Bmp4 (NM_007554) Mouse Tagged ORF Clone

CAT#: MR225467

Bmp4 (Myc-DDK-tagged) - Mouse bone morphogenetic protein 4 (Bmp4)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bmp4
• Synonyms: Bmp-4; Bmp2b; Bmp2b-1; Bmp2b1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225467 representing NM_007554
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCCTGGTAACCGAATGCTGATGGTCGTTTTATTATGCCAAGTCCTGCTAGGAGGCGCGAGCCATG
  CTAGTTTGATACCTGAGACCGGGAAGAAAAAAGTCGCCGAGATTCAGGGCCACGCGGGAGGACGCCGCTC
  AGGGCAGAGCCATGAGCTCCTGCGGGACTTCGAGGCGACACTTCTACAGATGTTTGGGCTGCGCCGCCGT
  CCGCAGCCTAGCAAGAGCGCCGTCATTCCGGATTACATGAGGGATCTTTACCGGCTCCAGTCTGGGGAGG
  AGGAGGAGGAAGAGCAGAGCCAGGGAACCGGGCTTGAGTACCCGGAGCGTCCCGCCAGCCGAGCCAACAC
  TGTGAGGAGTTTCCATCACGAAGAACATCTGGAGAACATCCCAGGGACCAGTGAGAGCTCTGCTTTTCGT
  TTCCTCTTCAACCTCAGCAGCATCCCAGAGAATGAGGTGATCTCCTCGGCAGAGCTCCGGCTCTTTCGGG
  AGCAGGTGGACCAGGGCCCTGACTGGGAACAGGGCTTCCACCGTATAAACATTTATGAGGTTATGAAGCC
  CCCAGCAGAAATGGTTCCTGGACACCTCATCACACGACTACTGGACACCAGACTAGTCCATCACAATGTG
  ACACGGTGGGAAACTTTCGATGTGAGCCCTGCAGTCCTTCGCTGGACCCGGGAAAAGCAACCCAATTATG
  GGCTGGCCATTGAGGTGACTCACCTCCACCAGACACGGACCCACCAGGGCCAGCACGTCAGAATCAGCCG
  ATCGTTACCTCAAGGGAGTGGAGATTGGGCCCAACTCCGGCCCCTCCTGGTCACTTTTGGCCATGATGGC
  CGGGGCCATACCTTGACCCGCAGAAGGGCCAAACGTAGTCCCAAGCATCACCCACAGCGGTCCAGGAAGA
  AGAATAAGAACTGCCGTCGCCATTCACTATACGTGGACTTCAGTGACGTGGGCTGGAATGATTGGATTGT
  GGCCCCACCCGGCTACCAGGCCTTCTACTGCCACGGGGACTGTCCCTTTCCACTGGCTGATCACCTCAAC
  TCAACCAACCATGCCATTGTGCAGACCCTAGTCAACTCTGTTAATTCTAGTATCCCTAAGGCCTGTTGTG
  TCCCCACTGAACTGAGTGCCATTTCCATGTTGTACCTGGATGAGTATGACAAGGTGGTGTTGAAAAATTA
  TCAGGAGATGGTGGTAGAGGGGTGTGGATGCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225467 representing NM_007554
  Red=Cloning site Green=Tags(s)
  
  MIPGNRMLMVVLLCQVLLGGASHASLIPETGKKKVAEIQGHAGGRRSGQSHELLRDFEATLLQMFGLRRR
  PQPSKSAVIPDYMRDLYRLQSGEEEEEEQSQGTGLEYPERPASRANTVRSFHHEEHLENIPGTSESSAFR
  FLFNLSSIPENEVISSAELRLFREQVDQGPDWEQGFHRINIYEVMKPPAEMVPGHLITRLLDTRLVHHNV
  TRWETFDVSPAVLRWTREKQPNYGLAIEVTHLHQTRTHQGQHVRISRSLPQGSGDWAQLRPLLVTFGHDG
  RGHTLTRRRAKRSPKHHPQRSRKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLN
  STNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007554
• ORF Size: 1224 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007554.2, NM_007554.3, NP_031580.2
• RefSeq Size: 1994
• RefSeq ORF: 1227
• Locus ID: 12159
• MW: 46.9 kDa
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Homozygous knockout mice die in utero, while a conditional knockout mouse exhibits defects in heart development. Transgenic mice overexpressing this gene in a neuron-specific manner exhibit a phenotype resembling the rare hereditary connective tissue disease, fibrodysplasia ossificans progressiva. [provided by RefSeq, Jul 2016]