Brsk2 (NM_001009929) Mouse Tagged ORF Clone

CAT#: MR224920

Brsk2 (Myc-DDK-tagged) - Mouse BR serine/threonine kinase 2 (Brsk2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Brsk2
• Synonyms: 4833424K13Rik; SAD-A; SADA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224920 representing NM_001009929
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACATCGACGGGGAAGGACGGCGGCGGGGCGCAGCACGCGCAGTATGTGGGGCCCTACCGGCTGGAGA
  AGACGCTGGGCAAGGGGCAGACAGGCTTGGTGAAGCTGGGAATCCACTGTGTCACTTGCCAGAAGGTCGC
  CATCAAAATCGTGAACCGTGAGAAGCTCAGTGAGTCGGTGCTGATGAAGGTGGAGCGAGAGATTGCCATC
  TTGAAGCTCATCGAGCATCCACATGTACTGAAGCTGCATGACGTCTATGAAAACAAAAAATATTTATACC
  TGGTGCTAGAACATGTGTCTGGGGGAGAGCTGTTCGACTACCTGGTGAAGAAGGGCCGGCTGACCCCCAA
  GGAGGCCCGCAAGTTCTTCCGGCAGATCATCTCTGCACTGGACTTCTGTCACAGCCACTCCATATGCCAT
  AGAGACTTGAAGCCAGAGAACCTGCTGCTAGATGAGAGGAACAACATCCGTATTGCAGACTTTGGCATGG
  CATCCCTGCAGGTGGGAGACAGCCTGCTGGAGACCAGCTGCGGATCTCCACACTATGCCTGTCCGGAAGT
  GATTCGGGGCGAGAAGTATGATGGCCGCAAGGCAGATGTGTGGAGCTGTGGTGTGATCCTGTTCGCCTTG
  CTGGTGGGGGCTCTGCCTTTTGATGATGACAACCTGCGGCAGTTGCTGGAGAAGGTCAAGCGTGGTGTGT
  TCCACATGCCACACTTTATCCCACCAGACTGCCAGAGTCTCCTGCGTGGCATGATTGAGGTGGATGCAGC
  TCGGCGCCTCACGCTAGAGCACATTCAGAAACACATATGGTATATAGGTGGCAAGAATGAGCCAGAGCCC
  GAACAGCCCATCCCACGCAAGGTGCAGATCCGCTCACTACCCAGCTTGGAAGACATTGACCCTGATGTGT
  TGGACAGCATGCACTCACTGGGCTGCTTCCGAGACCGCAACAAGCTGCTGCAGGATCTGCTATCTGAGGA
  GGAGAATCAGGAAAAGATGATTTATTTCCTCCTCCTGGATCGGAAAGAACGGTATCCAAGCCATGAGGAT
  GAGGACCTGCCCCCCAGGAATGAGATAGACCCTCCCCGGAAGCGTGTGGATTCCCCGATGCTGAACCGGC
  ATGGCAAGCGGCGACCTGAGCGCAAGTCCATGGAAGTGCTCAGTGTGACAGATGGTGGCTCCCCAGTGCC
  TGCACGGAGAGCCATTGAGATGGCCCAGCATGGCCAGAGATCTCGATCCATCAGTGGTGCGTCCTCAGGC
  CTTTCTACAAGTCCACTCAGCAGTCCTCGGGTGACCCCTCACCCCTCACCAAGGGGTAGTCCCCTTCCTA
  CCCCCAAAGGGACGCCTGTCCACACGCCAAAGGAGAGCCCAGCTGGCACACCCAACCCCACACCACCATC
  CAGCCCTAGTGTTGGAGGAGTGCCCTGGCGGACACGACTGAACTCCATCAAGAACAGCTTCCTGGGCTCA
  CCTCGATTCCACCGCCGGAAACTCCAAGTTCCCACGCCAGAGGAGATGTCCAACCTGACCCCAGAATCCT
  CTCCAGAGCTGGCCAAGAAATCGTGGTTCGGGAACTTCATCAACCTGGAGAAGGAGGAGCAGATCTTTGT
  GGTGATCAAGGACAAGCCCCTGAGCTCCATCAAGGCTGACATCGTTCATGCCTTCCTGTCGATCCCCAGC
  CTCAGCCACAGCGTTATTTCCCAGACAAGCTTCAGGGCTGAATACAAGGCCACAGGGGGCCCAGCAGTGT
  TCCAGAAGCCGGTCAAGTTCCAGGTGGACATCACCTACACTGAGGGCGGAGAGGCCCAGAAGGAGAATGG
  CATCTACTCAGTCACATTCACTTTACTCTCAGGCCCCAGTCGCCGCTTCAAGAGGGTGGTGGAGACCATC
  CAGGCCCAGCTGTTAAGCACCCATGACCAGCCATCAGCCCAGCACCTGTCAGAACCACCCCCGCCAGCGC
  CAGGACTAAGCTGGGGTGCTGGGCTTAAGGGCCAGAAGGTGGCCACCAGCTACGAGAGTAGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224920 representing NM_001009929
  Red=Cloning site Green=Tags(s)
  
  MTSTGKDGGGAQHAQYVGPYRLEKTLGKGQTGLVKLGIHCVTCQKVAIKIVNREKLSESVLMKVEREIAI
  LKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALDFCHSHSICH
  RDLKPENLLLDERNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILFAL
  LVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWYIGGKNEPEP
  EQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMIYFLLLDRKERYPSHED
  EDLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDGGSPVPARRAIEMAQHGQRSRSISGASSG
  LSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPNPTPPSSPSVGGVPWRTRLNSIKNSFLGS
  PRFHRRKLQVPTPEEMSNLTPESSPELAKKSWFGNFINLEKEEQIFVVIKDKPLSSIKADIVHAFLSIPS
  LSHSVISQTSFRAEYKATGGPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSRRFKRVVETI
  QAQLLSTHDQPSAQHLSEPPPPAPGLSWGAGLKGQKVATSYESSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001009929
• ORF Size: 2025 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009929.1, NM_001009929.2, NM_001009929.3, NP_001009929.1
• RefSeq Size: 2028 bp
• RefSeq ORF: 2028 bp
• Locus ID: 75770
• Cytogenetics: 7 F5
• MW: 75.8 kDa