Dnmt3b (NM_001122997) Mouse Tagged ORF Clone

CAT#: MR225606

Dnmt3b (Myc-DDK-tagged) - Mouse DNA methyltransferase 3B (Dnmt3b), transcript variant 5

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnmt3b
• Synonyms: MmuIIIB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225606 representing NM_001122997
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGAGACAGCAGACATCTGAATGAAGAAGAGGGTGCCAGCGGGTATGAGGAGTGCATTATCGTTA
  ATGGGAACTTCAGTGACCAGTCCTCAGACACGAAGGATGCTCCCTCACCCCCAGTCTTGGAGGCAATCTG
  CACAGAGCCAGTCTGCACACCAGAGACCAGAGGCCGCAGGTCAAGCTCCCGGCTGTCTAAGAGGGAGGTC
  TCCAGCCTTCTGAATTACACGCAGGACATGACAGGAGATGGAGACAGAGATGATGAAGTAGATGATGGGA
  ATGGCTCTGATATTCTAATGCCAAAGCTCACCCGTGAGACCAAGGACACCAGGACGCGCTCTGAAAGCCC
  GGCTGTCCGAACCCGACATAGCAATGGGACCTCCAGCTTGGAGAGGCAAAGAGCCTCCCCCAGAATCACC
  CGAGGTCGGCAGGGCCGCCACCATGTGCAGGAGTACCCTGTGGAGTTTCCGGCTACCAGGTCTCGGAGAC
  GTCGAGCATCGTCTTCAGCAAGCACGCCATGGTCATCCCCTGCCAGCGTCGACTTCATGGAAGAAGTGAC
  ACCTAAGAGCGTCAGTACCCCATCAGTTGACTTGAGCCAGGATGGAGATCAGGAGGGTATGGATACCACA
  CAGGTGGATGCAGAGAGCAGAGATGGAGACAGCACAGAGTATCAGGATGATAAAGAGTTTGGAATAGGTG
  ACCTCGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCTGCCATGGTGGTGTCCTGGAAAGCCACCTC
  CAAGCGACAGGCCATGCCCGGAATGCGCTGGGTACAGTGGTTTGGTGATGGCAAGTTTTCTGAGATCTCT
  GCTGACAAACTGGTGGCTCTGGGGCTGTTCAGCCAGCACTTTAATCTGGCTACCTTCAATAAGCTGGTTT
  CTTATAGGAAGGCCATGTACCACACTCTGGAGAAAGCCAGGGTTCGAGCTGGCAAGACCTTCTCCAGCAG
  TCCTGGAGAGTCACTGGAGGACCAGCTGAAGCCCATGCTGGAGTGGGCCCACGGTGGCTTCAAGCCTACT
  GGGATCGAGGGCCTCAAACCCAACAAGAAGCAACCAGTGGTTAATAAGTCGAAGGTGCGTCGTTCAGACA
  GTAGGAACTTAGAACCCAGGAGACGCGAGAACAAAAGTCGAAGACGCACAACCAATGACTCTGCTGCTTC
  TGAGTCCCCCCCACCCAAGCGCCTCAAGACAAATAGCTATGGCGGGAAGGACCGAGGGGAGGATGAGGAG
  AGCCGAGAACGGATGGCTTCTGAAGTCACCAACAACAAGGGCAATCTGGAAGACCGCTGTTTGTCCTGTG
  GAAAGAAGAACCCTGTGTCCTTCCACCCCCTCTTTGAGGGTGGGCTCTGTCAGAGTTGCCGGGATCGCTT
  CCTAGAGCTCTTCTACATGTATGATGAGGACGGCTATCAGTCCTACTGCACCGTGTGCTGTGAGGGCCGT
  GAACTGCTGCTGTGCAGTAACACAAGCTGCTGCAGATGCTTCTGTGTGGAGTGTCTGGAGGTGCTGGTGG
  GCGCAGGCACAGCTGAGGATGCCAAGCTGCAGGAACCCTGGAGCTGCTATATGTGCCTCCCTCAGCGCTG
  CCATGGGGTCCTCCGACGCAGGAAAGATTGGAACATGCGCCTGCAAGACTTCTTCACTACTGATCCTGAC
  CTGGAAGAATTTCAGGAGCCACCCAAGTTGTACCCAGCAATTCCTGCAGCCAAAAGGAGGCCCATTAGAG
  TCCTGTCTCTGTTTGATGGAATTGCAACGGGGTACTTGGTGCTCAAGGAGTTGGGTATTAAAGTGGAAAA
  GTACATTGCCTCCGAAGTCTGTGCAGAGTCCATCGCTGTGGGAACTGTTAAGCATGAAGGCCAGATCAAA
  TATGTCAATGACGTCCGGAAAATCACCAAGAAAAATATTGAAGAGTGGGGCCCGTTCGACTTGGTGATTG
  GTGGAAGCCCATGCAATGATCTCTCTAACGTCAATCCTGCCCGCAAAGGTTTATATGAGGGCACAGGAAG
  GCTCTTCTTCGAGTTTTACCACTTGCTGAATTATACCCGCCCCAAGGAGGGCGACAACCGTCCATTCTTC
  TGGATGTTCGAGAATGTTGTGGCCATGAAAGTGAATGACAAGAAAGACATCTCAAGATTCCTGGCATGTA
  ACCCAGTGATGATCGATGCCATCAAGGTGTCTGCTGCTCACAGGGCCCGGTACTTCTGGGGTAACCTACC
  CGGAATGAACAGGCCCGTGATGGCTTCAAAGAATGATAAGCTCGAGCTGCAGGACTGCCTGGAGTTCAGT
  AGGACAGCAAAGTTAAAGAAAGTGCAGACAATAACCACCAAGTCGAACTCCATCAGACAGGGCAAAAACC
  AGCTTTTCCCTGTAGTCATGAATGGCAAGGACGACGTTTTGTGGTGCACTGAGCTCGAAAGGATCTTCGG
  CTTCCCTGCTCACTACACGGACGTGTCCAACATGGGCCGCGGCGCCCGTCAGAAGCTGCTGGGCAGGTCC
  TGGAGTGTACCGGTCATCAGACACCTGTTTGCCCCCTTGAAGGACTACTTTGCCTGTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001122997
• ORF Size: 2580 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001122997.1, NM_001122997.2, NP_001116469.1
• RefSeq Size: 4229
• RefSeq ORF: 2583
• Locus ID: 13436
• MW: 97.8 kDa
• Gene Summary: This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene results in severe developmental defects and loss of viability. Mutation of the related gene in humans causes immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. There is a pseudogene for this gene located adjacent to this gene in the same region of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]