Malt1 (NM_172833) Mouse Tagged ORF Clone

CAT#: MR224110

Malt1 (Myc-DDK-tagged) - Mouse mucosa associated lymphoid tissue lymphoma translocation gene 1 (Malt1)

Product Images

Western validation with an anti-DDK antibody; L: Control HEK293 lysate R: Over-expression lysate

• 

  Coomassie blue staining of purified Malt1 protein (Cat# TP524110). The protein was produced from HEK293T cells transfected with Malt1 cDNA clone (Cat# MR224110) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Malt1
• Synonyms: A630046N12; D430033E09Rik; Pcasp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224110 representing NM_172833
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCTGTGGGGGCAGCCGCTGCAGGCCTCACCTCCCCTGGCCGTGCGGCAGCCCCCTACCGCGTCCT
  CGGGACCTTCGACCTCCCCTCCAGCCGGCGCAACCCTCAACCGCCTGCCCGAGCCGCTGCTGCGGAGGCT
  CAGCGAGAGCTTGGACCGCGCTCCGGAGGGCCGGGGCTGGCGGCAGCTGGCCGAGCTGGCGGGCAGTCGG
  GGACGCCTCCGGCTCAGTGGCCTGGACCTGGAACAGTGTTCTCTTAAAGTATTGGAGCCAGAGGGAAGCC
  CAAGTTTGTGTTTATTGAAACTAATGGGTGAAAAAGGATGTACAGTCACAGAACTGAGCGACTTCCTACA
  GGCTTTGGAGCACACTGAGGTTCTTCCGCTTCTCAACCCCCCAGGATTAAAGATCACTGTAAACCCAGAA
  TCCAAGGCAGTGTTGGCTGGACAGTTTGTAAAACTGTGCTGCCGGGCAACTGGACATCCTTTTGTACAAT
  ATCAATGGTTCAAAATGAATAAAGAGATCCCATATGGAAATTCATCAGAACTTGTTTTTAACACAGTGCA
  TGTAAAAGATGCAGGCTTTTATGTCTGCCGGGTTAACAACAGTTCCACTTTTGAATTTAGCCAGTGGTCA
  CAGCTGGATGTGTGTGATGTGGCAGAAGTAACAGACAGCTTCCAGGGAAGTATGGATGGCATCTCAGAAT
  CCAGGTTGCAAATCTGTGTTGAACCAAGGTCCCAAAGGCTGGTGCCAGGCAGCATGTTGTTGTTACAGTG
  TGTTGCTATTGGAAGTCCTATGCCTCACTACCAGTGGTTTAAAGATGAATCACCATTAACACATGAGACA
  AAAAAGCACTACACGGTTCCATATGTGGATATAGAACATGAAGGAACCTACTGGTGTCATGTATATAATG
  ACCGAGACAGTCAAGATAGCAAGAAGGCAGAAGTCACCATAGATGAATTAAACAATTTGGGGCATCCTGA
  TAATAAAGAACAAACTGGCCAGCCTTTGGCAAAGGACAAAGTCGCCCTTTTGATAGGGAATATGAGTTAC
  TGGGAGCATCCCAAGCTTAAAGCGCCTTTGGTGGATGTGTATGAATTGACCAACTTACTAAGACAACTAG
  ATTTCAAAGTTGTCTCACTGTTGGACCTTACTGAATATGAGATGTGTAACGCTGTGGATGAATTTTTACT
  ACTTTTAGACAAAGGAGTATATGGATTGTTATATTATGCAGGGCACGGTTATGAAAACTTTGGAAACAGT
  TTCATGGTCCCTGTTGATGCTCCAAATCCATATAGGTCTGAAAATTGCCTATGCGTACAAAACATACTGA
  AATTAATGCAAGAAAAGGAGACTGGCCTGAATGTGTTCCTGTTGGATATGTGTCGGAAAAGAAATGACTA
  CGATGATACCATTCCAATCTTGGACGCACTGAAAGTCACTGCCAACATTGTGTTTGGATATGCCACGTGC
  CAAGGAGCAGAAGCCTTTGAAATCCAGCATTCTGGGTTGGCAAATGGGATTTTTATGAAATTTTTAAAAG
  ATAGATTATTAGAAGACAAGAAAATTACTGTGTTACTGGATGAAGTTGCAGAAGATATGGGTAAATGTCA
  CCTTACCAAAGGCAGACAAGCTCTAGAGATTCGCAGCAGCTTGTCTGAAAAGAGAGCACTTACTGATCCA
  GTCCAGGGAGCACCTTGCTCTGCAGAGGCTCTGGTGCGCAATCTACAGTGGGCCAAGGCTCATGAACTTC
  CAGAAAGTATGTGCCTTAAATTTCAGTGTGGCGTTCACATTCAATTAGGATTTGCCGCAGAGTTCTCCAA
  TGTCATGATAATCTACACAAGTATAGTCCACAAACCACCTGAGATAATAATGTGTGACGCCTATGTTACT
  GATTTCCCTCTTGACCTAGATATTGATCCAAAACATGCAAACAAGGGGACTCCTGAAGAAACTGGCAGCT
  ACTTAGTATCAAAAGACCTTCCCAAGCACTGCCTCTACACTAGACTCAGCTCACTACAAAAATTAAAGGA
  ACATCTGATCTTCACAGTCTGTCTGTCCTATCAGTATTCAGGACTAGAAGACACTGTGGAGGAGAAGCAG
  GAGGTGAACGTGGGGAAGCCTCTTATTGCCAAGCTAGACATGCACCGAGGACTGGGCAGGAAGACTTGCT
  TCCAAGCTTGTCGTATGCCTGACGAGCCGTACCACAGCTCCACATCTACCTCAGCGGGAGCGGGGCATTT
  CCATTCCTCTCAGGACTCATTCCATGATGTGTACCATTCACATCTTGGCAATGCAGACAGTGGTATGCCA
  CCAGACAGGTGTCATTGCAGCCGGACTCCACACACATTCATTTCAAATTATCCCCCCCACCACTACTGCC
  AGTTTGGTAGAAGTAATGTGCCCGTAGAGACAACTGATGAAATGCCATTCAGTTTTTCTGACAGGCTTAT
  GATTTCTGAAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224110 representing NM_172833
  Red=Cloning site Green=Tags(s)
  
  MSLWGQPLQASPPLAVRQPPTASSGPSTSPPAGATLNRLPEPLLRRLSESLDRAPEGRGWRQLAELAGSR
  GRLRLSGLDLEQCSLKVLEPEGSPSLCLLKLMGEKGCTVTELSDFLQALEHTEVLPLLNPPGLKITVNPE
  SKAVLAGQFVKLCCRATGHPFVQYQWFKMNKEIPYGNSSELVFNTVHVKDAGFYVCRVNNSSTFEFSQWS
  QLDVCDVAEVTDSFQGSMDGISESRLQICVEPRSQRLVPGSMLLLQCVAIGSPMPHYQWFKDESPLTHET
  KKHYTVPYVDIEHEGTYWCHVYNDRDSQDSKKAEVTIDELNNLGHPDNKEQTGQPLAKDKVALLIGNMSY
  WEHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMCNAVDEFLLLLDKGVYGLLYYAGHGYENFGNS
  FMVPVDAPNPYRSENCLCVQNILKLMQEKETGLNVFLLDMCRKRNDYDDTIPILDALKVTANIVFGYATC
  QGAEAFEIQHSGLANGIFMKFLKDRLLEDKKITVLLDEVAEDMGKCHLTKGRQALEIRSSLSEKRALTDP
  VQGAPCSAEALVRNLQWAKAHELPESMCLKFQCGVHIQLGFAAEFSNVMIIYTSIVHKPPEIIMCDAYVT
  DFPLDLDIDPKHANKGTPEETGSYLVSKDLPKHCLYTRLSSLQKLKEHLIFTVCLSYQYSGLEDTVEEKQ
  EVNVGKPLIAKLDMHRGLGRKTCFQACRMPDEPYHSSTSTSAGAGHFHSSQDSFHDVYHSHLGNADSGMP
  PDRCHCSRTPHTFISNYPPHHYCQFGRSNVPVETTDEMPFSFSDRLMISEN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172833
• ORF Size: 2463 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172833.1, NM_172833.2, NP_766421.1
• RefSeq Size: 5008
• RefSeq ORF: 2466
• Locus ID: 240354
• MW: 92.5 kDa