Shc1 (NM_001113331) Mouse Tagged ORF Clone

CAT#: MR225873

Shc1 (Myc-DDK-tagged) - Mouse src homology 2 domain-containing transforming protein C1 (Shc1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Shc1
• Synonyms: p66; p66shc; Shc; ShcA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225873 representing NM_001113331
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTTCTACCCCCCAAGCCGAAGTACAACCCACTTCGGAATGAGTCTCTGTCATCGCTGGAGGAGG
  GGGCTTCGGGGTCTACCCCTCCGGAGGAGCTACCTTCCCCATCAGCTTCATCCCTGGGACCCATTCTGCC
  TCCTCTGCCGGGGGACGATAGTCCGACTACCCTGTGTTCCTTCTTTCCCCGGATGAGCAACCTGAAGCTG
  GCCAATCCTGCTGGGGGGCGCCTGGGGCCTAAAGGGGAGCCAGGAAAGGCTGCTGAAGATGGGGAAGGGA
  GTGCAGGGGCAGCCCTTCGGGACTCAGGCCTCTTGCCCCTCCTCCAGGACATGAACAAGCTGAGTGGAGG
  CGGCGGGCGCAGGACTCGGGTAGAAGGGGGCCAGCTGGGGGGCGAGGAGTGGACCAGACACGGGAGCTTT
  GTCAATAAGCCCACACGAGGCTGGCTGCATCCCAACGACAAAGTCATGGGACCTGGGGTTTCCTACTTGG
  TTCGGTACATGGGCTGTGTGGAGGTCTTACAGTCAATGCGAGCCCTTGACTTCAATACCCGGACTCAGGT
  CACCAGGGAGGCCATCAGTTTGGTGTGTGAAGCTGTGCCTGGTGCCAAAGGGGCGACAAGGAGGAGAAAG
  CCTTGTAGCCGCCCACTCAGCTCCATCCTGGGGAGGAGTAACCTGAAGTTTGCTGGAATGCCAATCACTC
  TCACTGTGTCTACCAGCAGCCTTAACCTCATGGCAGCCGACTGCAAACAGATCATTGCCAACCATCACAT
  GCAATCTATCTCTTTCGCGTCCGGTGGGGATCCGGACACAGCTGAGTATGTTGCCTATGTTGCCAAAGAC
  CCTGTGAATCAGAGAGCCTGCCATATCCTGGAGTGTCCTGAAGGGCTTGCTCAGGATGTCATCAGCACCA
  TCGGGCAGGCCTTTGAGTTGCGCTTCAAACAGTATCTCAGGAATCCACCGAAGCTGGTCACCCCCCATGA
  CAGGATGGCTGGCTTTGATGGCTCAGCTTGGGATGAGGAGGAAGAAGAGCCCCCTGACCATCAGTACTAC
  AATGACTTTCCAGGGAAGGAACCCCCTCTTGGTGGGGTGGTAGATATGAGGCTTCGGGAAGGGGCTGCTC
  GACCCACTCTGCCTAGTGCCCAGATGTCCAGCCACTTGGGAGCTACACTGCCTATAGGGCAGCATGCTGC
  AGGAGACCATGAAGTCCGTAAACAGATGTTGCCTCCGCCGCCTTGCCCAGGCAGAGAACTCTTCGATGAC
  CCCTCCTATGTCAACATCCAGAATCTAGACAAGGCCCGGCAGGCTGGGGGTGGGGCTGGGCCCCCAAATC
  CTTCTCTTAATGGCAGTGCACCCCGAGACCTTTTTGACATGAAGCCCTTTGAAGATGCACTTCGGGTGCC
  ACCCCCACCGCAGTCCATGTCCATGGCTGAGCAGCTGCAAGGGGAGCCCTGGTTCCACGGGAAGCTGAGC
  CGGAGGGAGGCCGAGGCGCTGCTGCAGCTCAATGGTGACTTCTTGGTGCGAGAGAGCACGACCACGCCTG
  GCCAGTATGTGCTCACTGGCCTGCAGAGTGGGCAGCCCAAGCACTTGCTGCTGGTGGACCCTGAAGGTGT
  GGTTCGGACAAAGGATCACCGCTTTGAGAGTGTCAGTCACCTGATCAGCTACCACATGGACAATCACTTG
  CCCATCATCTCTGCGGGCAGCGAACTGTGCCTACAGCAACCCGTGGATCGGAAAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225873 representing NM_001113331
  Red=Cloning site Green=Tags(s)
  
  MDLLPPKPKYNPLRNESLSSLEEGASGSTPPEELPSPSASSLGPILPPLPGDDSPTTLCSFFPRMSNLKL
  ANPAGGRLGPKGEPGKAAEDGEGSAGAALRDSGLLPLLQDMNKLSGGGGRRTRVEGGQLGGEEWTRHGSF
  VNKPTRGWLHPNDKVMGPGVSYLVRYMGCVEVLQSMRALDFNTRTQVTREAISLVCEAVPGAKGATRRRK
  PCSRPLSSILGRSNLKFAGMPITLTVSTSSLNLMAADCKQIIANHHMQSISFASGGDPDTAEYVAYVAKD
  PVNQRACHILECPEGLAQDVISTIGQAFELRFKQYLRNPPKLVTPHDRMAGFDGSAWDEEEEEPPDHQYY
  NDFPGKEPPLGGVVDMRLREGAARPTLPSAQMSSHLGATLPIGQHAAGDHEVRKQMLPPPPCPGRELFDD
  PSYVNIQNLDKARQAGGGAGPPNPSLNGSAPRDLFDMKPFEDALRVPPPPQSMSMAEQLQGEPWFHGKLS
  RREAEALLQLNGDFLVRESTTTPGQYVLTGLQSGQPKHLLLVDPEGVVRTKDHRFESVSHLISYHMDNHL
  PIISAGSELCLQQPVDRKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113331
• ORF Size: 1737 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001113331.1, NM_001113331.2, NP_001106802.1
• RefSeq Size: 3569
• RefSeq ORF: 1740
• Locus ID: 20416
• MW: 63.1 kDa