Ch25h (NM_009890) Mouse Tagged ORF Clone

CAT#: MR225185

Ch25h (Myc-DDK-tagged) - Mouse cholesterol 25-hydroxylase (Ch25h)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ch25h
• Synonyms: AI462618; m25OH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225185 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGCTACAACGGTTCGGAGCTCCAAGACCTGGGCTGTTCCAGCCAGCTGCTCCTGCAGCCCCTCT
  GGGACACCATAAGGACAAGGGAGGCGTTCACGCGCTCACCCATCTTCCCAGTCACCTTTTCTATCATCAC
  TTACGTGGGCTTCTGCCTACCGTTCGTGGTGCTGGACGTCCTGTATCCCTGGGTCCCCATCCTGCGACGC
  TACAAGATCCACCCGGACTTCTCGCCTTCCGTAAAGCAGCTTCTGCCTTGCCTGGGGCTGACACTCTACC
  AGCACCTGGTGTTCGTGTTCCCGGTGACGCTGCTGCACTGGGTGCGCAGCCCGGCGCTCCTCCCCCAGGA
  GGCCCCTGAGCTCGTCCAGCTCCTAAGTCACGTCCTGATCTGCCTGCTGCTCTTCGACACCGAGATCTTC
  GCGTGGCACCTGCTGCACCATAAGGTGCCCTGGCTGTACCGCACCTTCCACAAGGTGCATCACCAGAACT
  CGTCCTCCTTCGCGCTGGCGACCCAATACATGAGCTTCTGGGAGCTGCTTTCGCTGACCTTCTTCGACGT
  GCTGAACGTCGCGGTGCTTCGGTGTCACCCACTCACCATCTTTACCTTTCACGTGATTAACATCTGGCTG
  TCGGTGGAGGACCACTCGGGCTATGACTTCCCGTGGTCCACTCACAGACTTGTGCCCTTTGGCTGGTACG
  GGGGCGTGGCTCACCACGACATGCATCACTCTCAGTTTAACTGCAATTTTGCTCCTTACTTCACACACTG
  GGACAAAATGCTGGGCACTCTGCGGTCCGCGCCCCTGCCAGAGAGCCTTTGCGCCTGCGGTGAGCGCTGC
  GTGAACTCCAGAGAGCGATGCGCTGTACACTTGATCCAGAAGAAGAAACAGACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225185 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGCYNGSELQDLGCSSQLLLQPLWDTIRTREAFTRSPIFPVTFSIITYVGFCLPFVVLDVLYPWVPILRR
  YKIHPDFSPSVKQLLPCLGLTLYQHLVFVFPVTLLHWVRSPALLPQEAPELVQLLSHVLICLLLFDTEIF
  AWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSFWELLSLTFFDVLNVAVLRCHPLTIFTFHVINIWL
  SVEDHSGYDFPWSTHRLVPFGWYGGVAHHDMHHSQFNCNFAPYFTHWDKMLGTLRSAPLPESLCACGERC
  VNSRERCAVHLIQKKKQT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009890
• ORF Size: 897 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009890.1, NP_034020.1
• RefSeq Size: 1366
• RefSeq ORF: 897
• Locus ID: 12642
• MW: 34.7 kDa