Hk3 (NM_001033245) Mouse Tagged ORF Clone

CAT#: MR223735

Hk3 (Myc-DDK-tagged) - Mouse hexokinase 3 (Hk3), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hk3
• Synonyms: HK-III; HK III
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR223735 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCATTGGGCCTTCTGGCCTGCATCCTGGAGAAAGAGCCTCAGTCTGCCCACACGAGGGCGTGC
  CAAGGCCCTCAGGTAGCTTAGAACTGGAATGCTTGCAGCAATTCAAGGTGACCAGGACACAGTTGCAGCA
  GATCCAAGCCAGTCTCCTGTGCTCCATGGAGCAGGCACTGAAGGGACAGGACAGTCCCGCTCCTTCTGTC
  CGGATGCTGCCCACATACGTGAGGTCCACACCACACGGCACCGAGCAAGGGGATTTCCTGGTGCTGGAGC
  TGGGGGCCACAGGAGCATCACTACGTGTGTTGTGGGTAACACTGACAGGCACCAAAGAATGCCGGGTGGA
  GCCCAGGAGCCGGGAGTTTGTGATTCCTCAAGAGGTGATCCTAGGTGCTGGCCAGCAGCTCTTTGACTTT
  GCTGCCCGATGCCTCTCTGAATTCCTGGATGCATACCCCGTGGAGAATCAGGGTCTGAAGCTTGGGTTCA
  ATTTCTCTTTTCCTTGTCACCAGACAGGCTTGGACAGGAGCACCCTCATTTCCTGGACAAAAGGTTTTAG
  GTGCAGCGGCGTGGAAGGCCAGGATGTGGTCCAGTTGCTAAGAGATGCCATTCAGAGGCAGGGGACCTAC
  AGGATTGATGTGGTAGCCATGGTGAATGACACGGTGGGCACCATGATGGGCTGTGAGCTGGGCACCAGGC
  CGTGTGAAGTCGGGCTGATTGTAGACACCGGTACCAACGCGTGCTACATGGAGGAGGCGCGGCACGTGGC
  CGCTCTGGATGAGGACCGCGGCCGCACCTGTGTCAGCATCGAGTGGGGCTCCTTCTATGACGAAGATGCT
  CTAGGGCCAGTACTGACCACCTTCGACTCTGCCCTGGACCGCGAGTCCCTGACTCCTGGTGCTCAAAGGT
  TTGAGAAGATGATTGGAGGCCTTTACCTGGGTGAGCTGGTGAGGCTGGTTCTGGTCCACTTGACCCAGCA
  CGGGGTCCTCTTTGATGGCTGCGCCTCTCCTGCGTTGCTGAGTCAAGGCTGTATCCTCCTGGACCACGTG
  GCTGAGATGGAGGACACCGCCACTGGGACAGCCCGCGTCCACACCATCCTGCAGGACTTGGGTCTGAGCC
  CTCGGGCCTCAGATGCTGAACTGGTGCAATGTGTGTGCGTGGCTGTGTGCACACGGGCTGCCCAGCTCTG
  TGCGGCTGCCCTGGCTGCAGTCCTCTCCCGCCTCCAGCACAGCAGGGAGCAGCAGACATTGCAAGTGGCT
  GTGGCCACTGGAGGGCGAGTGTTTGAACGACACCCCAGGTTCCTCCGAATCCTAAAGGAGACAGTAATGC
  TCCTGGCCCCAAACTGTGATGTCTCCTTCATCCCCTCTGTGGATGGTGGTGGCCGGGGTGTGGCAATGGT
  GACAGCTGTGGCTGCCCGCCTGGCTGCTCACAGGCGCATCCTGGAAGAGACCCTGGCACCATTTCAGCTG
  ACCTTGGAGCAGCTGACAGTGGTGCAGGCACAAATGCGGGAAGCCATGATCAGGGGGCTTCAAGGAGAGG
  CCTCCTCCCTCCGCATGCTGCCCACTTACGTCCGAGCGACGCCCGACGGCAGTGAGCGAGGTGATTTCCT
  GGCCTTGGACCTAGGGGGCACCAACTTCCGGGTCCTGTTGGTACGAGTGGCCGAGGGCAGTGTGCAGATC
  ATCAACCAGGTCTACTCCATTCCTGAGTGTAGAGCCCAGGGCTCTGGACAGAAGCTCTTTGACCATATTG
  TGGACTGCATCGTGGACTTCCAGAAGAGGCAGGGCCTTAGTGGACAGAGCCTACCCCTGGGTTTCACCTT
  CTCTTTCCCATGCAAGCAGCTTGGCCTGGACCAGGGCATCCTCCTGAATTGGACTAAGGGGTTCAATGCA
  TCAGGCTGTGAGGGCCAGGATGTTGTGTATCTATTACGGGAAGCCATTAGGCGCAGACAGGCAGTGGAGC
  TGAATGTGGTTGCCATTGTCAATGACACGGTGGGGACCATGATGTCCTGTGGCTATGATGATCCCCGTTG
  TGAGATGGGCCTCATTGTCGGAACCGGCACCAACGCCTGTTACATGGAAGAGCTCCGCAATGTGGCAAGT
  GTGCCTGGGGACTCGGGCCTCATGTGCATCAACATGGAGTGGGGTGCCTTTGGGGATGATGGCTCACTGG
  GCACACTCAGCCCCCGCTTTGACACCAGTGTGGACCAGGCATCCATCAATCCAGGCAAACAGAGGTTTGA
  GAAAATGATCAGCGGTATGTACTTGGGGGAGATCGTCCGCCATATCCTCCTGCACTTAACCAATCTCGGA
  GTTCTCTTCCGAGGCCAGAAGACTCAATGCCTTCAGGCCAGGGACATCTTCAAGACTAAGTTCCTCTCTG
  AGATCGAAAGTGATAGCCTGGCCCTGCGTCAGGTCCGAGCCATCCTGGAAGATCTGGGGCTGACTCTGAC
  ATCTGATGACGCCTTGATGGTCCTGGAAGTGTGCCAGGCTGTGTCTCGCAGGGCTGCCCAACTCTGTGGG
  GCAGGTGTGGCTGCCGTGGTGGAGAAGATTCGGGAGAACCGGGGCCTGCAGGAGCTGACAGTGTCTGTGG
  GAGTAGATGGGACACTCTACAAGCTACATCCTCACTTCTCCAAGCTGGTGTCGGCAACAGTCCGGAAGCT
  AGCCCCTCAGTGCACCGTCACCTTTTTGCAGTCGGAGGATGGGTCTGGCAAAGGCGCAGCTTTGGTTACT
  GCTGTTGCTTGTCGCCTTACCCAGATGGCCCACGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR223735 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATIGPSGLHPGERASVCPHEGVPRPSGSLELECLQQFKVTRTQLQQIQASLLCSMEQALKGQDSPAPSV
  RMLPTYVRSTPHGTEQGDFLVLELGATGASLRVLWVTLTGTKECRVEPRSREFVIPQEVILGAGQQLFDF
  AARCLSEFLDAYPVENQGLKLGFNFSFPCHQTGLDRSTLISWTKGFRCSGVEGQDVVQLLRDAIQRQGTY
  RIDVVAMVNDTVGTMMGCELGTRPCEVGLIVDTGTNACYMEEARHVAALDEDRGRTCVSIEWGSFYDEDA
  LGPVLTTFDSALDRESLTPGAQRFEKMIGGLYLGELVRLVLVHLTQHGVLFDGCASPALLSQGCILLDHV
  AEMEDTATGTARVHTILQDLGLSPRASDAELVQCVCVAVCTRAAQLCAAALAAVLSRLQHSREQQTLQVA
  VATGGRVFERHPRFLRILKETVMLLAPNCDVSFIPSVDGGGRGVAMVTAVAARLAAHRRILEETLAPFQL
  TLEQLTVVQAQMREAMIRGLQGEASSLRMLPTYVRATPDGSERGDFLALDLGGTNFRVLLVRVAEGSVQI
  INQVYSIPECRAQGSGQKLFDHIVDCIVDFQKRQGLSGQSLPLGFTFSFPCKQLGLDQGILLNWTKGFNA
  SGCEGQDVVYLLREAIRRRQAVELNVVAIVNDTVGTMMSCGYDDPRCEMGLIVGTGTNACYMEELRNVAS
  VPGDSGLMCINMEWGAFGDDGSLGTLSPRFDTSVDQASINPGKQRFEKMISGMYLGEIVRHILLHLTNLG
  VLFRGQKTQCLQARDIFKTKFLSEIESDSLALRQVRAILEDLGLTLTSDDALMVLEVCQAVSRRAAQLCG
  AGVAAVVEKIRENRGLQELTVSVGVDGTLYKLHPHFSKLVSATVRKLAPQCTVTFLQSEDGSGKGAALVT
  AVACRLTQMAHV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033245
• ORF Size: 2769 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033245.1, NM_001033245.2, NM_001033245.3, NM_001033245.4, NP_001028417.1
• RefSeq Size: 3219 bp
• RefSeq ORF: 2769 bp
• Locus ID: 212032
• Cytogenetics: 13 B1
• MW: 100 kDa