Pde4d (NM_011056) Mouse Tagged ORF Clone

CAT#: MR225474

Pde4d (Myc-DDK-tagged) - Mouse phosphodiesterase 4D, cAMP specific (Pde4d)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde4d
• Synonyms: 9630011N22Rik; Dpde3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225474 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGGGACACTTGTGATGTACTCTCTCGGAGCAAAAGTGCCTCTGAGGAAACACTGCACTCCTGTA
  ATGAAGAAGAGGACCCTTTTCGTGGAATGGAGCCCTATCTCGTACGGCGACTTTCTTCCCGCAGCATTCA
  GCTTCCACCTCTGGCCTTCAGACAGTTGGAACAAGCAGACTTGCGAAGCGAATCAGAGAACATCCCTCGG
  CCAACTAGCCTCCCCTTGAAAATCCTGCCTCTGATCGCCGTCACATCTGCAGACTCAAGTGGTTTCGATG
  TGGACAATGGCACATCAGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCAGGGTCTGGGCTGATTCT
  CCAAGCAAACTTTGTCCACAGTCAACGCCGGGAGTCCTTCCTGTACCGATCTGACAGCGACTATGACCTC
  TCTCCAAAGTCTATGTCCAGGAACTCCTCGATTGCCAGTGATATACATGGAGATGACTTGATTGTGACCC
  CGTTTGCTCAGGTCTTGGCTAGCCTGCGAACCGTACGGAACAACTTTGCTGCGTTAACGAATTTGCAAGA
  CCGAGCACCCAGCAAAAGATCACCCATGTGCAACCAACCATCCATCAACAAAGCCACCATCACAGAGGAG
  GCCTACCAGAAACTGGCCAGCGAGACCCTGGAGGAGCTGGACTGGTGTCTGGACCAGCTAGAGACCCTGC
  AGACCAGGCACTCCGTCAGTGAGATGGCCTCCAACAAGTTCAAAAGGATGCTGAACCGGGAGCTCACGCA
  TCTCTCTGAGATGAGTCGGTCTGGCAACCAGGTGTCGGAGTACATCTCAAACACATTTCTCGATAAGCAA
  CATGAAGTGGAAATCCCCTCTCCAACTCAGAAGGAAAAGGAGAAGAAGAAAAGGCCGATGTCGCAGATCA
  GTGGGGTCAAGAAGTTGATGCACAGCTCCAGCCTAACTAATTCATGTATCCCCAGGTTTGGGGTTAAAAC
  GGAGCAGGAAGATGTCCTGGCCAAGGAACTAGAAGACGTGAACAAGTGGGGCCTCCACGTTTTCCGAATA
  GCAGAGCTGTCTGGTAACCGGCCTCTGACTGTTATCATGCACACCATTTTTCAGGAACGAGATTTGTTAA
  AAACGTTTAAAATCCCAGTAGACACTTTGATTACGTATCTTATGACTCTGGAAGACCATTACCATGCTGA
  CGTGGCCTATCACAACAACATCCATGCTGCAGACGTCGTCCAGTCCACTCACGTGCTGCTCTCTACACCC
  GCTTTGGAGGCTGTGTTCACAGACTTGGAGATTCTCGCGGCCATTTTTGCCAGTGCAATACATGATGTGG
  ACCATCCTGGGGTGTCAAATCAATTTCTGATCAATACAAACTCGGAACTCGCTCTGATGTACAACGACTC
  CTCGGTCCTAGAGAACCATCACCTGGCGGTGGGCTTTAAGTTGCTCCAGGAAGAAAACTGTGACATTTTC
  CAGAATCTGACCAAAAAGCAAAGACAATCTTTAAGGAAAATGGTCATTGACATTGTCCTGGCGACAGACA
  TGTCGAAGCACATGAACCTGCTGGCTGATCTGAAAACCATGGTTGAAACCAAGAAGGTGACAAGCTCCGG
  CGTCCTCCTCCTGGATAACTACTCCGACAGGATCCAGGTCCTTCAGAATATGGTGCACTGTGCAGACCTG
  AGCAACCCCACAAAGCCACTCCAGCTGTACCGCCAGTGGACGGACCGGATAATGGAGGAGTTCTTCCGCC
  AGGGGGACCGAGAGCGGGAGCGTGGCATGGAGATAAGTCCCATGTGTGACAAGCACAACGCCTCTGTGGA
  AAAATCACAGGTGGGCTTCATAGACTATATCGTTCATCCACTCTGGGAGACGTGGGCAGACCTCGTACAT
  CCCGATGCCCAGGACATCTTGGACACTTTGGAGGACAATCGTGAGTGGTACCAGAGCACAATCCCCCAGA
  GCCCCTCCCCTGCACCTGATGACCAAGAGGAGGGCCGGCAGGGACAGACTGAAAAATTCCAGTTTGAACT
  AACCTTGGAGGAAGACTGTGAGTCAGACACTGAAAAGGACAGTGGAAGTCAGGTGGAGGAAGACACTAGC
  TGCAGTGACTCTAAGACTCTGTGCACCCAAGACTCAGAGTCCACTGAAATTCCCCTTGACGAGCAGGTTG
  AGGAGGAAGCTGTAGCAGAAGAGGAAAGCCAGCCTGAAACTTGCGTCCCAGATGACTGCTGTCCTGATAC
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225474 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MERDTCDVLSRSKSASEETLHSCNEEEDPFRGMEPYLVRRLSSRSIQLPPLAFRQLEQADLRSESENIPR
  PTSLPLKILPLIAVTSADSSGFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDL
  SPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEE
  AYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQ
  HEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRI
  AELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTP
  ALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIF
  QNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADL
  SNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVH
  PDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEEGRQGQTEKFQFELTLEEDCESDTEKDSGSQVEEDTS
  CSDSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPETCVPDDCCPDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011056
• ORF Size: 2244 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011056.1, NM_011056.2, NM_011056.3, NP_035186.1
• RefSeq Size: 7049
• RefSeq ORF: 2244
• Locus ID: 238871
• MW: 84.6 kDa