Abcg5 (NM_031884) Mouse Tagged ORF Clone

CAT#: MR224940

Abcg5 (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family G (WHITE), member 5 (Abcg5)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcg5
• Synonyms: cmp; sterolin-1; trac
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR224940 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGAGCTGCCCTTTCTGAGTCCAGAGGGAGCCAGAGGGCCTCACATCAACAGAGGGTCTCTGAGCT
  CCCTGGAGCAAGGTTCGGTCACGGGCACAGAGGCTCGGCACAGCTTAGGTGTCCTGCATGTGTCCTACAG
  CGTCAGCAACCGTGTCGGGCCTTGGTGGAACATCAAATCATGCCAGCAGAAGTGGGACAGGCAAATCCTC
  AAAGATGTCTCCTTGTACATCGAGAGTGGCCAGATTATGTGCATCTTAGGCAGCTCAGGCTCAGGGAAGA
  CCACGCTGCTGGACGCCATCTCCGGGAGGCTGCGGCGCACTGGGACCCTGGAAGGGGAGGTGTTTGTGAA
  TGGCTGCGAGCTGCGCAGGGACCAGTTCCAAGACTGCTTCTCCTACGTCCTGCAGAGCGACGTTTTTCTG
  AGCAGCCTCACTGTGCGCGAGACGTTGCGATACACAGCGATGCTGGCCCTCTGCCGCAGCTCCGCGGACT
  TCTACAACAAGAAGGTAGAGGCAGTCATGACAGAGCTGAGCCTGAGCCACGTGGCGGACCAAATGATTGG
  CAGCTATAATTTTGGGGGAATTTCCAGTGGCGAGCGGCGCCGAGTTTCCATCGCAGCCCAACTCCTTCAG
  GACCCCAAGGTCATGATGCTAGATGAGCCAACCACAGGACTGGACTGCATGACTGCAAATCAAATTGTCC
  TTCTCTTGGCTGAGCTGGCTCGCAGGGACCGAATTGTGATTGTCACCATCCACCAGCCTCGCTCTGAGCT
  CTTCCAACACTTCGACAAAATTGCCATCCTGACTTACGGAGAGTTGGTGTTCTGTGGCACCCCAGAGGAG
  ATGCTTGGCTTCTTCAATAACTGTGGTTACCCCTGTCCTGAACATTCCAATCCCTTTGATTTTTACATGG
  ACTTGACATCAGTGGACACCCAAAGCAGAGAGCGGGAAATAGAAACGTACAAGCGAGTACAGATGCTGGA
  ATGTGCCTTCAAGGAATCTGACATCTATCACAAAATTCTGGAGAACATTGAAAGAGCACGATACCTGAAA
  ACCTTACCCACGGTTCCTTTCAAAACAAAAGATCCTCCTGGGATGTTCGGCAAGCTTGGTGTCCTGCTGA
  GGCGAGTAACAAGAAACTTAATGAGGAATAAGCAGGCAGTGATTATGCGTCTCGTTCAGAATCTGATCAT
  GGGCCTCTTCCTCATTTTCTACCTTCTCCGCGTCCAGAACAACACGCTAAAGGGCGCTGTGCAGGACCGC
  GTGGGGCTGCTCTATCAGCTTGTGGGTGCCACCCCATACACCGGCATGCTCAATGCTGTGAATCTGTTTC
  CCATGCTGAGAGCCGTCAGCGACCAGGAGAGTCAGGATGGCCTGTATCATAAGTGGCAGATGCTGCTCGC
  CTACGTGCTACACGTCCTCCCCTTCAGCGTCATCGCCACGGTCATTTTCAGCAGTGTGTGTTATTGGACT
  CTGGGCTTGTATCCTGAAGTTGCCAGATTTGGATATTTCTCTGCTGCTCTTTTGGCCCCTCACTTAATTG
  GAGAATTTCTAACACTTGTGCTGCTTGGTATAGTCCAAAACCCTAATATTGTCAACAGTATAGTGGCTCT
  GCTCAGCATCTCTGGGCTGCTTATTGGATCTGGATTTATCAGAAACATACAAGAAATGCCCATTCCTTTA
  AAAATCCTGGGTTATTTTACATTCCAAAAATACTGTTGTGAGATTCTCGTGGTCAATGAGTTTTACGGCC
  TGAACTTCACTTGTGGTGGATCCAACACCTCTATGCTAAATCACCCGATGTGCGCCATCACCCAAGGGGT
  CCAGTTCATCGAGAAAACCTGCCCAGGTGCTACATCCAGATTCACGGCAAACTTCCTCATCTTATATGGG
  TTTATCCCAGCTCTGGTCATCCTAGGAATAGTGATTTTTAAAGTCAGGGACTACCTGATTAGCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR224940 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGELPFLSPEGARGPHINRGSLSSLEQGSVTGTEARHSLGVLHVSYSVSNRVGPWWNIKSCQQKWDRQIL
  KDVSLYIESGQIMCILGSSGSGKTTLLDAISGRLRRTGTLEGEVFVNGCELRRDQFQDCFSYVLQSDVFL
  SSLTVRETLRYTAMLALCRSSADFYNKKVEAVMTELSLSHVADQMIGSYNFGGISSGERRRVSIAAQLLQ
  DPKVMMLDEPTTGLDCMTANQIVLLLAELARRDRIVIVTIHQPRSELFQHFDKIAILTYGELVFCGTPEE
  MLGFFNNCGYPCPEHSNPFDFYMDLTSVDTQSREREIETYKRVQMLECAFKESDIYHKILENIERARYLK
  TLPTVPFKTKDPPGMFGKLGVLLRRVTRNLMRNKQAVIMRLVQNLIMGLFLIFYLLRVQNNTLKGAVQDR
  VGLLYQLVGATPYTGMLNAVNLFPMLRAVSDQESQDGLYHKWQMLLAYVLHVLPFSVIATVIFSSVCYWT
  LGLYPEVARFGYFSAALLAPHLIGEFLTLVLLGIVQNPNIVNSIVALLSISGLLIGSGFIRNIQEMPIPL
  KILGYFTFQKYCCEILVVNEFYGLNFTCGGSNTSMLNHPMCAITQGVQFIEKTCPGATSRFTANFLILYG
  FIPALVILGIVIFKVRDYLISR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031884
• ORF Size: 1959 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031884.1, NM_031884.2, NP_114090.1
• RefSeq Size: 2266
• RefSeq ORF: 1959
• Locus ID: 27409
• MW: 73.2 kDa
• Gene Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily, and functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. Disruption of this gene in mice results in thrombocytopenia, prolonged bleeding times, anemia, leukopenia, infertility, shortened life span and cardiomyopathy. Mice lacking this gene show symptoms of sitosterolemia. [provided by RefSeq, Nov 2015]