Asah2 (NM_018830) Mouse Tagged ORF Clone

CAT#: MR224900

Asah2 (Myc-DDK-tagged) - Mouse N-acylsphingosine amidohydrolase 2 (Asah2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asah2
• Synonyms: AI585898
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224900 representing NM_018830
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAGCGAACCTTCTCCACCTTGGAGGCATTCCTCATTTTCCTTCTGGTAATAATGACAGTCATCA
  CAGTGGCCCTTCTCACCCTCTTGTTTGTTACCAGTGGGACCATTGAAAACCACAAAGATTCAGGAAATCA
  CTGGTTTTCAACCACTCTGGGCTCCACGACAACCCAGCCCCCTCCAATTACACAGACTCCAAACTTCCCT
  TCATTTCGGAACTTCAGTGGCTACTACATTGGCGTTGGGAGAGCAGATTGCACAGGACAAGTGTCAGATA
  TCAATTTGATGGGCTATGGCAAAAATGGCCAGAATGCACGGGGTCTCCTCACCAGGCTGTTCAGCCGTGC
  TTTTATCTTGGCGGATCCAGATGGGTCAAATCGAATGGCATTTGTGAGCGTGGAACTATGTATGATTTCC
  CAACGACTGAGGTTGGAGGTCCTGAAGAGACTAGAGAGTAAATATGGCTCTCTGTATCGAAGAGACAATG
  TTATCCTGAGTGCCATTCACACACACTCTGGCCCAGCAGGGTTTTTCCAATATACACTCTATATACTCGC
  CAGCGAGGGATTCAGCAACCGGACCTTTCAGTACATAGTCTCTGGGATCATGAAGAGCATTGATATAGCT
  CACACAAATCTTAAACCAGGCAAAATCTTTATCAACAAAGGAAATGTTGCTAATGTGCAGATCAACCGAA
  GCCCCTCCTCTTACCTTCTGAATCCACAGTCAGAGAGAGCAAGGTATTCTTCAAACACAGACAAGGAAAT
  GCTGGTCTTGAAACTGGTGGATTTGAATGGAGAAGACTTGGGTCTTATCAGCTGGTTTGCCATCCACCCC
  GTGAGCATGAACAATAGCAACCACTTTGTGAATAGTGACAATATGGGCTATGCGGCTTACCTTTTTGAGC
  AAGAAAAGAACAAAGGCTATCTGCCTGGACAGGGACCGTTTGTAGCAGGCTTTGCTTCATCAAATCTCGG
  AGACGTGTCACCCAACATTCTTGGCCCGCATTGTGTCAACACAGGGGAGTCTTGTGACAACGACAAGAGC
  ACCTGTCCCAACGGTGGGCCTAGCATGTGCATGGCCAGCGGACCTGGACAAGACATGTTTGAGAGCACAC
  ACATTATAGGACGGATCATCTATCAGAAGGCCAAGGAGCTGTATGCCTCTGCCTCCCAGGAGGTGACCGG
  CCCAGTGCTTGCAGCTCACCAGTGGGTGAACATGACAGATGTGAGCGTCCAGCTCAATGCCACACACACA
  GTGAAGACGTGTAAACCTGCCCTGGGCTACAGTTTTGCCGCAGGCACAATTGATGGAGTTTCGGGCCTCA
  ATATTACACAGGGAACTACGGAAGGGGATCCATTCTGGGACACTCTTCGGGACCAGCTCTTGGGAAAACC
  ATCTGAAGAGATTGTAGAGTGTCAGAAACCCAAACCAATCCTGCTTCACAGTGGAGAGCTGACGATACCA
  CATCCTTGGCAACCAGATATTGTTGATGTTCAGATTGTTACCGTTGGGTCCTTGGCCATAGCTGCTATCC
  CTGGGGAATTAACAACCATGTCGGGACGAAGATTTCGTGAGGCAATTAAAAAAGAATTTGCACTTTATGG
  GATGAAGGATATGACCGTTGTTATCGCAGGTCTAAGCAATGTTTATACACATTACATTACCACATATGAA
  GAATACCAGGCTCAGCGGTACGAGGCAGCATCTACAATCTATGGACCACACACCCTGTCTGCATACATCC
  AACTCTTCAGAGACCTTGCTAAGGCAATTGCTACGGACACAGTAGCCAACATGAGCAGTGGTCCCGAGCC
  TCCATTCTTCAAAAATCTAATAGCTTCACTTATTCCTAATATTGCGGATAGAGCACCAATTGGCAAACAT
  TTTGGGGATGTCTTGCAGCCAGCAAAACCTGAATACAGAGTGGGAGAAGTGGTTGAAGTTATATTTGTAG
  GCGCTAACCCAAAGAATTCAGCAGAGAACCAGACCCATCAAACCTTCCTCACTGTGGAGAAATACGAGGA
  CTCTGTAGCTGACTGGCAGATAATGTATAACGATGCCTCCTGGGAGACGAGGTTTTATTGGCACAAAGGA
  ATACTGGGTCTGAGCAATGCAACAATATACTGGCATATTCCAGATACTGCCTACCCTGGAATCTACAGAA
  TAAGATATTTTGGACACAATCGGAAGCAGGAACTTCTGAAACCCGCTGTCATACTAGCATTTGAAGGAAT
  TTCTTCTCCTTTTGAAGTTGTCACTACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224900 representing NM_018830
  Red=Cloning site Green=Tags(s)
  
  MAKRTFSTLEAFLIFLLVIMTVITVALLTLLFVTSGTIENHKDSGNHWFSTTLGSTTTQPPPITQTPNFP
  SFRNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMIS
  QRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIA
  HTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHP
  VSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKS
  TCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHT
  VKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIP
  HPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGMKDMTVVIAGLSNVYTHYITTYE
  EYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKH
  FGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYWHKG
  ILGLSNATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSPFEVVTT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018830
• ORF Size: 2268 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018830.1, NP_061300.1
• RefSeq Size: 4835
• RefSeq ORF: 2271
• Locus ID: 54447
• MW: 84 kDa