Rhot1 (NM_001163354) Mouse Tagged ORF Clone

CAT#: MR224107

Rhot1 (Myc-DDK-tagged) - Mouse ras homolog gene family, member T1 (Rhot1), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rhot1
• Synonyms: 2210403N23Rik; AA415293; AF244542; AI834919; Arht1; C430039G08Rik; Miro1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224107 representing NM_001163354
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTGCGGGCCGAGTGCGGCCCCTGCGAGCCTCCGACATGAAGAAAGACGTGAGGATCCTGCTGGTGG
  GAGAACCTCGAGTTGGGAAGACATCACTGATTATGTCGCTGGTCAGTGAAGAATTTCCGGAAGAGGTTCC
  TCCCCGAGCAGAAGAAATCACCATTCCAGCTGACGTCACCCCTGAAAGAGTTCCAACACACATTGTCGAT
  TATTCAGAAGCAGAACAGAGTGATGAACAACTTCATCAAGAAATATCACAGGCTAATGTTATCTGCATAG
  TTTATGCTGTTAACAACAAACATTCTATTGATAAGGTAACAAGCCGATGGATTCCTCTCATAAATGAAAG
  AACAGACAAAGACAGCAGGCTGCCTTTGATATTGGTTGGGAACAAATCTGATCTGGTGGAATATAGTAGT
  ATGGAGACCATCCTTCCAATTATGAACCAGTATACAGAGATAGAGACCTGTGTGGAGTGTTCAGCAAAAA
  ACCTAAAGAATATCTCAGAGCTGTTTTATTATGCACAGAAAGCTGTTCTTCATCCTACAGGGCCTCTATA
  CTGCCCAGAGGAGAAAGAGATGAAACCGGCTTGTATAAAGGCCCTCACTCGTATATTTAAAATATCTGAT
  CAGGATAACGACGGCACTCTGAATGATGCTGAGCTCAACTTCTTCCAGAGAATTTGTTTCAACACTCCTT
  TAGCTCCGCAAGCGCTGGAAGATGTCAAGAATGTGGTTAGAAAGCACTTAAGTGACGGTGTGGCTGACAG
  TGGGCTGACGCTCAGAGGTTTTCTCTTTTTACATACACTTTTTATCCAGAGGGGGAGGCATGAGACTACT
  TGGACTGTGCTTCGACGATTTGGTTATGACGATGACCTGGACCTGACGCCTGAGTATTTATTCCCCCTGC
  TAAAAATACCTCCTGATTGCACTACTGAATTAAATCATCATGCATATTTGTTTCTCCAGAGCACCTTTGA
  CAAACATGATTTGGACAGAGACTGTGCTTTGTCGCCTGATGAACTTAAAGATTTATTTCAAGTTTTTCCT
  TACATACCTTGGGGACCAGATGTAAATAACACAGTTTGCACAAATGAAAGAGGCTGGATAACCTACCAGG
  GGTTCCTCTCCCAGTGGACGCTCACCACCTACTTAGATGTGCAGCGTTGCCTGGAGTATTTGGGATATCT
  AGGCTATTCCATACTGACGGAACAAGAGTCTCAAGCTTCAGCCATTACAGTAACAAGGGATAAAAAGATT
  GACCTGCAGAAAAAACAGACTCAGAGAAATGTGTTCAGGTGTAATGTAATTGGAGTGAAAGGCTGTGGGA
  AAACTGGAGTTCTTCAGTCTCTCCTTGGAAGAAACCTAATGAGGCAGAAGAAAATTCGTGATGATCATAA
  GTCCTATTATGCAATTAACACTGTCTATGTATATGGACAAGAGAAATACTTGTTGCTGCACGATATCTCA
  GAGTCGGAATTTCTAACTGAGGCTGAGACCATTTGTGATGTTGTATGCCTGGTATATGATGTCACCAATC
  CCAAATCCTTTGAATACTGTGCCAGGATTTTTAAGCAACATTTTATGGACAGCAGAATTCCCTGCTTGAT
  TGTAGCTGCAAAGTCAGACCTGCATGAAGTCAAACAGGAACATAGCATCTCACCTACTGATTTCTGCAGG
  AAACACAAAATGCCTCCACCTCAAGCCTTCACTTGCAATACTGCTGATGCCCCCAGTAAGGATATCTTTG
  TTAAGTTGACAACAATGGCCATGTACCCAGAGGATCATTACAGAGGCAGCCTCTCCCGAGACATGGGCAG
  CACTGATAGAATAGAGAATTTGAGAAAAATCTGGGTCTTTCTAAAAACTGCTTTGCACGTGACACAAGCT
  GACCTCAAGAGCTCAACGTTCTGGCTTCGCGCAAGCTTTGGTGCTACGGTCTTTGCAGTCGTGGGCTTTG
  CTATGTACAGAGCACTACTGAAACAGCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224107 representing NM_001163354
  Red=Cloning site Green=Tags(s)
  
  MRAGRVRPLRASDMKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVD
  YSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSS
  METILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISD
  QDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHLSDGVADSGLTLRGFLFLHTLFIQRGRHETT
  WTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFQVFP
  YIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAITVTRDKKI
  DLQKKQTQRNVFRCNVIGVKGCGKTGVLQSLLGRNLMRQKKIRDDHKSYYAINTVYVYGQEKYLLLHDIS
  ESEFLTEAETICDVVCLVYDVTNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEHSISPTDFCR
  KHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPEDHYRGSLSRDMGSTDRIENLRKIWVFLKTALHVTQA
  DLKSSTFWLRASFGATVFAVVGFAMYRALLKQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163354
• ORF Size: 1989 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001163354.1, NP_001156826.1
• RefSeq Size: 4233
• RefSeq ORF: 1992
• Locus ID: 59040
• MW: 76.5 kDa