Cyp7a1 (NM_007824) Mouse Tagged ORF Clone

CAT#: MR224092

Cyp7a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 7, subfamily a, polypeptide 1 (Cyp7a1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp7a1
• Synonyms: CYPVII; CYPVIIc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224092 representing NM_007824
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAGCATTTCTTTGATCTGGGGGATTGCTGTGGTAGTGAGCTGTTGCATATGGTTTATCATTGGAA
  TAAGGAGAAGGAAAGTAGGTGAACCTCCTTTGGACAACGGGTTGATTCCATACCTGGGCTGTGCTCTGAA
  GTTCGGATCCAATCCTCTTGAATTCCTAAGAGCAAAGCAAAGGAAACATGGCCATGTTTTTACCTGCAAA
  CTGATGGGGAAATATGTCCACTTCATCACAAACTCCCTGTCATACCACAAAGTCTTATGTCACGGAAAAT
  ATTTTGACTGGAAAAAATTTCATTACACTACTTCTGCGAAGGCATTTGGACACAGAAGCATAGACCCAAG
  TGATGGAAATACCACGGAAAACATAAACAAGACTTTTAACAAAACCCTCCAGGGAGATGCTCTGTGTTCA
  CTCTCTGAAGCCATGATGCAAAACCTCCAATCTGTCATGAGACCTCCGGGCCTTCCTAAATCAAAGAGCG
  CTGTCTGGGTCACGGAAGGGATGTATGCCTTCTGCTACCGAGTGATGTTTGAAGCCGGATATCTAACGCT
  GTTTGGCAAAGATATTTCAAAGACAGACTCACAAAGAGCATTTATTCAAAACAACCTTGACAGCTTCAAA
  CAATTTGACCAAGTATTTCCGGCACTAGTGGCGGGCGTCCCTATTCACTTGTTCAAGACCGCACATAAAG
  CCCGGGAAAGGCTGGCTGAGAGCTTGAAGCACAAGAACCTGTACATGAGGGACCAGGTCTCTGAACTGAT
  CCGTCTACGCATGTTTCTCAACGATACACTCTCCACCTTTGATGACATGGAGAAGGCTAAGACGCACCTC
  GTGATCCTCTGGGCATCTCAAGCAAACACCATTCCTGCAACCTTCTGGAGCTTATTTCAAATGATCAGGA
  GCCCTGAAGCAATGAAAGCAGCCTCTGAAGAAGTGAATGGAGCATTACAGAGTGCTGGCCAAGAGCTCAG
  CTCTGGAGGGAATGCCATTTACTTGGATCAAGAGCAACTAAACAACCTGCCAGTACTAGATAGCATCATC
  AAGGAGGCTCTGCGGCTCTCCAGTGCATCCTTGAATATCCGGACAGCTAAGGAGGACTTCACTCTACACC
  TTGAGGATGGTTCCTATAACATTCGAAAAGATGACATCATAGCTCTTTACCCACAGTTAATGCACTTGGA
  TCCTGAAATCTACCCAGACCCTTTGACTTTTAAATACGACCGGTACCTTGATGAAAGTGGGAAAGCAAAG
  ACCACCTTCTATAGAAATGGAAACAAGCTGAAGTATTTCTACATGCCCTTTGGATCAGGAGCTACAATAT
  GTCCTGGAAGACTATTTGCTGTCCAAGAAATCAAGCAATTTTTGATTCTGATGCTGTCTTACTTTGAACT
  GGAGCTTGTAGAGAGCCACACCAAGTGTCCCCCTCTAGATCAGTCCCGGGCAGGCTTGGGAATTTTGCCA
  CCACTAAATGATATTGAGTTTAAATATAAACTGAAACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224092 representing NM_007824
  Red=Cloning site Green=Tags(s)
  
  MMSISLIWGIAVVVSCCIWFIIGIRRRKVGEPPLDNGLIPYLGCALKFGSNPLEFLRAKQRKHGHVFTCK
  LMGKYVHFITNSLSYHKVLCHGKYFDWKKFHYTTSAKAFGHRSIDPSDGNTTENINKTFNKTLQGDALCS
  LSEAMMQNLQSVMRPPGLPKSKSAVWVTEGMYAFCYRVMFEAGYLTLFGKDISKTDSQRAFIQNNLDSFK
  QFDQVFPALVAGVPIHLFKTAHKARERLAESLKHKNLYMRDQVSELIRLRMFLNDTLSTFDDMEKAKTHL
  VILWASQANTIPATFWSLFQMIRSPEAMKAASEEVNGALQSAGQELSSGGNAIYLDQEQLNNLPVLDSII
  KEALRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDIIALYPQLMHLDPEIYPDPLTFKYDRYLDESGKAK
  TTFYRNGNKLKYFYMPFGSGATICPGRLFAVQEIKQFLILMLSYFELELVESHTKCPPLDQSRAGLGILP
  PLNDIEFKYKLKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007824
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007824.1, NM_007824.2, NP_031850.2
• RefSeq Size: 4172
• RefSeq ORF: 1512
• Locus ID: 13122
• MW: 57.7 kDa