Limk1 (NM_010717) Mouse Tagged ORF Clone
CAT#: MR225384
- TrueORF®
Limk1 (Myc-DDK-tagged) - Mouse LIM-domain containing, protein kinase (Limk1)
"NM_010717" in other vectors (4)
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Product Images
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Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Limk1 |
Synonyms | KIZ-1; Limk; LIMK-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225384 representing NM_010717
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGTTGACGCTACTTTGTTGCACCTGGAGGGAAGAACGTATGGGAGAGGAAGGAAGCGAGTTGCCTG TGTGTGCGAGCTGTGGCCAGAGGATCTATGACGGCCAGTACCTCCAGGCCCTGAATGCTGACTGGCATGC AGACTGCTTCAGGTGCTGCGAGTGTAGCGTCTCCCTTTCACACCAGTACTACGAGAAGGATGGACAGCTC TTCTGCAAGAAGGACTACTGGGCCCGCTATGGCGAGTCTTGCCACGGGTGCTCCGAGCACATCACCAAAG GGCTGGTCATGGTGGCTGGGGAGCTGAAGTACCACCCTGAGTGTTTCATCTGCCTCGCCTGTGGAAACTT CATTGGCGATGGGGACACCTACACACTGGTGGAGCACTCCAAGCTGTACTGTGGCCAGTGCTACTACCAG ACCGTGGTAACTCCAGTCATCGAACAGATCCTACCTGACTCACCCGGCTCCCACCTGCCCCACACAGTCA CCCTCGTGTCTATCCCAGCCTCTGCCCATGGCAAACGAGGCCTGTCTGTCTCCATCGACCCCCCTCACGG CCCACCAGGATGTGGCACGGAGCATTCGCACACTGTCCGAGTCCAAGGAGTGGACCCAGGCTGCATGAGC CCAGATGTGAAGAATTCCATCCACGTTGGAGACCGGATCTTGGAAATCAATGGCACGCCCATCCGGAATG TGCCGCTGGACGAGATCGACTTGCTGATCCAGGAGACCAGCCGCCTGCTCCAGCTGACCCTCGAGCACGA CCCCCATGACTCACTGGGCCATGGGCCCGTGTCGGATCCCAGCCCCCTGAGCTCCCCGGTGCACACTCCC AGCGGACAGGCAGCCAGCTCTGCCCGGCAGAAACCTGTCTTGAGGAGCTGCAGTATTGACACGTCCCCGG GTACCAGCTCACTAGCCTCTCCAGCCTCCCAGCGCAAGGACCTGGGTCGCTCCGAATCCCTCCGAGTGGT CTGCCGACCACACCGAATCTTCCGGCCATCTGATCTCATCCATGGGGAAGTGCTGGGCAAGGGCTGTTTT GGCCAGGCCATCAAGGTGACACACCGAGAAACAGGCGAGGTGATGGTGATGAAGGAACTTATCCGGTTTG ATGAGGAGACCCAGAGGACATTCCTCAAGGAGGTAAAGGTCATGCGCTGCCTGGAGCACCCTAACGTGCT CAAGTTCATCGGAGTGCTCTACAAGGACAAGCGGCTAAACTTCATCACAGAGTACATCAAGGGCGGCACC CTGCGGGGCATCATCAAGAACATGGACAGCCAGTACCCGTGGAGTCAGAGGGTCAGCTTTGCCAAGGACA TTGCATCAGGGATGGCCTACCTCCATTCGATGAACATCATCCACCGAGACCTCAACTCCCACAACTGTCT GGTCCGTGAGAATAGGAACGTGGTGGTGGCCGACTTTGGGCTGGCTCGACTCATGATCGATGAAAAGAAT CAGTCTGAAGACTTACGCAGCCTTAAGAAGCCGG[AT]CCGCAAGAAGCGGTACACAGTGGTGGGCAACC CCTACTGGATGGCGCCAGAGATGATCAATGGTCGTAGTTATGATGAAAAGGTGGACGTGTTTTCCTTTGG AATTGTCCTCTGTGAGATTATCGGGCGTGTGAATGCAGACCCTGACTATCTGCCCCGCACTATGGACTTT GGCCTCAATGTAAGGGGTTTCCTGGACCGCTACTGTCCACCAAACTGTCCCCCAAGTTTCTTCCCCATCA CTGTGCGCTGCTGTGACCTGGATCCTGAAAAGAGACCCTCTTTTGTAAAGCTGGAACAATGGCTAGAAAC ACTTCGCATGCACTTGTCTGGTCACCTGCCACTGGGCCCACAACTGGAACAACTGGAGCGGGGCTTCTGG GAGACCTACCGGCGGGGCGAGAGCTCACTGCCTGCCCACCCCGAGGTCCCTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225384 representing NM_010717
Red=Cloning site Green=Tags(s) MRLTLLCCTWREERMGEEGSELPVCASCGQRIYDGQYLQALNADWHADCFRCCECSVSLSHQYYEKDGQL FCKKDYWARYGESCHGCSEHITKGLVMVAGELKYHPECFICLACGNFIGDGDTYTLVEHSKLYCGQCYYQ TVVTPVIEQILPDSPGSHLPHTVTLVSIPASAHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMS PDVKNSIHVGDRILEINGTPIRNVPLDEIDLLIQETSRLLQLTLEHDPHDSLGHGPVSDPSPLSSPVHTP SGQAASSARQKPVLRSCSIDTSPGTSSLASPASQRKDLGRSESLRVVCRPHRIFRPSDLIHGEVLGKGCF GQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGT LRGIIKNMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENRNVVVADFGLARLMIDEKN QSEDLRSLKKPXXRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDF GLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEQWLETLRMHLSGHLPLGPQLEQLERGFW ETYRRGESSLPAHPEVPD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010717 |
ORF Size | 1944 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_010717.1, NM_010717.2, NM_010717.3, NP_034847.1 |
RefSeq Size | 3323 |
RefSeq ORF | 1944 |
Locus ID | 16885 |
MW | 73.2 kDa |
Gene Summary | This gene encodes a member of the LIM kinase family of proteins. This protein is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein also stimulates axon growth and may play a role in brain development. Homozygous knockout mice for this gene exhibit reduced bone mass, abnormal neuronal morphology and altered synaptic function. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220064 | Limk1 (untagged) - Mouse LIM-domain containing, protein kinase (Limk1), (10ug) |
USD 880.00 |
|
MG225384 | Limk1 (GFP-tagged) - Mouse LIM-domain containing protein kinase (Limk1), (10ug) |
USD 660.00 |
|
MR225384L3 | Lenti ORF clone of Limk1 (Myc-DDK-tagged) - Mouse LIM-domain containing, protein kinase (Limk1) |
USD 800.00 |
|
MR225384L4 | Lenti ORF clone of Limk1 (mGFP-tagged) - Mouse LIM-domain containing, protein kinase (Limk1) |
USD 800.00 |
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