Chrd (NM_009893) Mouse Tagged ORF Clone

CAT#: MR225319

Chrd (Myc-DDK-tagged) - Mouse chordin (Chrd)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chrd
• Synonyms: Chd
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225319 representing NM_009893
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAGCCTCCCGGCCCCGCCGGCCCCGCGGCTGCTCCTCGGGCTGCTGCTGCTCGGCTCGCGGCCGG
  CCAGTGGCACTGGCCCTGAGCCCCCAGCACTGCCCATCCGTTCCGAGAAGGAGCCGCTGCCTGTCCGGGG
  AGCGGCAGGCTGCTCCTTCGGCGGGAAGGTCTATGCCTTGGACGAGACGTGGCACCCGGACCTGGGGGAG
  CCTTTTGGGGTGATGCGCTGCGTGCTGTGCGCCTGTGAAGCGCCTCAGTGGGCTCGCCGTGGGAGGGGTC
  CTGGCAGGGTCAGCTGCAAGAACATCAAACCTCAGTGCCCCACCCTGGCCTGCAGGCAGCCGCGCCAGCT
  GCCAGGACACTGCTGCCAGACCTGCCCGCAGGAGCGCAGCAATCTAGATCCACAGCCCGCTGGCCTGGTC
  TTCGAGTATCCAAGGGATCCAGAGCATCGCAGTTACAGTGATCGAGGGGAACCCGGCGTTGGGGAGCGGA
  CCCGTGCTGATGGCCACACAGACTTTGTGGCGCTGCTGACAGGACCGAGGTCGCAGGCGGTAGCTCGTGC
  TCGAGTCTCTTTGCTGCGCTCAAGTTTACGCTTCTCTGTCTCCTACCAGCGGCTGGACCGTCCCAGCAGG
  GTTCGTTTCACAGATCCCACAGGCAACATCCTGTTTGAACACCCTGCAACCCCCACCCAGGATGGCCTGG
  TCTGTGGGGTGTGGCGGGCAGTGCCTCGGCTGTCTGTGAGGCTCCTGAGGGCAGAGCAGCTTCGGGTAGC
  CCTTGTGACATCCACTCACCCTTCAGGAGAAGTCTGGGGGCCTCTCATTTGGCAGGGTGCTCTCGCTGCA
  GAGACCTTCAGTGCCATCCTGACCCTGGAAGACCCACTGCAGCGGGGTGTGGGGGGCATAGCCCTGCTCA
  CCCTCAGTGACACAGAAGATTCCTTGCATTTTTTGCTGCTCTTCCGGGGTCTGCTGGGAGGACTAGCTCA
  GGCTCCCTTGAAGCTTCAGATTCTCCACCAGGGACAGCTACTTCGGGAGCTCCAGGCCAACACCTCCGCT
  CAGGAGCCAGGTTTTGCTGAGGTGCTGCCCAGCCTTACAGACCAAGAGATGGACTGGTTGGAGCTGGGGG
  AGCTGCAGATGGTCCTAGAGAAGGCGGGTGGGCCAGAGCTACGCATCAGTGGATACATCACCACCAGGCA
  GAGCTGTGATGTCCTTCAAAGTGTCCTTTGTGGGGCTGATGCCCTGATCCCAGTCCAGACGGGTGCTGCT
  GGGTCAGCCAGCTTCATATTGCTAGGAAATGGCTCCCTTATCTATCAGGTGCAAGTGGTAGGTACAGGTA
  GCGAGGTGGTGGCCATGACACTGGAGACCAAGCCTCAGCGGAAGAACCAGCGCACTGTCCTGTGCCACAT
  GGCTGGACTCCAGCCGGGAGGACACATGGCTGTGGGTATGTGCTCTGGGCTGGGTGCCCGAGGGGCTCAT
  ATGCTGCTACAGAACGAGCTGTTCCTGAATGTTGGTACCAAGGACTTCCCAGATGGAGAGCTTCGGGGGC
  ATGTGACTGCCCTATGCTACAGTGGGCACAGTGCCCGCTATGACAGATTGCCTGTGCCTCTGGCAGGGGC
  ACTAGTGCTGCCCCCTGTGCGGAGTCAGGCAGCAGGGCACGCCTGGCTCTCCTTGGATACACATTGTCAC
  TTACACTATGAAGTTCTATTGGCTGGGCTTGGTGGCTCAGAGCAAGGAACTGTCACTGCCCACCTCCTTG
  GGCCTCCTGGGATGCCAGGGCCCCAGCGGCTGCTGAAGGGATTCTATGGCTCAGAGGCTCAGGGCGTGGT
  AAAAGATCTGGAGCCTGTGCTGCTGCGGCACCTGGCACAGGGCACTGCCTCCCTGCTCATCACCACCAAG
  AGTAGCCCCAGAGGAGAACTACGTGGGCAGGTGCACATTGCCAGTCAGTGTGAGGCGGGAGGCCTGCGTC
  TGGCCTCAGAAGGAGTGCAGATGCCCTTGGCTCCAAATGGAGAGGCGGCTACGTCACCCATGCTGCCTGC
  TGGCCCTGGCCCTGAAGCCCCAGTACCAGCCAAACATGGCAGCCCTGGGAGACCCCGAGATCCTAACACA
  TGTTTCTTCGAGGGGCAGCAGCGGCCCCACGGGGCTCGCTGGGCACCCAACTATGACCCACTCTGCTCCC
  TCTGCATCTGCCAGAGACGAACAGTGATCTGTGACCCTGTAGTATGCCCACCACCAAGCTGTCCCCACCC
  GGTGCAGGCACTGGACCAGTGCTGTCCCGTGTGTCCAGAGAAACAACGCAGTAGAGACCTCCCCAGCCTA
  CCAAATCTGGAGCCAGGAGAGGGCTGCTATTTTGATGGTGACCGGAGCTGGAGGGCAGCGGGTACCCGAT
  GGCACCCTGTTGTGCCCCCCTTTGGCCTAATTAAGTGTGCTGTCTGCACCTGCAAGGGGGCCACGGGAGA
  GGTGCACTGTGAGAAGGTGCAGTGTCCTCGCCTGGCCTGTGCTCAGCCTGTCCGTGCCAACCCCACCGAC
  TGCTGCAAACAGTGTCCAGTAGGGTCAGGGACTAATGCCAAGCTGGGAGACCCCATGCAGGCTGATGGGC
  CTCGGGGGTGTCGCTTTGCTGGGCAGTGGTTCCCAGAGAATCAGAGCTGGCACCCATCAGTGCCCCCCTT
  TGGGGAGATGAGCTGTATTACCTGCAGATGTGGGGCAGGGGTACCCCACTGTGAGCGGGACGATTGTTCA
  CCGCCGCTATCCTGTGGCTCAGGGAAGGAGAGTCGATGCTGTTCCCACTGCACAGCCCAAAGGTCCTCTG
  AGACTAGAACCCTTCCAGAGCTGGAGAAAGAAGCGGAGCACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225319 representing NM_009893
  Red=Cloning site Green=Tags(s)
  
  MPSLPAPPAPRLLLGLLLLGSRPASGTGPEPPALPIRSEKEPLPVRGAAGCSFGGKVYALDETWHPDLGE
  PFGVMRCVLCACEAPQWARRGRGPGRVSCKNIKPQCPTLACRQPRQLPGHCCQTCPQERSNLDPQPAGLV
  FEYPRDPEHRSYSDRGEPGVGERTRADGHTDFVALLTGPRSQAVARARVSLLRSSLRFSVSYQRLDRPSR
  VRFTDPTGNILFEHPATPTQDGLVCGVWRAVPRLSVRLLRAEQLRVALVTSTHPSGEVWGPLIWQGALAA
  ETFSAILTLEDPLQRGVGGIALLTLSDTEDSLHFLLLFRGLLGGLAQAPLKLQILHQGQLLRELQANTSA
  QEPGFAEVLPSLTDQEMDWLELGELQMVLEKAGGPELRISGYITTRQSCDVLQSVLCGADALIPVQTGAA
  GSASFILLGNGSLIYQVQVVGTGSEVVAMTLETKPQRKNQRTVLCHMAGLQPGGHMAVGMCSGLGARGAH
  MLLQNELFLNVGTKDFPDGELRGHVTALCYSGHSARYDRLPVPLAGALVLPPVRSQAAGHAWLSLDTHCH
  LHYEVLLAGLGGSEQGTVTAHLLGPPGMPGPQRLLKGFYGSEAQGVVKDLEPVLLRHLAQGTASLLITTK
  SSPRGELRGQVHIASQCEAGGLRLASEGVQMPLAPNGEAATSPMLPAGPGPEAPVPAKHGSPGRPRDPNT
  CFFEGQQRPHGARWAPNYDPLCSLCICQRRTVICDPVVCPPPSCPHPVQALDQCCPVCPEKQRSRDLPSL
  PNLEPGEGCYFDGDRSWRAAGTRWHPVVPPFGLIKCAVCTCKGATGEVHCEKVQCPRLACAQPVRANPTD
  CCKQCPVGSGTNAKLGDPMQADGPRGCRFAGQWFPENQSWHPSVPPFGEMSCITCRCGAGVPHCERDDCS
  PPLSCGSGKESRCCSHCTAQRSSETRTLPELEKEAEHS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009893
• ORF Size: 2844 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009893.1, NM_009893.2, NP_034023.1
• RefSeq Size: 3292
• RefSeq ORF: 2847
• Locus ID: 12667
• MW: 102 kDa
• Gene Summary: Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes. [UniProtKB/Swiss-Prot Function]