Carmil2 (NM_001033320) Mouse Tagged ORF Clone

CAT#: MR224472

Rltpr (Myc-DDK-tagged) - Mouse RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing (Rltpr)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Carmil2
• Synonyms: CG1399-PB; D130029J02Rik; Gm585; Rltpr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224472 representing NM_001033320
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCTGCCACTGAATGACGTAACCCAGGCTCATCGCAGCCGGCCAGAACTCACAACTCGAGCGGTCC
  ATCAGATCCAAGCCTGTCTCTGGAGGAACAACCAAGTAGACTCTACTTCGGACCTCAAGCCCTGCCTTCA
  GCCCTTAGGTCTGATTTCAGACCACTCAGAGCAGGAGGTGAACGAGCTATGCCAGTCAGTACAGGAGCAT
  ATGGAGCTGCTGGGCTGTGGGGCTGGGCCCCAGGGTGAGGTTGCCGTGCACCAGGCTGAGGACGCCATCC
  AGAATGCCAACTTCTCTCTCAGTATCCTCCCCATTCTCTATGAGGCTGGGAGATCCCCAAGCCACCACTG
  GCAGCTGCAGCAGAAGCTAGAGAGCCTCCTGGGTCAGGTGGGCGAGATCTGCCGCCAGGACATCCAGGAC
  TTCACTCAGACCACCCTGGATACCACAAGGAGCCTCTGCCCACAGATGTTGCAGACACCTGGCTGGAGGA
  AGCAGCTAGAGGGAGTTCTGGTGGGCTCCGGGGGCCTCCCAGAGCTGCTTCCGGAACATCTGCTGCAAGA
  TGCCTTCTCTAGGCTGAGGGACATGCGCCTGTCAATCACTGGGACCCTAGCAGAGAGCATTGTGGCTCAG
  GCTCTTGCAGGTCTTCATGCAGCCCGAGATCGACTGGTGGAGAGGCTAACTCAGCAGGCACCAGTGACCA
  TGGCCCCTGCTGTACCACCACTGGGTGGAAATGAGCTCAGCCCCCTTGAGACTGGGGGATTGGAAGAGCT
  TTTCTTTCCCACGGAGAAGGAAGAGGAGAGAGAAAAGGATGAGAGTTCTTCATGGAAATGGCTTGAGCCT
  AGTAACTGTTTTCACCTGGTCTCCTCCCTTCATGGTGCTGCTGAGGAAGCGGAACGGGACCCCGAGCTGG
  CAGCTCCGGGGGAAGATGCAGAGCCGCAGGCTGGGCCGTCTGCACGCGGCTCTCCAAGCCCCGCCGCCCC
  GGGGCCACCCGCCGGCCCTCTGCCTCGCATGGACCTGCCACCCGCCGGGCAACCCCTACGCCACCCAACC
  CGAGCCCGACCGAGACCACGTCGCCAGCACCACCACCGCCCGCCGCCGGGGGGCCCCCAGGTGCCCCCAG
  CCCTGCTTCAAGAAGGAAATGGGCTCACTGCTCGCGTGGATGAGGGTGTGGAGGAGTTCTTCTCCAAAAG
  GCTGATCCAGCAGGATCACTTCTGGGCCCCAGAGGAGGATCCAGCCACTGAGGGTGGTGCCACTCCTGTC
  CCCCGCACACTTCGAAAGAAGCTGGGTACGCTCTTTGCCTTTAAGAAACCTCGTTCAACAAGGGGTCCGC
  GACCTGACCTGGAGACCAGCCCTGGAGCAGCAGCTCGAGCCAGAAAATCCACACTTGGGGATCTCCTGCG
  ACCACCGGCCCGTCCAGGCCGTGGTGAGGAACCTGGAGGGGCGGAAGGCGGCACCAGCAGCCCTGACCCT
  GCTCGCAGAAATCGGCCTCGGTACACGCGGGAAAGCAAGGCCTACTCCATGATCCTGCTACCTGCTGAGG
  AGGAAGCAGCCGTGGGTACCAGACCTGATAAGAGGCGGCCTCTGGAACGGGGAGACACAGAACTGGCTCC
  ATCTTTTGAGCAGCGGGTACAAGTGATGCTACAGAGGATCGGCGTGAGCCGGGCCAGTGGGGGTGCCGAG
  AGCAAGAGGAAGCAAAGTAAAGACGGCGAGATCAAGAAGGCAGGCTCTGATGGTGACATTATGGACAGTT
  CCACAGAGACCCCTCCCATCTCAATCAAGTCCCGTACCCACTCTGTGTCTGCTGATCCCTCATGCAGACC
  TGGGCCAGGAGGCCAGGGGCCTGAGTCTGCCACCTGGAAGACATTGGGACAGCAGCTGAATGCAGAGCTC
  AGAGGCCGTGGTTGGGGCCAACAGGATGGTCCAGGGCCCCCCTCCCCATGTCCCAGCCCAAGTCCCCGAA
  GAACCAGCCCCGCCCCAGACATCCTGAGTCTCCCAGAGGACCCCTGCTTGGGCCCTAGGAATGAAGAACG
  GCCCCTCCGGCTGCAGCGCTCCCCTGTCCTCAAGCGTAGGCCGAAGCTCGAGGCACCCCCATCCCCAAGC
  TTAGGCTCTGGCCTTGGATCCAAGCCTCTTCCTCCGTACCCCACAGAACCCTCCAGCCCTGAGCGGAGCC
  CTCCCTCCCCAGCCACAGACCAAAGAGGCGGCGGCCCCAACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224472 representing NM_001033320
  Red=Cloning site Green=Tags(s)
  
  MPLPLNDVTQAHRSRPELTTRAVHQIQACLWRNNQVDSTSDLKPCLQPLGLISDHSEQEVNELCQSVQEH
  MELLGCGAGPQGEVAVHQAEDAIQNANFSLSILPILYEAGRSPSHHWQLQQKLESLLGQVGEICRQDIQD
  FTQTTLDTTRSLCPQMLQTPGWRKQLEGVLVGSGGLPELLPEHLLQDAFSRLRDMRLSITGTLAESIVAQ
  ALAGLHAARDRLVERLTQQAPVTMAPAVPPLGGNELSPLETGGLEELFFPTEKEEEREKDESSSWKWLEP
  SNCFHLVSSLHGAAEEAERDPELAAPGEDAEPQAGPSARGSPSPAAPGPPAGPLPRMDLPPAGQPLRHPT
  RARPRPRRQHHHRPPPGGPQVPPALLQEGNGLTARVDEGVEEFFSKRLIQQDHFWAPEEDPATEGGATPV
  PRTLRKKLGTLFAFKKPRSTRGPRPDLETSPGAAARARKSTLGDLLRPPARPGRGEEPGGAEGGTSSPDP
  ARRNRPRYTRESKAYSMILLPAEEEAAVGTRPDKRRPLERGDTELAPSFEQRVQVMLQRIGVSRASGGAE
  SKRKQSKDGEIKKAGSDGDIMDSSTETPPISIKSRTHSVSADPSCRPGPGGQGPESATWKTLGQQLNAEL
  RGRGWGQQDGPGPPSPCPSPSPRRTSPAPDILSLPEDPCLGPRNEERPLRLQRSPVLKRRPKLEAPPSPS
  LGSGLGSKPLPPYPTEPSSPERSPPSPATDQRGGGPNP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033320
• ORF Size: 2214 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033320.1, NM_001033320.2, NP_001028492.1
• RefSeq Size: 2504 bp
• RefSeq ORF: 2217 bp
• Locus ID: 234695
• Cytogenetics: 8 D3
• MW: 80.2 kDa