Ccnt1 (NM_009833) Mouse Tagged ORF Clone

CAT#: MR224911

Ccnt1 (Myc-DDK-tagged) - Mouse cyclin T1 (Ccnt1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccnt1
• Synonyms: 2810478G24Rik; AI115585; CycT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224911 representing NM_009833
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGAGAGAGGAAGAACAACAACAAGCGGTGGTATTTTACTCGCGAGCAGCTGGAGAACAGCCCGT
  CGCGTCGCTTTGGCGTGGACTCCGATAAAGAACTCTCTTACCGCCAGCAGGCGGCCAATCTCCTCCAGGA
  CATGGGACAACGTCTGAACGTCTCACAACTGACGATCAATACTGCTATAGTATACATGCACCGATTCTAC
  ATGATTCAGTCTTTTACACAGTTTCATCGATATTCCATGGCTCCAGCAGCCTTGTTTCTAGCAGCAAAAG
  TAGAAGAACAACCAAAGAAATTGGAGCATGTCATCAAGGTGGCACACACTTGCCTGCACCCACAGGAGTC
  ACTTCCTGACACGAGGAGTGAGGCTTACCTACAACAAGTTCAAGATCTGGTGATTTTAGAGAGTATAATT
  TTGCAGACCTTAGGGTTTGAACTAACAATTGATCACCCACATACACATGTGGTAAAGTGCACCCAGCTTG
  TTCGAGCAAGCAAGGACTTAGCACAGACATCTTACTTCATGGCAACCAACAGCCTGCATTTGACCACATT
  TAGCCTGCAGTACACACCTCCTGTGGTGGCCTGTGTCTGCATCCATCTGGCTTGCAAGTGGTCCAACTGG
  GAGATCCCAGTCTCAACTGACGGGAAGCACTGGTGGGAGTATGTTGACGCCACTGTGACCTTGGAACTGT
  TAGATGAATTGACACATGAATTTCTACAGATTCTAGAGAAAACTCCAAGCAGGCTGAAACGTATTCGGAA
  TTGGAGGGCGTACCAGGCTGCCATGAAAACCAAGCCAGATGACCGAGGAGCAGATGAAAACACGTCGGAG
  CAGACAATCCTCAATATGATTTCTCAGACCTCTTCAGACACAACTATCGCAGGATTAATGAGCATGTCAA
  CTGCGTCTACAAGTGCAGTGCCTTCCCTGCCTTCCTCTGAGGAGTCATCGAGCAGTCTAACTAGTGTGGA
  TATGTTGCAGGGCGAGCGGTGGCTGTCTTCCCAACCTCCGTTTAAACTCGAAGCTGCCCAGGGTCATCGG
  ACTAGTGAGAGTTTAGCACTTATTGGCGTTGATCATTCCTTGCAACAGGATGGTTCCAGTGCGTTTGGTT
  CCCAGAAGCAGGCGAGCAAGAGTGTGCCATCTGCTAAGGTTTCACTTAAGGAGTACCGTGCAAAGCACGC
  GGAAGAGTTGGCTGCTCAGAAGAGGCAGCTGGAGAACATGGAGGCCAATGTGAAGTCACAGTATGCGTAT
  GCTGCCCAGAATCTCCTGTCTCACGACAGCCATTCTTCAGTTATTCTCAAAATGCCCATTGAGAGCTCAG
  AAAACCCTGAACGTCCTTTTCTGGATAAGGCTGACAAATCAGCTCTGAAAATGAGACTCCCTGTGGCCAG
  TGGGGATAAAGCAGTTTCTTCCAAGCCAGAGGAGATAAAGATGCGCATTAAAGTTCACTCTGCAGGGGAT
  AAGCACAATTCCATAGAAGACAGTGTCACCAAGAGCCGCGAGCACAAGGAGAAGCAGAGGACTCACCCGT
  CTAACCACCACCACCATCACAACCACCACTCACACAGGCACTCTCACTTACAGCTGCCAGCCGGCCCCGT
  GAGCAAACGCCCAAGTGATCCAAAACACAGCAGCCAGACAAGCACCTTAGCACACAAAACCTACAGCTTG
  TCCAGTACTTTGTCTTCTTCCAGCTCTACTCGTAAAAGGGGTCCCCCGGAAGAGACTGGGGCAGCAGTAT
  TTGATCATCCAGCCAAGATTGCCAAGAGTACTAAATCTTCCTTGAATTTCCCCTTCCCCCCCCTTCCTAC
  AATGACCCAGTTGCCTGGGCATAGCTCAGACACAAGTGGCCTGCCCTTTTCACAGCCTAGCTGTAAAACT
  CGAGTTCCTCATATGAAACTGGATAAAGGCCCTCCTGGGGCTAATGGTCACAACGCAACTCAGTCAATAG
  ATTATCAAGACACTGTGAATATGCTCCACTCCCTGCTTAGTGCCCAGGGGGTTCAGCCCACACAGGCCCC
  TGCATTTGAATTTGTCCACTCTTATGGTGAATATATGAATCCCAGAGCTGGTGCCATCTCCTCCAGATCT
  GGCACTACAGACAAACCTCGGCCACCACCCCTCCCATCAGAACCCCCTCCACCACTTCCACCTCTTCCTA
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224911 representing NM_009833
  Red=Cloning site Green=Tags(s)
  
  MEGERKNNNKRWYFTREQLENSPSRRFGVDSDKELSYRQQAANLLQDMGQRLNVSQLTINTAIVYMHRFY
  MIQSFTQFHRYSMAPAALFLAAKVEEQPKKLEHVIKVAHTCLHPQESLPDTRSEAYLQQVQDLVILESII
  LQTLGFELTIDHPHTHVVKCTQLVRASKDLAQTSYFMATNSLHLTTFSLQYTPPVVACVCIHLACKWSNW
  EIPVSTDGKHWWEYVDATVTLELLDELTHEFLQILEKTPSRLKRIRNWRAYQAAMKTKPDDRGADENTSE
  QTILNMISQTSSDTTIAGLMSMSTASTSAVPSLPSSEESSSSLTSVDMLQGERWLSSQPPFKLEAAQGHR
  TSESLALIGVDHSLQQDGSSAFGSQKQASKSVPSAKVSLKEYRAKHAEELAAQKRQLENMEANVKSQYAY
  AAQNLLSHDSHSSVILKMPIESSENPERPFLDKADKSALKMRLPVASGDKAVSSKPEEIKMRIKVHSAGD
  KHNSIEDSVTKSREHKEKQRTHPSNHHHHHNHHSHRHSHLQLPAGPVSKRPSDPKHSSQTSTLAHKTYSL
  SSTLSSSSSTRKRGPPEETGAAVFDHPAKIAKSTKSSLNFPFPPLPTMTQLPGHSSDTSGLPFSQPSCKT
  RVPHMKLDKGPPGANGHNATQSIDYQDTVNMLHSLLSAQGVQPTQAPAFEFVHSYGEYMNPRAGAISSRS
  GTTDKPRPPPLPSEPPPPLPPLPK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009833
• ORF Size: 2172 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009833.1, NP_033963.1
• RefSeq Size: 2175
• RefSeq ORF: 2175
• Locus ID: 12455
• MW: 80.6 kDa
• Gene Summary: Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). [UniProtKB/Swiss-Prot Function]