Phgdh (NM_016966) Mouse Tagged ORF Clone

CAT#: MR224471

Phgdh (Myc-DDK-tagged) - Mouse 3-phosphoglycerate dehydrogenase (Phgdh)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Phgdh
• Synonyms: 3-PGDH; 3PGDH; 4930479N23; A10; PGAD; PGD; PGDH; SERA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224471 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTCGCAAATCTGCGCAAAGTACTCATCAGTGACAGCCTGGACCCCTGCTGCCGGAAGATCCTGC
  AAGATGGAGGGCTGCAGGTGGTGGAGAAGCAGAACTTGAGCAAGGAGGAGCTGATAGCTGAACTCCAGGA
  CTGTGAAGGCCTCATTGTCCGGTCTGCTACTAAGGTCACTGCTGATGTCATCAATGCAGCAGAGAAGCTC
  CAGGTGGTGGGCAGGGCTGGCACAGGTGTGGACAATGTGGATCTGGAGGCTGCCACGAGGAAGGGCATCC
  TAGTCATGAACACCCCCAACGGGAACAGCCTCAGTGCTGCGGAGCTCACCTGTGGGATGATCATGTGCCT
  GGCCAGGCAGATTCCCCAGGCAACAGCTTCGATGAAAGATGGCAAATGGGACCGGAAGAAGTTCATGGGG
  ACAGAACTGAACGGGAAGACACTGGGAATTCTTGGCCTCGGCAGAATTGGAAGAGAGGTGGCCACCCGAA
  TGCAATCCTTTGGAATGAAGACTGTAGGCTATGACCCCATCATCTCTCCTGAAGTCGCCGCCTCCTTTGG
  TGTTCAGCAGCTGCCGCTGGAGGAGATCTGGCCTCTCTGTGACTTTATAACTGTCCATACCCCACTCCTG
  CCCTCTACCACAGGCTTGCTGAATGACAGCACCTTTGCTCAGTGCAAGAAAGGTGTGCGAGTAGTGAACT
  GTGCTCGAGGAGGCATTGTGGATGAAGGTGCCCTGCTGCGTGCCCTGCAGTCTGGTCAGTGTGCTGGGGC
  TGCACTGGATGTGTTTACAGAAGAGCCACCACGGGACCGGGCCTTAGTGGACCACGAGAATGTCATCAGC
  TGTCCCCACCTGGGTGCCAGCACCAAGGAAGCCCAGAGCCGCTGTGGGGAGGAAATCGCAGTCCAGTTTG
  TGGACATGGTGAAGGGGAAATCTCTAACAGGCGTTGTGAACGCCCAGGCCCTCACCAGTGCCTTCTCTCC
  ACACACCAAGCCTTGGATTGGTCTGGCAGAAGCAATGGGCACGCTGATGCACGCCTGGGCTGGCTCCCCT
  AAAGGGACCATCCAGGTGGTTACACAAGGAACATCTCTGAAGAATGCTGGGACCTGCCTGAGCCCTGCAG
  TCATTGTCGGCCTTCTGAGAGAAGCATCTAAGCAGGCAGACGTGAACTTGGTGAACGCTAAGCTACTGGT
  GAAAGAGGCTGGCCTCAATGTCACCACCTCCCACAACCCTGGGGTTCCAGGGGAGCAGGGTAGCGGGGAA
  TGCCTCCTGACTGTGGCCCTGGCAGGTGCTCCCTACCAAGCCGTGGGCTTGGTCCAGGGCACCACACCAA
  TGCTGCAGATGCTCAACGGAGCTGTCTTCAGACCAGAGGTGCCACTACGCAGGGGCCAACCCCTGCTCGT
  ATTCCGGGCTCAGCCCTCCGACCCTGGGATGCTGCCCACTATGATTGGCCTCCTGGCAGAGGCGGGTGTA
  CAGCTGCTGTCCTACCAAACCTCCATGGTGTCTGACGGAGAGCCCTGGCACGTCATGGGCCTGTCCTCCC
  TGCTGCCCAGCCTGGAAACATGGAAGCAGCATGTATTGGAGGCTTTCCAGTTCTGCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224471 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEGLIVRSATKVTADVINAAEKL
  QVVGRAGTGVDNVDLEAATRKGILVMNTPNGNSLSAAELTCGMIMCLARQIPQATASMKDGKWDRKKFMG
  TELNGKTLGILGLGRIGREVATRMQSFGMKTVGYDPIISPEVAASFGVQQLPLEEIWPLCDFITVHTPLL
  PSTTGLLNDSTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVIS
  CPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEAMGTLMHAWAGSP
  KGTIQVVTQGTSLKNAGTCLSPAVIVGLLREASKQADVNLVNAKLLVKEAGLNVTTSHNPGVPGEQGSGE
  CLLTVALAGAPYQAVGLVQGTTPMLQMLNGAVFRPEVPLRRGQPLLVFRAQPSDPGMLPTMIGLLAEAGV
  QLLSYQTSMVSDGEPWHVMGLSSLLPSLETWKQHVLEAFQFCF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016966
• ORF Size: 1602 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016966.1, NM_016966.2, NM_016966.3, NP_058662.2
• RefSeq Size: 1871
• RefSeq ORF: 1602
• Locus ID: 236539
• MW: 56.6 kDa