Ube3a (NM_011668) Mouse Tagged ORF Clone

CAT#: MR224630

Ube3a (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3A (Ube3a), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ube3a
• Synonyms: 4732496B02; 5830462N02Rik; A130086L21Rik; Hpve6a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224630 representing NM_011668
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACAGCTTGTAAAAGATCACCAGGAGAATCCCAGTCTGAGGACATTGAAGCTAGCCGAATGAAGC
  GAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCATCAGTTAACTGAGGGCTGTGGAAATGAGGCCTG
  CACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAACAATGCAGCAGCTATTAAAGCC
  CTTGAGCTTTATAAAATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTT
  ACCTTGAGAACTCAAAAGGTGCATCTAACAACTCAGAGATAAAAATGAACAAGAAGGAAGGAAAAGATTT
  TAAAGATGTGATTTACCTAACTGAAGAGAAAGTATATGAAATTTATGAATTTTGTAGAGAGAGTGAGGAT
  TATTCCCCTTTAATTCGTGTAATTGGAAGAATATTTTCTAGTGCTGAGGCACTGGTTCTGAGCTTTCGGA
  AAGTCAAACAGCACACAAAGGAGGAATTGAAATCTCTTCAAGAAAAGGATGAAGACAAGGATGAAGATGA
  AAAGGAAAAAGCTGCATGTTCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCTTCAAGGATGGGT
  GATAGTTCACAGGGAGACAACAATGTACAAAAATTAGGTCCTGATGATGTGACTGTGGATATTGATGCTA
  TTAGAAGGGTCTACAGCAGTTTGCTCGCTAATGAAAAATTAGAAACTGCCTTCCTGAATGCACTTGTATA
  TCTGTCACCTAACGTGGAATGTGATTTGACATATCATAATGTGTATACTCGAGATCCTAATTATCTCAAT
  TTGTTCATTATTGTAATGGAGAATAGTAATCTCCACAGTCCTGAATATCTGGAAATGGCGTTGCCATTAT
  TTTGCAAAGCTATGTGTAAGCTACCCCTTGAAGCTCAAGGAAAACTGATTAGGCTGTGGTCTAAATACAG
  TGCTGACCAGATTCGGAGAATGATGGAAACATTTCAGCAACTTATTACCTACAAAGTCATAAGCAATGAA
  TTTAATAGCCGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCAAAGTGTTTGAAAATGGTTT
  ACTATGCAAATGTAGTGGGAGGGGATGTGGACACAAATCATAATGAGGAAGATGATGAAGAACCCATACC
  TGAGTCCAGCGAATTAACACTTCAGGAGCTTCTGGGAGATGAAAGAAGAAATAAGAAAGGTCCTCGAGTG
  GATCCACTAGAAACCGAACTTGGCGTTAAAACTCTAGACTGTCGAAAACCACTTATCTCCTTTGAAGAAT
  TCATTAATGAACCACTGAATGATGTTCTAGAAATGGACAAAGATTATACCTTTTTCAAAGTTGAAACAGA
  GAACAAATTCTCTTTTATGACATGTCCCTTTATATTGAATGCTGTCACAAAGAATCTGGGATTATATTAT
  GACAATAGAATTCGCATGTACAGTGAAAGAAGAATCACTGTTCTTTACAGCCTAGTTCAAGGACAGCAGT
  TGAATCCGTATTTGAGACTCAAAGTCAGACGTGACCATATTATAGATGATGCACTGGTCCGGCTAGAGAT
  GATTGCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACAAGGAGTA
  GATGAGGGAGGCGTTTCCAAAGAGTTTTTTCAGTTGGTTGTGGAGGAAATTTTTAATCCAGATATTGGTA
  TGTTCACATATGATGAAGCTACGAAATTATTTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAGTT
  TACTCTGATTGGCATAGTCCTGGGTCTGGCTATTTACAATAATTGTATACTGGATGTCCATTTTCCCATG
  GTTGTATACAGGAAGCTAATGGGGAAAAAAGGAACCTTTCGTGACTTGGGAGACTCTCACCCAGTTTTAT
  ATCAGAGTTTAAAGGATTTATTGGAATATGAAGGGAGTGTGGAAGATGATATGATGATCACTTTCCAGAT
  ATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAAGAAAATGGTGATAAAATTCCAATT
  ACAAATGAAAACAGGAAGGAATTTGTCAATCTCTATTCAGACTACATTCTCAATAAATCTGTAGAAAAAC
  AATTCAAGGCATTTCGCAGAGGTTTTCATATGGTGACTAATGAATCGCCCTTAAAATACTTATTCAGACC
  AGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCTAGATTTCCAGGCACTAGAAGAAACTACAGAG
  TATGACGGTGGCTATACGAGGGAATCTGTTGTGATTAGGGAGTTCTGGGAAATTGTTCATTCGTTTACAG
  ATGAACAGAAAAGACTCTTTCTGCAGTTTACAACAGGCACAGACAGAGCACCTGTTGGAGGACTAGGAAA
  ATTGAAGATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCTACATCTCATACTTGCTTTAAT
  GTCCTTTTACTTCCGGAATATTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGCCATCACATATG
  CCAAAGGATTTGGCATGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224630 representing NM_011668
  Red=Cloning site Green=Tags(s)
  
  MATACKRSPGESQSEDIEASRMKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKA
  LELYKINAKLCDPHPSKKGASSAYLENSKGASNNSEIKMNKKEGKDFKDVIYLTEEKVYEIYEFCRESED
  YSPLIRVIGRIFSSAEALVLSFRKVKQHTKEELKSLQEKDEDKDEDEKEKAACSAAAMEEDSEASSSRMG
  DSSQGDNNVQKLGPDDVTVDIDAIRRVYSSLLANEKLETAFLNALVYLSPNVECDLTYHNVYTRDPNYLN
  LFIIVMENSNLHSPEYLEMALPLFCKAMCKLPLEAQGKLIRLWSKYSADQIRRMMETFQQLITYKVISNE
  FNSRNLVNDDDAIVAASKCLKMVYYANVVGGDVDTNHNEEDDEEPIPESSELTLQELLGDERRNKKGPRV
  DPLETELGVKTLDCRKPLISFEEFINEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYY
  DNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGV
  DEGGVSKEFFQLVVEEIFNPDIGMFTYDEATKLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPM
  VVYRKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGSVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPI
  TNENRKEFVNLYSDYILNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTE
  YDGGYTRESVVIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFN
  VLLLPEYSSKEKLKERLLKAITYAKGFGML
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011668
• ORF Size: 2610 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011668.2, NP_035798.2
• RefSeq Size: 5097
• RefSeq ORF: 2613
• Locus ID: 22215
• MW: 100.3 kDa