Arhgap35 (NM_172739) Mouse Tagged ORF Clone

CAT#: MR225830

Grlf1 (Myc-DDK-tagged) - Mouse glucocorticoid receptor DNA binding factor 1 (Grlf1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgap35
• Synonyms: 6430596G11Rik; AI841135; Grlf1; mKIAA1722; p190A; p190RhoGAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225830 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGATGGCAAGAAAGCAAGATGTCCGCATCCCCACCTACAACATCAGTGTGGTGGGATTGTCAGGCA
  CTGAGAAGGAGAAAGGCCAGTGCGGCATTGGGAAGTCTTGTCTGTGTAACCGCTTTGTGCGCCCAAGTGC
  TGATGAGTTTCACCTGGACCACACTTCTGTCCTTAGCACCAGTGACTTTGGTGGGCGGGTGGTCAATAAT
  GACCACTTTCTGTACTGGGGAGAAGTTAGCCGCTCCCTGGAGGATTGTGTGGAATGTAAGATGCACATTG
  TGGAGCAGACTGAGTTTATTGACGATCAGACTTTTCAACCTCATCGAAGCACGGCACTGCAGCCCTACAT
  CAAGAGAGCCGCAGCCACCAAGCTTGCTTCAGCTGAAAAACTCATGTATTTTTGCACTGACCAGCTGGGA
  CTGGAGCAAGACTTTGAGCAGAAACAGATGCCAGATGGGAAGCTGCTGGTTGACGGTTTCCTTCTGGGCA
  TTGATGTTAGCAGGGGCATGAACAGGAACTTCGATGATCAGCTCAAATTTGTCTCCAATCTCTACAATCA
  GCTTGCAAAAACAAAAAAGCCCATAGTGGTAGTCCTGACTAAGTGTGATGAGGGCGTTGAGCGGTACATT
  AGAGATGCACATACTTTTGCCTTAAGCAAAAAGAACCTCCAGGTTGTAGAGACCTCTGCAAGGTCCAATG
  TGAATGTGGACTTGGCTTTCAGCACCTTAGTGCAACTCATTGATAAGAGTCGAGGGAAGACAAAAATCAT
  CCCTTACTTTGAAGCTCTCAAGCAGCAGAGTCAGCAGATAGCTACAGCAAAGGACAAGTATGAGTGGTTA
  GTGAGCCGCATTGTGAAAAATCACAATGAGAACTGGCCGAGTGTCAGCCGAAAGATGCAGGCCTCCCCTG
  AGTACCAGGACTATGTCTACCTGGAAGGGACACAGAAGGCCAAGAAGCTCTTCCTGCAGCACATTCACCG
  CCTCAAGCATGAGCATATTGAGCGTCGGAGAAAGCTGTACCTGGCAGCTCTGCCATTGGCTTTTGAAGCC
  CTCATACCTAATCTAGATGAAGTGGACCACCTGAGCTGTATTAAAGCAAAAAAGCTGTTAGAGACCAAGC
  CAGAGTTCTTAAAGTGGTTTGTTGTACTTGAAGAAACACCATGGGATGCCACCAGCCACATTGACAACAT
  GGAAAATGAGCGGATTCCCTTTGACTTGATGGATACTGTCCCTGCTGAGCAGTTGTATGAGACCCACTTG
  GAGAAGCTGAGGAACGAGAGGAAGAGAGCTGAGATGCGAAGGGCTTTCAAAGAAAACCTGGAGACCTCTC
  CTTTCATAACTCCTGGGAAACCTTGGGAAGAAGCTCGTAGTTTTATTATGAATGAAGACTTCTACCAGTG
  GCTGGAAGAATCTGTGTACATGGACATCTATGGCAAGCACCAAAAGCAGATTATAGACCGAGCAAAGGAA
  GAGTTCCAAGAGTTGCTTTTGGAGTACTCAGAATTGTTTTATGAGCTGGAGCTGGACGCTAAGCCCAGCA
  AGGAGAAGATGGGTGTCATCCAGGATGTTCTGGGCGAAGAGCAGAGATTTAAAGCATTGCAGAAGCTCCA
  AGCAGAGCGTGATGCCCTCATTCTGAAGCACATCCATTTTGTGTACCACCCGACGAAGGAGACATGCCCA
  AGCTGTCCAGCTTGTGTAGATGCTAAGATTGAACACTTGATCAGTTCTCGCTTTATCAGACCATCTGATC
  GGAATCAGAAGAACTCTCTGTCTGACCTCAATATTGATAGGATCAATTTGGTTATCTTAGGCAAAGATGG
  CCTTGCCCGAGAGTTGGCCAATGAAATTCGAGCTCTTTGTACAAATGATGACAAGTATGTAATAGATGGT
  AAAATGTATGAGCTTTCTCTGAGGCCAATAGAGGGGAATGTCCGGCTTCCTGTGAACTCTTTCCAGACAC
  CAACCTTCCAACCCCATGGCTGTCTCTGCCTTTACAATTCAAAGGAGTCACTGTCCTATGTGGTGGAGAG
  TATAGAGAAGAGCAGAGAGTCTACACTGGGCAGGCGGGATAATCATTTAGTCCACCTTCCCTTGACCTTA
  ATTTTAGTTAACAAGAGAGGGGACACAAGTGGAGAGACTCTGCACAGCTTGATACAGCAAGGTCAGCAGA
  TTGCCAGCAAACTTCAGTGTGTCTTTCTTGATCCGGCTTCTGCTGGCATTGGTTATGGACGCAACATTAA
  TGAGAAGCAGATCAGTCAAGTTCTGAAAGGACTCCTGGACTCCAAGCGCAACTTAAACCTGGTTAGCTCC
  ACTGCAAGTATCAAAGATTTGGCTGATGTGGACCTTCGAATTGTCATGTGTCTGATGTGTGGTGATCCCT
  TTAGTGCAGATGACGTTCTCTCTCCTGTCCTGCAATCCCAAACTTGTAAATCTTCCCACTGTGGGAGCAG
  CAACTCTGTTTTACTTGAACTTCCAATTGGACTACACAAGAAGCGCATTGAGCTGTCTGTTCTTTCATAC
  CATTCCTCATTTAGCATCCGAAAGAGCCGGTTGGTTCATGGGTACATTGTTTTTTATTCAGCCAAACGTA
  AGGCCTCCTTGGCAATGTTACGTGCCTTTCTTTGTGAAGTGCAGGATATTATCCCCATCCAGCTTGTCGC
  ACTCACTGATGGCGCTATAGATGTCCTAGACAATGACTTAAGTCGAGAGCAGCTAACAGAGGGAGAGGAA
  ATTGCACAAGAGATTGACGGGAGATTCACAAGCATCCCTTGTAGCCAGCCCCAGCATAAACTTGAGCTCT
  TTCATCCCTTTTTTAAAGATGTGGTGGAGAAAAAGAACATAATTGAGGCCACACATATGTATGATAATGT
  AGCTGAGGCCTGCAGCACCACTGAGGAAGTGTTCAACTCCCCCAGAGCTGGCTCACCCCTCTGCAACTCA
  AACTTACAGGACTCAGAAGAAGATGTGGAGCCTCCATCATACCACCTGTTTCGGGAAGATGCGACATTGC
  CCTCCCTGTCCAAAGATCATTCCAAGTTCTCTATGGAACTGGAGGGAAATGACGGGCTATCTTTCATAAT
  GAGCAATTTTGAGAGTAAACTGAACAACAAAGTACCTCCCCCAGTCAAACCAAAGCCTCCTGTGCATTTT
  GATATCACAAAAGATCTTTCTTACTTAGACCAAGGCCATCGGGAGGGACAGAGGAAATCTATGTCTTCTA
  GCCCCTGGATGCCTCAGGATGGGTTTGATCCTTCTGACTACGCAGAGCCCATGGATGCTGTGGTCAAGCC
  AAGGAATGAGGAAGAAAACATATACTCGGTGCCCCACGACAGCACCCAGGGCAAGATCATTACCATTCGG
  AACATCAACAAAGCCCAGTCCAATGGCAGTGGTAATGGTTCTGACAGTGAGATGGACACAAGTTCCCTAG
  AGCGAGGCCGCAAGGTATCTGCAGTGAGTAAGCCTGTGCTGTACAGGACAAGATGCACTCGCCTGGGGCG
  GTTTGCTAGTTACCGTACCAGCTTCAGTGTCGGGAGTGATGATGAGCTGGGACCCATCCGAAAGAAAGAG
  GAGGACCAGGCATCCCAAGGTTATAAAGGGGACAATGCTGTCATTCCATATGAAACAGATGAGGACCCCA
  GGAGGAGGAATATCCTTCGAAGTCTAAGAAGGAACACCAAGAAACCAAAGCCCAAACCCCGGCCATCCAT
  CACAAAGGCAACCTGGGAGAGTAACTATTTTGGGGTGCCCTTAACAACAGTGGTGACTCCAGAGAAACCG
  ATACCCATTTTCATTGAAAGATGCATTGAGTACATCGAAGCCACAGGACTAAGCACTGAAGGCATCTACC
  GGGTCAGTGGAAACAAGTCAGAAATGGAAAGTTTGCAAAGACAGTTTGATCAAGACCACAATCTGGACCT
  GGCAGAGAAAGACTTCACTGTGAACACTGTGGCAGGGGCCATGAAGAGTTTTTTCTCAGAGCTACCAGAC
  CCCCTGGTACCATACAGCATGCAGATTGACTTGGTGGAAGCTCACAAAATCAACGACAGGGAACAGAAGC
  TGCATGCTCTGAAGGAAGTGCTGAAGAAGTTCCCGAAGGAAAACCATGAAGTCTTCAAATATGTCATCTC
  CCACCTGAACAAAGTCAGCCACAACAACAAGGTGAATCTTATGACCAGTGAGAACCTGTCCATCTGCTTC
  TGGCCCACGCTGATGCGGCCTGACTTCAGCAGCATGGACGCGCTCACAGCCACGCGCTCCTACCAGACCA
  TCATCGAGCTCTTCATCCAGCAGTGCCCCTTCTTCTTCTACAACCGGCCCATCAGCGAGCCTCCAGGGGC
  TGCGCCTGGCTCCCCTTCAGCCATGGCTCCCACTGTTCCCTTCCTCACCTCTACACCTGCTACCAGTCAG
  CCATCACCTCCCCAGTCACCTCCTCCAACCCCTCAGTCCCCAATGCAGCCATTGCTGTCCTCTCAGCTCC
  AAGCCGAACACACGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225830 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNN
  DHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLG
  LEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVVVLTKCDEGVERYI
  RDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWL
  VSRIVKNHNENWPSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEA
  LIPNLDEVDHLSCIKAKKLLETKPEFLKWFVVLEETPWDATSHIDNMENERIPFDLMDTVPAEQLYETHL
  EKLRNERKRAEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDRAKE
  EFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCP
  SCPACVDAKIEHLISSRFIRPSDRNQKNSLSDLNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDG
  KMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTL
  ILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSS
  TASIKDLADVDLRIVMCLMCGDPFSADDVLSPVLQSQTCKSSHCGSSNSVLLELPIGLHKKRIELSVLSY
  HSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAIDVLDNDLSREQLTEGEE
  IAQEIDGRFTSIPCSQPQHKLELFHPFFKDVVEKKNIIEATHMYDNVAEACSTTEEVFNSPRAGSPLCNS
  NLQDSEEDVEPPSYHLFREDATLPSLSKDHSKFSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHF
  DITKDLSYLDQGHREGQRKSMSSSPWMPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIR
  NINKAQSNGSGNGSDSEMDTSSLERGRKVSAVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKE
  EDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKP
  IPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPD
  PLVPYSMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNKVSHNNKVNLMTSENLSICF
  WPTLMRPDFSSMDALTATRSYQTIIELFIQQCPFFFYNRPISEPPGAAPGSPSAMAPTVPFLTSTPATSQ
  PSPPQSPPPTPQSPMQPLLSSQLQAEHTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172739
• ORF Size: 4500 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_172739.3, NM_172739.4, NP_766327.3
• RefSeq Size: 8393
• RefSeq ORF: 4500
• Locus ID: 232906
• MW: 170.4 kDa