Cpvl (NM_027749) Mouse Tagged ORF Clone

CAT#: MR225047

Cpvl (Myc-DDK-tagged) - Mouse carboxypeptidase, vitellogenic-like (Cpvl)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cpvl
• Synonyms: 4933436L16Rik; HVLP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225047 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCGTGCCAAGTGGAAGATGGTTGTTTCACTGATCTTGTTTATGGTCAGCCCTGGTGATGGACTGT
  TTCATGCAGTATACCGAAGTATCCTTGTTTCCCAGTCCTTCAAGGGGGATGCAGGACAGCCTCTTTTTCT
  TAGCCCATACATCAAAAACGGGAAGATCAAGGAGGGGCAAAGGAAGAGCATGGTCAGTCCGTTCCCTGGA
  ATGAACGACAAGAGTTATGCCGGCTACATCACGGTGAACCAGACTTACAACAGCAACCTCTTCTTCTGGT
  TCTTTCCGGCTCGGATGCAGCCTGAGGATGCCCCAGTAGTCCTCTGGCTTCAGGGTGGACCTGGAGGTTC
  ATCCATGTTTGGACTCTTTGTAGAACACGGGCCTTATATTATCACAAGTAACATGACCGTTGTGGCCAGA
  GACTTCCCTTGGACCTTTACCCTTTCCATGCTCTACATTGATAATCCGGTGGGTACAGGCTTCAGCTTCA
  CCGATCATTTCCAGGGATACGCCACCAGCGAGGATGATGTAGCCCAAGACTTGTACAGTGCCCTGATTCA
  GTTCTTCACGTTGTTTCCCGAGTATGCAAAGAATGATTTTTACGTCACTGGCGAGTCTTATGCAGGGAAA
  TATGTTCCGGCCCTAGCACACTATATCCACTCCCTCAACCCCGTGCGGAAGTTCAAGATTCGCCTAAAAG
  GAATTGCCATTGGAGATGCATACACTGATCCTGAATCGATTATAGGAGGGTATGCGGCATTCCTATATGA
  AATTGGCTTGTTGGATGAACAGCAGCAGAAGTACTTCCAGAAGCAGTGTAGCAAGTGCGTAAAATACATC
  AAAGAGCAGGAGTGGATGAAGGCCTTTGAAATCCTGGATAAGCTGCTAGATGGAGATGTAACAACTGGTT
  CATCTTTCTTCCAGAATGTGACAGGATGTACCAATTACTACAACATTTTACAGTGCACGGAACCCAAGGA
  GCAAAGTTACTTTGCAAAATTCTTGACGCTCCCCCAAGTGAGACAAGCCATCCACGTGGGAAACCAGAAC
  TTCAGTGACGGTGCTGAGGTTGAGAAGCACCTGCGAGAGGACACAGTGAAGTCGGTGAAGCCCTGGCTGT
  CTGAGATCATGAATTACTACAAGGTTCTCATCTACAATGGCCAACTGGACATCATCGTGGCGGCTGCCCT
  GACAGAGCGCTCCTTGATGGCCATGGACTGGAAGGGATCCCGAGCATACAGAAGGGCACGTCGAAAGGTC
  TGGAAGATCTTCAAATCGGATAATGAAGTGGCTGGTTATGTGAGGCGTGTGGGCAAATTCCATCAGGTAA
  TCGTCCGAGGTGGAGGACACATTTTGCCCTATGACCAGCCAATGAGATCTTTTGATATGATCAATCGCTT
  CATCTATGACAGAGGATGGGAACCTTATAATTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225047 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVRAKWKMVVSLILFMVSPGDGLFHAVYRSILVSQSFKGDAGQPLFLSPYIKNGKIKEGQRKSMVSPFPG
  MNDKSYAGYITVNQTYNSNLFFWFFPARMQPEDAPVVLWLQGGPGGSSMFGLFVEHGPYIITSNMTVVAR
  DFPWTFTLSMLYIDNPVGTGFSFTDHFQGYATSEDDVAQDLYSALIQFFTLFPEYAKNDFYVTGESYAGK
  YVPALAHYIHSLNPVRKFKIRLKGIAIGDAYTDPESIIGGYAAFLYEIGLLDEQQQKYFQKQCSKCVKYI
  KEQEWMKAFEILDKLLDGDVTTGSSFFQNVTGCTNYYNILQCTEPKEQSYFAKFLTLPQVRQAIHVGNQN
  FSDGAEVEKHLREDTVKSVKPWLSEIMNYYKVLIYNGQLDIIVAAALTERSLMAMDWKGSRAYRRARRKV
  WKIFKSDNEVAGYVRRVGKFHQVIVRGGGHILPYDQPMRSFDMINRFIYDRGWEPYNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027749
• ORF Size: 1437 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_027749.1, NM_027749.2, NP_082025.1
• RefSeq Size: 1708
• RefSeq ORF: 1437
• Locus ID: 71287
• MW: 54.8 kDa
• Gene Summary: This gene encodes a member of the serine carboxypeptidase family of proteases that cleave amino acids from the C-terminus of a protein substrate. The human ortholog of this gene, where it was first characterized, was found to be upregulated during the maturation of monocytes to macrophages. The encoded protein may be involved in antigen processing, digestion of phagocytosed proteins in the lysosome and lamellipodium formation. Disruption of this gene in mice was found to cause embryonic lethality. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]