Treh (NM_021481) Mouse Tagged ORF Clone

CAT#: MR224712

Treh (Myc-DDK-tagged) - Mouse trehalase (brush-border membrane glycoprotein) (Treh)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Treh
• Synonyms: 2210412M19Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224712 representing NM_021481
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTGGGAGCTGCACCTGCTGCTTCTGCTGGGGCTGGGACTTAGGTCCCAGGAGGCCCTGCCACCAC
  CCTGTGAGAGCCAGATCTACTGCCATGGAGAGCTCCTGCACCAAGTTCAGATGGCCCAGCTCTACCAAGA
  TGACAAGCAGTTTGTGGATATGTCACTGGCCACATCTCCAGATGAAGTCCTGCAGAAGTTCAGTGAGCTG
  GCCACAGTCCACAACCACAGCATCCCCAAGGAACAGCTTCAGGAATTTGTCCAGAGTCACTTCCAGCCCG
  TGGGGCAGGAGCTGCAGTCCTGGACCCCTGAGGACTGGAAGGACAGCCCTCAGTTCCTGCAGAAGATCTC
  GGATGCTAATCTGCGTGTCTGGGCGGAGGAGCTACACAAGATCTGGAAAAAGCTGGGAAAGAAGATGAAA
  GCAGAAGTCCTCAGCTACCCCGAGAGGTCCTCCCTAATCTACTCAAAGCACCCCTTCATTGTGCCCGGGG
  GGCGCTTTGTTGAATTCTACTACTGGGACTCGTACTGGGTGATGGAAGGCCTGCTTCTTTCTGAGATGGC
  CTCAACAGTGAAGGGTATGCTGCAAAACTTTCTGGATCTGGTGAAGACCTACGGACATATCCCCAACGGT
  GGACGCATATATTACCTGCAACGGAGCCAGCCCCCACTCCTGACTCTCATGATGGATCGATATGTAGCTC
  ATACCAAGGATGTCGCCTTCCTTCAGGAGAATATTGGGACTCTAGCCTCTGAACTGGACTTCTGGACTGT
  GAACAGGACTGTCTCTGTAGTCTCAGGAGGACAAAGCTATGTCTTAAATCGCTACTATGTCCCTTATGGG
  GGACCCAGGCCAGAGTCCTACAGGAAAGACGCAGAATTGGCAAACTCTGTGCCAGAAGGGGACCGAGAGA
  CTCTGTGGGCTGAGCTCAAGGCTGGGGCTGAGTCTGGCTGGGACTTCTCTTCACGCTGGCTTGTTGGAGG
  CCCAGACCCTGATTTGCTCAGCAGCATCCGAACCAGCAAAATGGTACCCGCTGATCTGAACGCGTTCCTG
  TGCCAAGCAGAGGAACTGATGAGTAACTTCTACTCCAGACTAGGGAACGACACAGAGGCCACAAAGTACA
  GGAACCTGCGGGCCCAGCGCTTGGCCGCCATGGAAGCTGTCCTGTGGGACGAGCAGAAGGGTGCCTGGTT
  TGACTATGACTTGGAAAAGGGGAAGAAGAACCTGGAGTTTTATCCCTCCAACCTCTCCCCACTTTGGGCT
  GGCTGCTTCTCAGACCCTAGTGTTGCTGACAAGGCTCTGAAGTACTTGGAGGACAGCAAGATCTTGACCT
  ACCAATATGGAATCCCAACCTCTCTTCGTAACACAGGCCAGCAGTGGGACTTCCCCAATGCCTGGGCCCC
  ACTGCAGGACCTGGTCATTAGAGGTTTGGCCAAGTCAGCTTCCCCCCGGACTCAGGAGGTGGCTTTCCAG
  CTGGCCCAGAATTGGATCAAAACCAACTTCAAAGTCTACTCCCAAAAGTCAGCGATGTTTGAGAAGTATG
  ACATCAGCAACGGTGGACATCCAGGTGGAGGAGGGGAGTATGAAGTTCAGGAAGGATTTGGCTGGACAAA
  CGGATTGGCCCTGATGCTTCTGGATCGCTATGGTGACCAGTTGACTTCAGGGACCCAGTTAGCTTCCCTG
  GGACCCCACTGCCTAGTGGCTGCCCTTCTTCTCAGTCTTCTGCTACAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224712 representing NM_021481
  Red=Cloning site Green=Tags(s)
  
  MTWELHLLLLLGLGLRSQEALPPPCESQIYCHGELLHQVQMAQLYQDDKQFVDMSLATSPDEVLQKFSEL
  ATVHNHSIPKEQLQEFVQSHFQPVGQELQSWTPEDWKDSPQFLQKISDANLRVWAEELHKIWKKLGKKMK
  AEVLSYPERSSLIYSKHPFIVPGGRFVEFYYWDSYWVMEGLLLSEMASTVKGMLQNFLDLVKTYGHIPNG
  GRIYYLQRSQPPLLTLMMDRYVAHTKDVAFLQENIGTLASELDFWTVNRTVSVVSGGQSYVLNRYYVPYG
  GPRPESYRKDAELANSVPEGDRETLWAELKAGAESGWDFSSRWLVGGPDPDLLSSIRTSKMVPADLNAFL
  CQAEELMSNFYSRLGNDTEATKYRNLRAQRLAAMEAVLWDEQKGAWFDYDLEKGKKNLEFYPSNLSPLWA
  GCFSDPSVADKALKYLEDSKILTYQYGIPTSLRNTGQQWDFPNAWAPLQDLVIRGLAKSASPRTQEVAFQ
  LAQNWIKTNFKVYSQKSAMFEKYDISNGGHPGGGGEYEVQEGFGWTNGLALMLLDRYGDQLTSGTQLASL
  GPHCLVAALLLSLLLQ
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021481
• ORF Size: 1728 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021481.1, NM_021481.2, NM_021481.3, NP_067456.1
• RefSeq Size: 2053
• RefSeq ORF: 1731
• Locus ID: 58866
• MW: 65.4 kDa
• Gene Summary: This gene belongs to the alpha-glucosidase family, whose members encode enzymes that carry out hydrolysis of alpha-glucoside bonds of a variety of carbohydrates. The enzyme encoded by this gene uses the disaccharide trehalose as a highly specific substrate and converts it into two glucose molecules. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2013]