Ghrhr (NM_001003685) Mouse Tagged ORF Clone

CAT#: MR224048

Ghrhr (Myc-DDK-tagged) - Mouse growth hormone releasing hormone receptor (Ghrhr)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ghrhr
• Synonyms: Ghrfr; lit; little
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224048 representing NM_001003685
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGCCTGATGTGGGCTACCCGTATCCTCTGCTTGCTGAGCCTGTGCGGAGTTACACTGGGTCACC
  TCCACCTAGAATGTGACTTCATCACTCAGCTGAGAGACGATGAGCTTGCATGTCTTCAGGCGGCAGAGGG
  GACCAACAACACCTCCCTGGGATGCCCTGGGACCTGGGATGGGCTGCTGTGCTGGCCCCCGACAGGCTCT
  GGCCAGTGGGTCTCTCTCCCCTGCCCTGAATTCTTCTCTCACTTCGGCTCAGACACAGGGTTTGTGAAGA
  GGGACTGCACCATCACTGGCTGGTCTAATCCCTTCCCACCATACCCTGTGGCTTGCCCTGTGCCCTTGGA
  ACTGTTAACCAAGGAGAAATCGTACTTCTCCACGGTGAAGATCATCTACACCACGGGCCACAGCATCTCC
  ATTGTAGCCCTCTGCGTGGCTATTGCCATCCTGGTTGCCCTCAGGAGGCTCCACTGCCCCAGGAACTACA
  TCCACACGCAGCTGTTTGCCACCTTCATCCTCAAGGCCAGTGCTGTGTTCTTGAAGGATGCCGCCATCTT
  CCAGGGGGATAGCACGGACCACTGCAGCATGTCCACTGTCCTGTGCAAGGTCTCTGTGGCCATCTCACAT
  TTGGCCACCATGACCAACTTCAGCTGGCTGTTGGCAGAAGCTGTCTACCTGAGCTGCCTGTTGGCCTCCA
  CATCCCCCAGGTCCAAACCAGCTTTTTGGTGGCTGGTCCTGGCTGGCTGGGGGCTCCCCGTGCTATGCAC
  TGGTACGTGGGTGGGCTGCAAACTGGCTTTTGAGGACACTGAGTGCTGGGACTTAGACAACAGCTCCCCC
  TGCTGGTGGATCATCAAAGGACCCATAGTCCTCTCTGTTGGGGTGAACTTTGGGCTGTTTCTCAATATAA
  TTTGTATCCTGCTGAGGAAGCTGGAGCCTGCACAAGGCGGCCTACACACACGGGCTCAGTACTGGCGGCT
  TTCCAAGTCAACACTTCTCCTTATACCGCTGTTTGGAATTCATTACATCATCTTCAACTTCCTGCCTGAC
  AGTGCTGGCCTTGACATCCGTGTACCCCTGGAGCTGGGGTTGGGGTCCTTTCAGGGCTTCATTGTTGCTG
  TCCTCTACTGCTTCCTCAATCAAGAGGTGAGGACAGAGATTTCACGCAAATGGTATGGCCACGACCCTGA
  ACTTCTGCCAGCTCGACGGACCTGCACTGAGTGGACCACACCTCCCCGATCGAGATTGAAGGTGCTGACC
  TCTGAGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224048 representing NM_001003685
  Red=Cloning site Green=Tags(s)
  
  MDGLMWATRILCLLSLCGVTLGHLHLECDFITQLRDDELACLQAAEGTNNTSLGCPGTWDGLLCWPPTGS
  GQWVSLPCPEFFSHFGSDTGFVKRDCTITGWSNPFPPYPVACPVPLELLTKEKSYFSTVKIIYTTGHSIS
  IVALCVAIAILVALRRLHCPRNYIHTQLFATFILKASAVFLKDAAIFQGDSTDHCSMSTVLCKVSVAISH
  LATMTNFSWLLAEAVYLSCLLASTSPRSKPAFWWLVLAGWGLPVLCTGTWVGCKLAFEDTECWDLDNSSP
  CWWIIKGPIVLSVGVNFGLFLNIICILLRKLEPAQGGLHTRAQYWRLSKSTLLLIPLFGIHYIIFNFLPD
  SAGLDIRVPLELGLGSFQGFIVAVLYCFLNQEVRTEISRKWYGHDPELLPARRTCTEWTTPPRSRLKVLT
  SEC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003685
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003685.1, NM_001003685.2, NM_001003685.3, NP_001003685.2
• RefSeq Size: 1598 bp
• RefSeq ORF: 1272 bp
• Locus ID: 14602
• Cytogenetics: 6 27.38 cM
• MW: 47.5 kDa
• Gene Summary: Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion. [UniProtKB/Swiss-Prot Function]