Map3k8 (NM_007746) Mouse Tagged ORF Clone

CAT#: MR224251

Map3k8 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase 8 (Map3k8)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Map3k8
• Synonyms: c-COT; Cot; Cot/Tpl2; Est; Estf; Tpl-2; Tpl2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224251 representing NM_007746
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTACATGAGCACTGGAAGTGACGAGAAAGAAGAAATTGATTTATTAATTAAGCATTTAAACGTGT
  CAGAAGTCATAGACATAATGGAGAACCTTTATGCAAGTGAAGAGCCAGGAGTGTATGAGCCCAGTCTGAT
  GACCATGTATCCAGACAGCAATCAAAATGAGGAACGTTCGGAGTCACTGCTTCGGAGTGGCCAGGAGGTT
  CCCTGGCTGTCATCTGTCAGATATGGGACCGTGGAGGATCTGCTTGCATTTGCAAACCATGTCTCCAATA
  TGACAAAGCATTTTTATGGACGTCGACCACAAGAATGTGGAATTTTATTAAATATGGTAATCAGTCCCCA
  GAATGGCCGCTACCAAATCGATTCGGATGTTCTCCTTGTTCCTTGGAAGCTGACATACAGGAACATCGGC
  TCTGGTTTCGTTCCTCGGGGGGCCTTTGGAAAAGTATACTTAGCCCAAGACATGAAGACAAAGAAAAGAA
  TGGCGTGCAAACTGATCCCTATAGATCAGTTTAAGCCATCGGATGTGGAAATCCAGGCCTGTTTCCGGCA
  TGAGAACATTGCTGAGTTATATGGTGCCGTCCTATGGGGCGATACTGTCCATCTCTTTATGGAAGCCGGC
  GAGGGAGGGTCTGTTCTGGAGAAACTGGAGAGTTGTGGGCCCATGAGAGAATTTGAAATTATTTGGGTGA
  CGAAGCACATTCTCAAGGGACTTGATTTTCTGCACTCCAAGAAAGTGATCCACCATGATATTAAACCTAG
  CAACATTGTATTCATGTCTACAAAAGCTGTTTTGGTAGATTTTGGCCTGAGTGTTAAGATGACTGAAGAT
  GTCTATCTTCCCAAGGACCTCCGGGGAACAGAGATATACATGAGCCCAGAGGTGATCCTATGTCGGGGCC
  ATTCCACAAAAGCCGACATCTACAGCCTTGGAGCCACACTCATCCACATGCAGACAGGCACCCCACCCTG
  GGTGAAGCGCTACCCTCGATCAGCCTATCCCTCCTACCTATACATTATCCACAAGCAGGCACCTCCCCTG
  GAAGACATCGCTGGTGACTGCAGTCCAGGCATGAGGGAGCTGATAGAAGCTGCCCTGGAGAGGAACCCCA
  ACCACCGCCCCAAAGCAGCAGACCTACTGAAACATGAAGCCCTGAATCCCCCAAGAGAGGACCAGCCACG
  ATGTCAGAGTCTGGACTCTGCCCTCTTTGAACGGAAGAGGCTGCTGAGCAGGAAGGAACTACAACTTCCT
  GAGAACATTGCTGATTCATCATGCACAGGCAGCACCGAAGAGTCTGAAGTGCTCAGGAGACAGCGTTCCC
  TCTACATTGACCTCGGAGCTCTGGCTGGCTACTTCAATATTGTTCGTGGGCCACCAACCCTGGAATACGG
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224251 representing NM_007746
  Red=Cloning site Green=Tags(s)
  
  MEYMSTGSDEKEEIDLLIKHLNVSEVIDIMENLYASEEPGVYEPSLMTMYPDSNQNEERSESLLRSGQEV
  PWLSSVRYGTVEDLLAFANHVSNMTKHFYGRRPQECGILLNMVISPQNGRYQIDSDVLLVPWKLTYRNIG
  SGFVPRGAFGKVYLAQDMKTKKRMACKLIPIDQFKPSDVEIQACFRHENIAELYGAVLWGDTVHLFMEAG
  EGGSVLEKLESCGPMREFEIIWVTKHILKGLDFLHSKKVIHHDIKPSNIVFMSTKAVLVDFGLSVKMTED
  VYLPKDLRGTEIYMSPEVILCRGHSTKADIYSLGATLIHMQTGTPPWVKRYPRSAYPSYLYIIHKQAPPL
  EDIAGDCSPGMRELIEAALERNPNHRPKAADLLKHEALNPPREDQPRCQSLDSALFERKRLLSRKELQLP
  ENIADSSCTGSTEESEVLRRQRSLYIDLGALAGYFNIVRGPPTLEYG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007746
• ORF Size: 1401 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007746.1, NM_007746.2, NP_031772.1
• RefSeq Size: 2507
• RefSeq ORF: 1404
• Locus ID: 26410
• MW: 53.4 kDa