Pak1 (NM_011035) Mouse Tagged ORF Clone

CAT#: MR225277

Pak1 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 1 (Pak1). Note: ORF is codon optimized

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pak1
• Synonyms: AW045634; PAK-1; Paka
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225277 representing NM_011035, codon optimized.
  Due to the complexity of NM_011035, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAATAACGGCGTTGATATACAGGACAAACCACCCGCCCCTCCCATGAGGAATACATCCACCATGA
  TCGGCGCCGGCAGCAAGGATACGGGTACTCTCAACCATGGCTCTAAGCCCCTGCCGCCAAATCCCGAAGA
  AAAAAAAAAGAAGGATAGATTCTATAGAAGCATTCTGCCCGGGGATAAGACAAACAAGAAGAAAGAGAAG
  GAACGCCCGGAAATCAGTCTCCCTTCTGATTTTGAGCACACAATTCATGTTGGCTTCGATGCAGTAACCG
  GAGAGTTTACAGGGATGCCGGAGCAGTGGGCCAGGCTGCTGCAGACATCCAATATCACAAAGAGCGAGCA
  GAAAAAGAACCCACAGGCAGTGCTCGACGTGCTGGAGTTCTACAACAGTAAAAAGACCTCAAACAGTCAA
  AAGTACATGAGTTTTACCGACAAGAGTGCAGAGGATTACAACTCCTCCAACACACTGAACGTGAAGACAG
  TTTCAGAAACCCCAGCCGTTCCCCCAGTATCTGAGGACGAAGATGATGACGATGATGCCACACCACCACC
  CGTTATCGCCCCTAGGCCCGAGCACACTAAATCAGTTTATACCAGAAGTGTGATTGAACCGCTCCCGGTC
  ACCCCTACCCGAGATGTGGCTACCTCACCCATCAGTCCTACCGAAAACAACACTACCCCACCGGATGCCC
  TGACTCGAAACACTGAGAAACAGAAGAAGAAGCCAAAAATGTCCGATGAAGAGATTCTGGAGAAGCTGAG
  GTCTATTGTGAGTGTGGGGGACCCCAAGAAGAAGTACACCCGGTTCGAAAAGATCGGCCAGGGAGCAAGC
  GGCACTGTGTACACCGCCATGGACGTCGCCACTGGACAAGAAGTAGCAATCAAGCAAATGAATCTGCAGC
  AGCAGCCAAAGAAGGAACTGATCATTAATGAGATTCTTGTGATGCGGGAAAACAAAAACCCAAATATTGT
  GAACTACCTCGATAGTTATCTGGTAGGCGATGAGCTCTGGGTGGTGATGGAATACTTGGCTGGTGGATCA
  CTCACAGACGTCGTGACCGAAACCTGTATGGACGAGGGTCAGATTGCTGCGGTCTGTAGAGAGTGCTTGC
  AGGCTCTGGAGTTTCTGCACAGCAACCAAGTGATCCATCGCGACATCAAAAGCGACAACATCTTGCTCGG
  TATGGATGGGAGTGTCAAGCTCACAGACTTCGGATTTTGTGCGCAGATCACGCCCGAGCAGAGCAAAAGA
  AGCACAATGGTGGGGACCCCCTACTGGATGGCCCCTGAAGTTGTTACACGCAAGGCTTACGGACCAAAGG
  TAGACATTTGGTCACTGGGTATTATGGCTATAGAGATGATTGAGGGAGAACCACCATACCTGAACGAGAA
  CCCACTCCGGGCCCTGTACCTGATCGCTACTAATGGGACACCAGAACTGCAGAACCCCGAGAAACTCTCT
  GCGATCTTTCGAGATTTCCTGAATCGCTGCCTTGAGATGGATGTGGAAAAAAGGGGTAGCGCTAAAGAGC
  TGCTTCAGCATCAGTTCCTTAAAATCGCCAAACCACTGTCAAGCCTTACTCCATTGATCGCTGCAGCTAA
  GGAGGCTACCAAAAATAACCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225277 representing NM_011035
  Red=Cloning site Green=Tags(s)
  
  MSNNGVDIQDKPPAPPMRNTSTMIGAGSKDTGTLNHGSKPLPPNPEEKKKKDRFYRSILPGDKTNKKKEK
  ERPEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLEFYNSKKTSNSQ
  KYMSFTDKSAEDYNSSNTLNVKTVSETPAVPPVSEDEDDDDDATPPPVIAPRPEHTKSVYTRSVIEPLPV
  TPTRDVATSPISPTENNTTPPDALTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGAS
  GTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGS
  LTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKR
  STMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLS
  AIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011035
• ORF Size: 1632 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011035.2, NP_035165.2
• RefSeq Size: 3081
• RefSeq ORF: 1635
• Locus ID: 18479
• MW: 60.6 kDa