Crbn (NM_175357) Mouse Tagged ORF Clone

CAT#: MR225531

Crbn (Myc-DDK-tagged) - Mouse cereblon (Crbn), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Crbn
• Synonyms: 2610203G15Rik; 2900045O07Rik; AF229032; AW108261; piL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225531 representing NM_175357
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCGAGGGAGATCAGCAGGACGCTGCGCACAACATGGGAAACCACCTGCCGCTTCTGCCTGCAG
  ACAGTGAAGATGAAGATGATGAAATTGAAATGGAAGTTGAAGACCAAGATAGTAAAGAAGCCAGAAAACC
  GAATATCATAAACTTTGACACCAGTCTGCCAACCTCACATACATACCTGGGAGCTGATATGGAGGAGTTC
  CACGGGAGAACTTTGCATGACGACGACAGCTGCCAGGTGATCCCAGTCCTTCCTGAGGTGCTGATGATCC
  TGATTCCTGGGCAGACACTCCCACTGCAGCTCTCTCACCCACAGGAAGTCAGCATGGTGCGGAACTTAAT
  CCAGAAAGACAGGACCTTTGCAGTCCTTGCATACAGTAATGTGCAAGAAAGGGAAGCACAGTTTGGGACA
  ACAGCAGAGATCTATGCCTATCGAGAAGAGCAGGAGTTTGGAATTGAAGTAGTGAAAGTGAAAGCAATTG
  GAAGGCAGCGGTTCAAGGTCCTCGAACTTCGAACACAGTCAGATGGAATCCAGCAAGCTAAAGTGCAGAT
  TTTGCCAGAGTGTGTGTTGCCGTCAACCATGTCTGCAGTGCAGTTAGAATCACTCAATAAGTGCCAGGTA
  TTTCCTTCAAAACCCATCTCCTGGGAAGACCAGTATTCATGTAAATGGTGGCAGAAATACCAGAAGAGAA
  AGTTTCACTGTGCAAATCTAACATCATGGCCTCGCTGGCTGTATTCATTATATGATGCTGAAACATTAAT
  GGATAGAATTAAGAAACAGCTACGTGAATGGGATGAAAATCTCAAAGATGATTCTCTTCCTGAAAATCCA
  ATAGACTTTTCTTACAGAGTAGCTGCTTGTCTTCCTATTGATGATGTATTGAGAATTCAGCTCCTTAAAA
  TCGGCAGTGCTATTCAACGGCTTCGCTGTGAATTGGACATCATGAACAAATGTACTTCCCTTTGCTGTAA
  ACAATGTCAAGAAACAGAAATAACGACAAAGAATGAAATATTTAGTTTATCCTTATGTGGTCCAATGGCA
  GCATATGTGAATCCTCATGGATATGTACATGAGACACTGACTGTGTATAAAGCGTCCAACCTGAATCTGA
  TAGGCCGGCCTTCTACAGTGCACAGCTGGTTTCCCGGGTATGCATGGACCATTGCCCAGTGCAAGATCTG
  TGCAAGCCATATTGGATGGAAATTTACAGCCACAAAAAAAGACATGTCACCTCAAAAATTTTGGGGCTTA
  ACTCGCTCTGCTCTGTTACCCACAATTCCAGAGACTGAAGATGAAATAAGTCCAGACAAAGTAATACTTT
  GTTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225531 representing NM_175357
  Red=Cloning site Green=Tags(s)
  
  MAGEGDQQDAAHNMGNHLPLLPADSEDEDDEIEMEVEDQDSKEARKPNIINFDTSLPTSHTYLGADMEEF
  HGRTLHDDDSCQVIPVLPEVLMILIPGQTLPLQLSHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGT
  TAEIYAYREEQEFGIEVVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQV
  FPSKPISWEDQYSCKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPENP
  IDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMA
  AYVNPHGYVHETLTVYKASNLNLIGRPSTVHSWFPGYAWTIAQCKICASHIGWKFTATKKDMSPQKFWGL
  TRSALLPTIPETEDEISPDKVILCL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175357
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_175357.1, NM_175357.2, NM_175357.3, NP_780566.1
• RefSeq Size: 4054
• RefSeq ORF: 1338
• Locus ID: 58799
• MW: 51.3 kDa
• Gene Summary: This gene encodes a protein with a Lon protease domain, a "regulators of G protein-signaling" (RGS)-like domain and a leucine zipper. It has been proposed to regulate the assembly and surface expression of large-conductance calcium-activated potassium channels in brain and to bind thalidomide. In humans mutation in this gene causes autosomal recessive nonsyndromic cognitive disability. In mouse deficiency of this gene serves as a model to study the molecular mechanisms governing learning and memory as they relate to intellectual disability. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]