Lamb1 (NM_008482) Mouse Tagged ORF Clone

CAT#: MR225668

Lamb1 (Myc-DDK-tagged) - Mouse laminin B1 (Lamb1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lamb1
• Synonyms: C77966; C80098; C81607; D130003D08Rik; Lamb-1; Lamb1-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225668 representing NM_008482
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGCCCCTCTCCTCTCTCCCAACATTTGCCTTTTCTCCCCGCTACCTCTCCAGAAAGGAAGACC
  CGAAGAAAAGACAGGCAGCTTGCCTGCTGCGTCCTCCTTCCCGTGCCGCGTCCCCTCGTCTGCGAGGACT
  GGACATGGGGCTGCTCCAGGTGTTCGCCTTTGGTGTCCTAGCCCTATGGGGCACCCGAGTGTGCGCTCAG
  GAACCGGAGTTCAGCTATGGCTGCGCAGAAGGCAGCTGCTACCCTGCCACTGGCGACCTTCTCATCGGCC
  GAGCGCAAAAGCTCTCCGTGACTTCGACATGTGGACTGCACAAACCAGAGCCCTACTGTATTGTTAGCCA
  CCTGCAGGAGGACAAGAAATGCTTCATATGTGACTCCCGAGACCCTTATCACGAGACCCTCAACCCCGAC
  AGCCATCTCATTGAGAACGTGGTCACCACATTTGCTCCAAACCGCCTTAAGATCTGGTGGCAATCGGAAA
  ATGGTGTGGAGAACGTGACCATCCAACTGGACCTGGAAGCAGAATTCCATTTCACTCATCTCATCATGAC
  CTTCAAGACATTCCGCCCAGCCGCCATGCTGATCGAGCGGTCTTCTGACTTTGGGAAGACTTGGGGCGTG
  TACAGATACTTCGCCTACGACTGTGAGAGCTCGTTCCCAGGCATTTCAACTGGACCCATGAAGAAAGTGG
  ATGACATCATCTGTGACTCTCGATATTCTGACATTGAGCCCTCGACAGAAGGAGAGGTAATATTTCGTGC
  TTTAGATCCTGCTTTCAAAATTGAAGACCCTTATAGTCCAAGGATACAGAATCTATTAAAAATCACCAAC
  TTGAGAATCAAGTTTGTGAAACTGCACACCTTGGGGGATAACCTTTTGGACTCCAGAATGGAAATCCGAG
  AGAAGTACTATTACGCTGTTTATGATATGGTGGTTCGAGGGAACTGCTTCTGCTATGGCCACGCCAGTGA
  ATGCGCCCCTGTGGATGGAGTCAATGAAGAAGTGGAAGGAATGGTTCACGGGCACTGCATGTGCAGACAC
  AACACCAAAGGCCTGAACTGTGAGCTGTGCATGGATTTCTACCACGATTTGCCGTGGAGACCTGCTGAAG
  GCCGGAACAGCAACGCCTGCAAAAAATGTAACTGCAATGAACATTCCAGCTCGTGTCACTTTGACATGGC
  AGTCTTCCTGGCTACTGGCAACGTCAGCGGGGGAGTGTGTGATAACTGTCAGCACAACACCATGGGGCGC
  AACTGTGAACAGTGCAAACCGTTCTACTTCCAGCACCCTGAGAGGGACATCCGGGACCCCAATCTCTGTG
  AACCATGTACCTGTGACCCAGCTGGTTCTGAGAATGGCGGGATCTGTGATGGGTACACTGATTTTTCTGT
  GGGTCTCATTGCTGGTCAGTGTCGGTGCAAATTGCACGTGGAGGGAGAGCGCTGTGATGTTTGTAAAGAA
  GGCTTCTACGACTTAAGTGCTGAAGACCCGTATGGTTGTAAATCATGTGCTTGCAATCCTCTGGGAACAA
  TTCCTGGTGGGAATCCTTGTGATTCTGAGACTGGCTACTGCTACTGTAAGCGCCTGGTGACAGGACAGCG
  CTGTGACCAGTGCCTGCCGCAGCACTGGGGTTTAAGCAATGATTTGGATGGGTGTCGACCTTGTGACTGT
  GACCTTGGAGGGGCGCTGAACAATAGCTGCTCCGAGGACTCCGGCCAGTGCTCCTGCCTGCCCCACATGA
  TTGGGCGGCAGTGTAACGAGGTGGAGTCCGGTTACTACTTCACCACCCTGGACCACTACATCTACGAAGC
  CGAGGAAGCCAATCTGGGGCCTGGAGTCGTTGTGGTGGAAAGGCAGTACATTCAGGACCGCATTCCTTCC
  TGGACAGGACCTGGCTTCGTCCGGGTGCCTGAAGGGGCTTATTTGGAGTTTTTCATTGACAACATACCAT
  ATTCCATGGAGTATGAAATCCTGATTCGCTATGAGCCACAGCTGCCGGACCACTGGGAGAAAGCTGTCAT
  CACTGTACAGCGGCCGGGGAAGATTCCAGCCAGCAGCCGATGTGGTAACACCGTTCCCGATGATGACAAC
  CAGGTGGTGTCCTTGTCACCGGGCTCAAGATACGTTGTCCTCCCTCGCCCCGTGTGCTTTGAGAAGGGAA
  TGAACTACACGGTGAGGTTGGAGCTGCCCCAGTATACGGCATCGGGCAGTGACGTGGAGAGCCCTTACAC
  GTTCATCGACTCGCTTGTTCTCATGCCCTACTGTAAATCGCTGGACATCTTCACTGTTGGCGGCTCAGGC
  GATGGGGAGGTCACCAATAGTGCCTGGGAAACCTTCCAGCGCTACAGGTGTCTGGAGAACAGCAGGAGTG
  TGGTAAAAACACCCATGACAGATGTCTGCAGAAACATTATCTTCAGCATTTCTGCCTTGATTCACCAGAC
  GGGCCTTGCTTGTGAATGTGACCCCCAGGGATCTCTGAGTTCTGTGTGTGACCCCAATGGTGGCCAGTGC
  CAGTGCCGTCCTAATGTGGTTGGAAGAACCTGCAACAGGTGTGCCCCGGGCACCTTTGGCTTTGGCCCCA
  ACGGATGCAAACCTTGTGACTGCCATCTGCAAGGGTCTGCCAGTGCCTTCTGCGATGCGATCACTGGCCA
  GTGCCACTGTTTCCAGGGCATCTATGCTCGGCAGTGTGACCGATGTCTCCCTGGGTATTGGGGCTTTCCC
  AGCTGCCAGCCCTGCCAGTGTAATGGTCATGCTCTAGACTGTGACACAGTGACAGGGGAGTGTCTGAGCT
  GTCAGGACTACACCACGGGCCACAACTGCGAAAGGTGCCTGGCTGGCTACTACGGTGATCCCATCATTGG
  GTCAGGAGACCACTGTCGCCCTTGCCCTTGTCCTGATGGTCCTGACAGTGGACGACAGTTTGCCAGGAGC
  TGTTATCAAGACCCCGTCACTCTCCAGCTTGCGTGTGTTTGTGATCCTGGGTACATTGGCTCCAGATGTG
  ATGACTGTGCCTCTGGATTTTTTGGCAATCCCTCAGACTTTGGGGGTTCATGTCAACCGTGTCAGTGCCA
  CCACAACATTGACACTACCGATCCAGAAGCCTGTGACAAGGACACGGGACGATGCCTCAAGTGCCTGTAC
  CACACGGAAGGGGACCATTGCCAGCTCTGCCAGTATGGGTACTACGGCGATGCTCTTCGGCAAGACTGTA
  GAAAGTGTGTCTGCAATTACCTGGGCACGGTGAAGGAACATTGTAATGGCTCTGACTGCCACTGTGACAA
  AGCCACTGGTCAGTGCTCGTGCCTTCCCAATGTGATCGGGCAGAACTGTGACCGGTGTGCGCCCAACACC
  TGGCAGCTGGCTAGCGGGACTGGCTGCGGGCCCTGCAATTGCAATGCTGCGCATTCCTTTGGGCCATCCT
  GCAACGAGTTCACAGGGCAGTGCCAGTGCATGCCGGGCTTTGGAGGCCGAACCTGCAGCGAGTGCCAGGA
  GCTCTTCTGGGGAGACCCTGATGTGGAATGCCGAGCCTGTGACTGTGATCCCAGGGGCATTGAGACACCT
  CAGTGTGACCAGTCCACGGGCCAGTGTGTCTGTGTGGAGGGTGTAGAGGGTCCTCGCTGCGACAAGTGCA
  CCAGAGGTTACTCGGGGGTCTTTCCTGACTGCACACCCTGCCACCAGTGCTTTGCTCTCTGGGATGCTAT
  CATTGGTGAGCTGACCAACAGGACCCACAAATTCCTGGAGAAAGCCAAGGCTCTGAAAATCAGTGGTGTG
  ATTGGTCCCTACCGAGAGACCGTGGACTCTGTAGAGAAGAAAGTCAATGAGATAAAAGACATCCTGGCCC
  AGAGCCCAGCAGCGGAACCACTGAAAAACATTGGCATTCTCTTCGAGGAGGCAGAGAAACTAACCAAAGA
  TGTCACAGAAAAGATGGCGCAGGTAGAAGTGAAATTAACTGATACAGCTTCACAGAGTAACAGCACAGCT
  GGAGAGCTCGGCGCACTGCAGGCAGAAGCAGAGAGCCTTGACAAGACCGTGAAGGAGCTGGCAGAACAGC
  TGGAGTTTATCAAAAACTCCGATATTCAGGGCGCCTTGGATAGCATCACCAAGTATTTCCAGATGTCTCT
  TGAGGCAGAGAAGCGGGTGAATGCCTCCACCACAGACCCCAACAGCACTGTGGAGCAGTCTGCCCTCACG
  CGAGACAGAGTAGAAGATCTGATGTTGGAGCGAGAGTCTCCGTTCAAGGAGCAGCAGGAGGAACAGGCAC
  GCCTCCTGGACGAACTGGCCGGCAAACTGCAAAGTCTCGACCTGTCGGCTGCTGCACAGATGACCTGTGG
  AACACCTCCAGGGGCTGACTGTTCTGAAAGTGAATGTGGTGGCCCCAACTGCAGAACTGACGAAGGAGAG
  AAGAAGTGTGGGGGGCCTGGCTGTGGTGGTCTGGTCACTGTGGCCCACAGTGCTTGGCAGAAAGCCATGG
  ATTTTGACCGTGATGTCCTGAGTGCCCTGGCTGAAGTCGAACAGCTCTCCAAGATGGTCTCTGAAGCAAA
  AGTGAGAGCAGATGAGGCGAAGCAGAATGCGCAGGATGTCCTGTTAAAAACAAATGCTACCAAAGAAAAA
  GTGGACAAGAGCAACGAGGACCTGCGGAACCTCATCAAGCAGATCAGAAACTTCCTGACTGAGGATAGTG
  CTGATCTAGACAGTATTGAAGCAGTTGCTAATGAAGTACTGAAAATGGAAATGCCTAGCACGCCACAGCA
  GTTACAGAACCTAACAGAAGACATTCGGGAGCGAGTTGAAACCCTCTCTCAAGTAGAGGTTATTTTGCAG
  CAGAGTGCAGCTGACATTGCCAGAGCTGAGCTGTTGCTTGAGGAAGCTAAGAGAGCAAGCAAAAGTGCAA
  CAGATGTTAAAGTCACTGCAGACATGGTGAAGGAAGCATTAGAAGAAGCAGAAAAGGCCCAGGTTGCAGC
  AGAGAAGGCGATTAAACAAGCTGATGAGGATATCCAAGGAACCCAAAACCTGCTAACATCGATTGAATCT
  GAAACGGCAGCTTCTGAGGAAACCCTGACCAACGCCTCCCAGCGCATCAGCAAGCTTGAGAGGAACGTGG
  AAGAGCTTAAGCGTAAAGCTGCCCAGAACTCTGGGGAGGCAGAATATATCGAAAAAGTAGTATATTCTGT
  AAAACAGAATGCAGACGATGTTAAGAAGACTCTAGATGGCGAACTTGATGAAAAGTATAAGAAGGTAGAA
  AGTTTAATTGCCCAAAAAACTGAAGAGTCAGCAGATGCCAGGAGGAAAGCTGAGCTGCTACAAAATGAAG
  CAAAAACACTCTTGGCTCAAGCTAACAGCAAGCTCCAGCTGTTGGAAGACTTAGAAAGAAAATATGAGGA
  CAATCAAAAATACTTAGAAGATAAAGCTCAAGAATTGGTGCGACTGGAAGGAGAGGTTCGCTCCCTCCTT
  AAGGACATAAGTGAGAAAGTTGCGGTTTACAGCACCTGCTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225668 representing NM_008482
  Red=Cloning site Green=Tags(s)
  
  MEGPLSSLPTFAFSPRYLSRKEDPKKRQAACLLRPPSRAASPRLRGLDMGLLQVFAFGVLALWGTRVCAQ
  EPEFSYGCAEGSCYPATGDLLIGRAQKLSVTSTCGLHKPEPYCIVSHLQEDKKCFICDSRDPYHETLNPD
  SHLIENVVTTFAPNRLKIWWQSENGVENVTIQLDLEAEFHFTHLIMTFKTFRPAAMLIERSSDFGKTWGV
  YRYFAYDCESSFPGISTGPMKKVDDIICDSRYSDIEPSTEGEVIFRALDPAFKIEDPYSPRIQNLLKITN
  LRIKFVKLHTLGDNLLDSRMEIREKYYYAVYDMVVRGNCFCYGHASECAPVDGVNEEVEGMVHGHCMCRH
  NTKGLNCELCMDFYHDLPWRPAEGRNSNACKKCNCNEHSSSCHFDMAVFLATGNVSGGVCDNCQHNTMGR
  NCEQCKPFYFQHPERDIRDPNLCEPCTCDPAGSENGGICDGYTDFSVGLIAGQCRCKLHVEGERCDVCKE
  GFYDLSAEDPYGCKSCACNPLGTIPGGNPCDSETGYCYCKRLVTGQRCDQCLPQHWGLSNDLDGCRPCDC
  DLGGALNNSCSEDSGQCSCLPHMIGRQCNEVESGYYFTTLDHYIYEAEEANLGPGVVVVERQYIQDRIPS
  WTGPGFVRVPEGAYLEFFIDNIPYSMEYEILIRYEPQLPDHWEKAVITVQRPGKIPASSRCGNTVPDDDN
  QVVSLSPGSRYVVLPRPVCFEKGMNYTVRLELPQYTASGSDVESPYTFIDSLVLMPYCKSLDIFTVGGSG
  DGEVTNSAWETFQRYRCLENSRSVVKTPMTDVCRNIIFSISALIHQTGLACECDPQGSLSSVCDPNGGQC
  QCRPNVVGRTCNRCAPGTFGFGPNGCKPCDCHLQGSASAFCDAITGQCHCFQGIYARQCDRCLPGYWGFP
  SCQPCQCNGHALDCDTVTGECLSCQDYTTGHNCERCLAGYYGDPIIGSGDHCRPCPCPDGPDSGRQFARS
  CYQDPVTLQLACVCDPGYIGSRCDDCASGFFGNPSDFGGSCQPCQCHHNIDTTDPEACDKDTGRCLKCLY
  HTEGDHCQLCQYGYYGDALRQDCRKCVCNYLGTVKEHCNGSDCHCDKATGQCSCLPNVIGQNCDRCAPNT
  WQLASGTGCGPCNCNAAHSFGPSCNEFTGQCQCMPGFGGRTCSECQELFWGDPDVECRACDCDPRGIETP
  QCDQSTGQCVCVEGVEGPRCDKCTRGYSGVFPDCTPCHQCFALWDAIIGELTNRTHKFLEKAKALKISGV
  IGPYRETVDSVEKKVNEIKDILAQSPAAEPLKNIGILFEEAEKLTKDVTEKMAQVEVKLTDTASQSNSTA
  GELGALQAEAESLDKTVKELAEQLEFIKNSDIQGALDSITKYFQMSLEAEKRVNASTTDPNSTVEQSALT
  RDRVEDLMLERESPFKEQQEEQARLLDELAGKLQSLDLSAAAQMTCGTPPGADCSESECGGPNCRTDEGE
  KKCGGPGCGGLVTVAHSAWQKAMDFDRDVLSALAEVEQLSKMVSEAKVRADEAKQNAQDVLLKTNATKEK
  VDKSNEDLRNLIKQIRNFLTEDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVETLSQVEVILQ
  QSAADIARAELLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAIKQADEDIQGTQNLLTSIES
  ETAASEETLTNASQRISKLERNVEELKRKAAQNSGEAEYIEKVVYSVKQNADDVKKTLDGELDEKYKKVE
  SLIAQKTEESADARRKAELLQNEAKTLLAQANSKLQLLEDLERKYEDNQKYLEDKAQELVRLEGEVRSLL
  KDISEKVAVYSTCL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008482
• ORF Size: 5502 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008482.1, NM_008482.2, NP_032508.2
• RefSeq Size: 5778
• RefSeq ORF: 5361
• Locus ID: 16777
• MW: 202.9 kDa