCATTAATTCCATCAAAGAAGAGT GTTTCCGCTCAGGGGTAGCAGAGGCTCCTGGTGGATCAAAGGCTCCAAGCATAGATGGGAAGGAAGACTT GGATCTAGCGGAGAAGATGGATATTGCTGTGTCTTACACAGGTGAAGAGTTGGACTTTGAAACAGTTGGA GACATCATTGCCATCATTGAGGACAAGGTGGATGATCACCCTGAAGTGCTGGATGTGGCAGCGGTAGAAG CGGCGTTATCGTTCTGTGAAGAGAATGATGACCCTCAGTCCCTGCCTGGCCCCTGGGAGCACCCTATCCA GCAGGAGCGCGACAAGCCAGTACCTCTGCCAGCACCAGAGATGACAGTCAAACAAGAGAGGCTAGACTTC GAGGAATCAGAAAACAAAGGGCTCCATGACCTGGTGGACATCAGGGATTCCGGTGTTGAGATTAAGGTGG AACCCACAGAGCCAGAGCCAGGCATGTCTGGGGCTGAGATAGTAGCTGGAGTTGGTCCAGTTCCAAGTAT GGAGCCACCAGAACTCAGGAGTCAAGACTCAGATGAAGAACCTAGAAGTTCTGCAGCTGGAGACATTGGT GAGGCAGATGGTTCCAGTGGGAAAGGCGATGAGAGGCCACTTTCAGCTGTGAAGACAGAGGCATCCCCTG AGAGCATGTTGTCTCCATCACATGGCTCAAATCTTATTGAAGATCCTTTAGAGGCAGAGACTCAACACAA GTTTGAAATGTCAGACTCATTGAAAGAAGAATCAGGGACTATTTTTGGAAGCCAGATAAAGGATGCCCCA GGTGACGATGAGGAAGAAGATGGAGTCAGTGAAGCAGCTAGCCTAGAGGAGCCTAAGGAAGAGGATCAAG GAGAAGGCTATTTGTCTGAGATGGATAATGAGCCCCCTGTGAGTGAGAGTGATGATGGCTTTAGTATACA TAACGCCACACTGCAGTCACACACTCTGGCAGACTCCATCCCAAGCAGCCCTGCCTCCTCCCAGTTTTCC GTGTGCAGTGAAGATCAAGAAGCAATTCAGGCTCAGAAAATATGGAAGAAAGCCATCATGCTTGTATGGA GGGCTGCAGCAAATCATAGGTATGCCAATGTGTTCCTGCAACCTGTTACAGATGACATAGCTCCTGGTTA CCATAGCATTGTACAGAGGCCTATGGATTTGTCAACTATAAAGAAAAACATTGAAAATGGACTGATCCGA AGCACAGCTGAGTTTCAGCGTGACATCATGCTGATGTTTCAGAATGCTGTTATGTACAATAGCTCAGACC ATGATGTCTATCACATGGCAGTAGAGATGCAGAGAGATGTCTTGGAACAGATCCAGCAATTCTTGGCCAC ACAGTTGATTATGCAAACATCTGAGTCTGGAATCAGTGCTAAAAGTCTCCGGGGGAGAGACTCTACCCGA AAACAAGATGCTTCAGAGAAGGACAGTGTCCCCATGGGCTCTCCTGCCTTCCTTCTCTCTCTCTTTGATG GGGGAACCAGGGGACGCCGCTGTGCCATTGAAGCAGATATGAAGATGAAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223690 representing NM_030147
Red=Cloning site Green=Tags(s) MATGTGKHKLLSTGPTEPWSIREKLCLASSVMRSGDQNWVSVSRAIKPFAEPGRPPDWFSQKHCASQYSE LLETTETPKRKRGEKGEVVETVEDVIVRKLTAERVEELKKVIKETQERYRRLKRDAELIQAGHMDSRLDE LCNDIAMKKKLEEEEAEVKRKATDAAYQARQAVKTPPRRLPTVMVRSPVDSASPGGDYPLGDLTPTTMEE ATSGVTPGTLPSTPVTSFPGIPDTLPPGSAPLEAPMTPITDDSPQKKMLGQKATPPPSPLLSELLKKGSL LPTSPRLVNESEMPVPPGHLNSTGVLLEVGGVLPMIHGGEIQPTTSAVAASPAASGAPTLSRLLEAGPTQ FTTPLPSFTTVASEPPVKLVPPPVESVSQATIVMMPALPAPSSAAAVSTSESGAPVSQPEPCVPLEAVGD PHTVTVSMDSNEISMIINSIKEECFRSGVAEAPGGSKAPSIDGKEDLDLAEKMDIAVSYTGEELDFETVG DIIAIIEDKVDDHPEVLDVAAVEAALSFCEENDDPQSLPGPWEHPIQQERDKPVPLPAPEMTVKQERLDF EESENKGLHDLVDIRDSGVEIKVEPTEPEPGMSGAEIVAGVGPVPSMEPPELRSQDSDEEPRSSAAGDIG EADGSSGKGDERPLSAVKTEASPESMLSPSHGSNLIEDPLEAETQHKFEMSDSLKEESGTIFGSQIKDAP GDDEEEDGVSEAASLEEPKEEDQGEGYLSEMDNEPPVSESDDGFSIHNATLQSHTLADSIPSSPASSQFS VCSEDQEAIQAQKIWKKAIMLVWRAAANHRYANVFLQPVTDDIAPGYHSIVQRPMDLSTIKKNIENGLIR STAEFQRDIMLMFQNAVMYNSSDHDVYHMAVEMQRDVLEQIQQFLATQLIMQTSESGISAKSLRGRDSTR KQDASEKDSVPMGSPAFLLSLFDGGTRGRRCAIEADMKMKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_030147 |
ORF Size | 2853 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_030147.2, NM_030147.3, NP_084423.2 |
RefSeq Size | 4677 |
RefSeq ORF | 2856 |
Locus ID | 78656 |
MW | 103.1 kDa |
Gene Summary | May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. [UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201594 | Brd8 (untagged) - Mouse bromodomain containing 8 (Brd8), (10ug) |
USD 210.00 |
|
MG223690 | Brd8 (GFP-tagged) - Mouse bromodomain containing 8 (Brd8), (10ug) |
USD 1,100.00 |
|
MR223690L3 | Lenti ORF clone of Brd8 (Myc-DDK-tagged) - Mouse bromodomain containing 8 (Brd8) |
USD 1,200.00 |
|
MR223690L4 | Lenti ORF clone of Brd8 (mGFP-tagged) - Mouse bromodomain containing 8 (Brd8) |
USD 1,200.00 |
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