Eif2ak4 (NM_013719) Mouse Tagged ORF Clone

CAT#: MR225162

Eif2ak4 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif2ak4
• Synonyms: 2610011M03; GCN2; MGCN2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225162 representing NM_013719
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGGGCCGCGGGGCCTCTGGACGCGGCCGGGCTGAGCCACAGGAGAGCTATTCGCAGCGACAGG
  ACCACGAGCTGCAGGCCCTGGAGGCCATTTACGGCTCGGACTTCCAGGATCTGCGGCCGGATGCGCGCGG
  ACGGGTGAGAGAGCCTCCTGAAATCAACTTAGTTCTTTACCCTCAGGGCCTAGCTGGTGAAGAAGTATAC
  GTGCAAGTGGAACTGAGGGTTAAATGCCCACCTACATACCCAGATGTAGTTCCCGAAATAGACTTAAAAA
  ATGCTAAAGGCCTGTCGAATGAAAGTGTTAATTTGTTAAAATCACACCTGGAAGAACTGGCCAAAAAGCA
  GTGTGGAGAGGTGATGATATTTGAACTGGCACACCATGTGCAGTCGTTTCTCAGCGAGCATAACAAGCCC
  CCACCAAAGTCTTTCCATGAAGAAATGCTGGAAAGGCAGGCTCAGGAGAAGCAGCAGAGGTTGCTGGAGG
  CCAGGCGGAAGGAGGAGCAGGAGCAACGTGAAATCCTCCACGAGATTCAGAGAAGGAAAGAGGAGATCAA
  GGAAGAGAAGAAAAGGAAGGAAATGGCTAAGCAGGAACGTTTGGAAATCACTAGCTTGACAAACCAGGAC
  TATGCCTCTAAGAGAGACCCAGCAGGGCACAGGGCAGCTGCCATCCTCCATGGAGGCTCTCCTGACTTTG
  TAGGAAATGGTAAAGCCCGGACATACTCCTCAGGAAGGTCCAGGCGAGAACGTCAGTATTCTGTGTGTAG
  TGGTGAACCCTCTCCTGGCTCGTGTGACATCCTCCATTTCTCTGTGGGTAGCCCTGATCAGCTCATGGTG
  CACAAAGGGAGATGTGTTGGCAGTGATGAGCAGCTGGGAAAGGTGGTATACAATGCTTTGGAAACAGCCA
  CGGGCAGCTTTGTCTTGCTGCATGAGTGGGTCCTTCAATGGCAGAAGATGGGCCCTTGCCTTACCAGTCA
  AGAGAAAGAGAAGATTGACAAGTGCAAAAGGCAGATTCAAGGAGCAGAAACAGAATTCAGCTCCCTAGTG
  AAACTGAGCCATCCAAATATCGTCCGCTACTTTGCGATGAACTCCAGAGAGGAGGAGGACTCCATTGTGA
  TCGACATTCTGGCAGAGCACGTCAGTGGCATCTCTCTGGCTACACACCTGAGCCACTCGGGCCCAGTCCC
  CGCCCACCAGCTGCGCAAGTACACAGCCCAGCTCCTGGCAGGCCTGGATTACCTACACAGCAACTCCGTG
  GTGCACAAGGTTCTGAGCGCGTCCAGTGTCTTGGTAGATGCCGAGGGCACTGTCAAGATAACAGACTACA
  GCATCTCTAAGCGTCTGGCAGACATTTGCAAGGAGGATGTATTTGAGCAAGCTCGAGTTCGTTTTAGTGA
  CAGTGCCCTGCCTTATAAAACAGGAAAGAAAGGGGATGTGTGGCGGCTCGGCCTCCTGCTGTTGTCTCTC
  AGCCAAGGACAGGAGTGTGGGGAGTATCCGGTGACCATCCCCAGTGACCTGCCAGCTGACTTCCAAGACT
  TCCTGAAGAAGTGTGTCTGCCTGGACGACAAGGAAAGATGGAGCCCTCAGCAGCTGCTGAAACACAGCTT
  TATAAACCCTCAACCAAAGCTGCCTTTGGTGGAGCAGAGTCCTGAAGACTCTGGGGGACAAGACTACATT
  GAGACCGTCATTCCCAGCAACCAGCTGCCCAGCGCTGCGTTCTTCAGTGAGACACAGAAACAGTTCTCCC
  GGTACTTCATTGAGTTTGAAGAACTACAACTCCTAGGGAAAGGAGCTTTTGGAGCTGTCATCAAGGTGCA
  AAACAAGCTTGATGGCTGCTGCTATGCTGTGAAGCGCATCCCTATCAACCCTGCAAGCAGACACTTCCGC
  CGGATCAAGGGCGAGGTGACACTGCTATCGCGCCTGCACCATGAGAACATTGTGCGCTACTACAACGCCT
  GGATCGAGCGGCATGAGCGCCCAGCGGTGCCAGGGACACCGCCCCCAGACTGCACACCCCAGGCCCAGGA
  CAGCCCAGCCACCTGCGGGAAAACATCAGGCGACACTGAAGAACTGGGCAGCGTGGAGGCTGCAGCACCG
  CCACCCATCCTCAGCAGCTCGGTGGAGTGGAGCACATCTGCAGAGCGTTCTACCAGCACCCGCTTCCCAG
  TCACGGGCCAGGATTCCAGCAGCGATGAGGAGGACGAGGACGAGCGCGATGGCGTCTTCTCCCAGTCCTT
  TCTACCTGCTTCAGATTCTGACAGTGACATCATCTTTGACAATGAAGATGAAAACAGTAAAAGTCAAAAT
  CAGGATGAAGACTGCAATCAAAAGGACGGTAGCCATGAGATTGAGCCTTCAGTGACAGCTGAGGCTGTGC
  ACTACCTTTATATCCAGATGGAGTACTGCGAGAAGAGCACGCTGCGGGACACCATTGACCAGGGGCTGTT
  CCGAGACACCAGCCGGCTCTGGAGGCTTTTCCGAGAGATTCTGGATGGATTAGCTTATATCCATGAGAAA
  GGAATGATTCATCGCGACTTGAAGCCTGTCAATATTTTTTTGGATTCTGATGACCATGTGAAAATAGGTG
  ACTTTGGCCTGGCAACAGACCATCTGGCCTTCACTGCTGAAGGTAAACAGGACGACCAGGCAGGTGACGG
  AGTGATTAAGTCAGACCCTTCAGGCCATTTGACTGGCATGGTTGGTACTGCTCTGTATGTAAGCCCTGAG
  GTCCAAGGAAGCACCAAGTCTGCATACAACCAGAAAGTGGATCTCTTCAGCCTGGGAATTATCTTCTTTG
  AGATGTCTTATCACCCGATGGTGACTGCCTCAGAAAGAATTTTCGTTCTCAACCAACTTCGAGATCCCAC
  ATCGCCCAAGTTTCCAGACGACTTCGATGATGGAGAACATACAAAGCAGAAATCTGTCATCTCCTGGCTG
  TTGAACCATGATCCAGCTAAACGGCCCACGGCCATGGAACTGCTCAAGAGTGAGCTGCTCCCCCCGCCGC
  AGATGGAGGAGTCCGAGCTGCATGAAGTGCTGCACCACACGCTGGCCAACATAGATGGGAAGGCCTACCG
  CACCATGATGAGTCAGATCTTCTGCCAGCACATCTCCCCCGCTATAGACTACACCTACGACAGTGACATC
  CTGAAGGGCAACTTCTTGATTCGTACAGCCAAGATCCAGCAGCTTGTATGTGAAACCATCGTCCGCGTCT
  TTAAAAGGCATGGCGCTGTCCAGCTGTGCACCCCACTGCTGCTTCCCCGAAACAGGCAAATATATGAGCA
  CAACGAAGCGGCTTTGTTCATGGATCACAGCGGCATGCTGGTGATGCTTCCGTTCGACCTGCGGGTCCCT
  TTTGCAAGATATGTGGCAAGAAATAACATACTGAATTTAAAACGATACTGCATAGAGCGGGTGTTCAGAC
  CTCGGAAGTTAGACCGATTTCATCCCAAAGAACTCCTGGAATGTGCATTTGATATCGTCACTTCTACCAC
  CAACAGCTCTCTGCCCACTGCCGAAACCATCTACACCATCTATGAAATAATCCAGGAGTTTCCTGCACTT
  CAGGAAAGGAATTACAGCATTTACTTGAACCATACGATGCTTCTGAAGGCAATACTCCTACACTGTGGGA
  TCCCAGAGGACAAGCTCAGCCAAGTCTACGTCATTCTGTATGATGCTGTGACAGAGAAGCTGACTAGGAG
  AGAAGTGGAAGCTAAATTCTGTAACCTGTCCTTGTCTTCTAATAGCCTGTGTAGACTCTACAAGTTCATT
  GAGCAGAAGGGGGATTTGCAAGACTTAACGCCAACCATCAACTCACTAATAAAACAGAAAACAGGCGTTG
  CTCAGTTGGTGAAGTATAGCTTAAAAGACCTCGAGGACGTCGTCGGGCTGCTGAAGAAACTTGGCGTGAA
  GTTACAGGTCTCCATCAACCTGGGCTTGGTCTACAAGGTGCAGCAGCACACTGGCATCATCTTCCAGTTC
  CTGGCATTCAGCAAACGCAGGCAAAGGGTTGTGCCTGAGATCCTTGCCGCCGGTGGCAGATACGACCTGC
  TGATTCCCAAGTTCAGAGGCCCACAGACTGTGGGGCCAGTCCCCACTGCTGTCGGTGTCAGCATAGCCAT
  AGACAAGATATTTGCTGCTGTCCTCAACATGGAAGAGCCTGTTACAGTGAGCTCCTGTGACCTCCTGGTT
  GTCAGTGTTGGCCAGATGTCCATGTCCAGGGCCATCAACCTAACCCAGAAACTGTGGACAGCGGGCATCA
  CTGCAGAAATCATGTATGACTGGTCCCAGTCCCAGGAAGAGTTACAAGAGTACTGCAGACACCATGAAAT
  CACCTATGTGGCTCTGGTCTCCGATAAAGAAGGAAGCCATGTCAAGGTCAAGTCCTTTGAGAAGGAGAGG
  CAAACAGAAAAGCGTGTATTGGAATCGGATCTTGTGGATCACGTTATGCAGAAACTAAGAACCAAAGTTG
  GTGACGAAAGAAATTTCAGAGACGCTTCTGATAATCTTGCCGTACAAACTCTGAAGGGGTCGTTTTCTAA
  TGCTTCAGGTTTGTTTGAAATCCATGGAACTACAGTAGTCCCCAACGTGATTGTTCTAGCACCAGAAAAG
  CTGTCAGCCAGCACCAGGAGGCGACATGAGATTCAGGTGCAAACCCGACTTCAGACTACACTTGCCAACT
  TACATCAGAAAAGCAGTGAAATTGAAATTTTGGCTGTGGACCTACCCAAGGAAACAATCTTACAGTTTCT
  ATCATTAGAGTGGGATGCTGATGAACAGGCATTTAACACAACTGTGAAGCAGCTGCTGTCACGCCTGCCA
  AAGCAAAGATACCTCAAACTCGTCTGCGATGAAATTTATAACATCAAAGTTGAAAAGAAGGTGTCAGTGC
  TGTTCCTGTACAGCTACAGAGATGACTACTACAGAATCCTATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225162 representing NM_013719
  Red=Cloning site Green=Tags(s)
  
  MAGGRGASGRGRAEPQESYSQRQDHELQALEAIYGSDFQDLRPDARGRVREPPEINLVLYPQGLAGEEVY
  VQVELRVKCPPTYPDVVPEIDLKNAKGLSNESVNLLKSHLEELAKKQCGEVMIFELAHHVQSFLSEHNKP
  PPKSFHEEMLERQAQEKQQRLLEARRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEITSLTNQD
  YASKRDPAGHRAAAILHGGSPDFVGNGKARTYSSGRSRRERQYSVCSGEPSPGSCDILHFSVGSPDQLMV
  HKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLV
  KLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSHSGPVPAHQLRKYTAQLLAGLDYLHSNSV
  VHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVFEQARVRFSDSALPYKTGKKGDVWRLGLLLLSL
  SQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKLPLVEQSPEDSGGQDYI
  ETVIPSNQLPSAAFFSETQKQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFR
  RIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAP
  PPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIFDNEDENSKSQN
  QDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEK
  GMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPE
  VQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKSVISWL
  LNHDPAKRPTAMELLKSELLPPPQMEESELHEVLHHTLANIDGKAYRTMMSQIFCQHISPAIDYTYDSDI
  LKGNFLIRTAKIQQLVCETIVRVFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRVP
  FARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSSLPTAETIYTIYEIIQEFPAL
  QERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYVILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFI
  EQKGDLQDLTPTINSLIKQKTGVAQLVKYSLKDLEDVVGLLKKLGVKLQVSINLGLVYKVQQHTGIIFQF
  LAFSKRRQRVVPEILAAGGRYDLLIPKFRGPQTVGPVPTAVGVSIAIDKIFAAVLNMEEPVTVSSCDLLV
  VSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKER
  QTEKRVLESDLVDHVMQKLRTKVGDERNFRDASDNLAVQTLKGSFSNASGLFEIHGTTVVPNVIVLAPEK
  LSASTRRRHEIQVQTRLQTTLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLP
  KQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013719
• ORF Size: 4944 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013719.2, NM_013719.3, NP_038747.2
• RefSeq Size: 5220
• RefSeq ORF: 4947
• Locus ID: 27103
• MW: 187 kDa