Nova2 (NM_001029877) Mouse Tagged ORF Clone

CAT#: MR225990

Nova2 (Myc-DDK-tagged) - Mouse neuro-oncological ventral antigen 2 (Nova2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nova2
• Synonyms: Gm1424
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225990 representing NM_001029877
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGAGGCCCCGGATTCCCGCAAGAGACCCCTCGAGACGCCCCCCGAGGTGGTCTGCACCAAGC
  GCAGCAACACGGGAGAGGAAGGCGAGTACTTCCTGAAGGTGCTCATCCCCAGCTACGCCGCCGGCTCAAT
  CATCGGCAAAGGTGGCCAGACCATCGTGCAGCTCCAGAAGGAGACAGGAGCCACCATCAAGCTGTCTAAG
  TCCAAAGACTTCTACCCAGGAACTACAGAACGGGTATGCCTAGTACAGGGCACGGCAGAGGCCTTGAATG
  CTGTCCACAGCTTTATCGCAGAAAAGGTCCGAGAAATCCCTCAAGCGATGACCAAGCCTGAGGTGGTCAA
  CATCCTTCAACCCCAAACCACGATGAACCCCGACAGAGCCAAGCAGGCCAAGCTCATCGTCCCCAACAGC
  ACGGCAGGACTGATCATCGGTAAGGGAGGAGCGACCGTGAAGGCGGTGATGGAACAGTCCGGCGCGTGGG
  TGCAGCTGTCGCAGAAGCCCGAGGGCATCAACCTGCAAGAGCGCGTGGTGACCGTGAGCGGCGAGCCGGA
  GCAGGTGCACAAGGCCGTGAGCGCCATCGTGCAGAAGGTACAGGAAGACCCGCAGAGCAGCAGCTGCCTC
  AACATCAGCTACGCCAACGTGGCGGGGCCCGTGGCCAACTCCAACCCCACCGGCTCGCCCTACGCCAGCC
  CGGCCGACGTGCTCCCCGCCGCGGCCGCCGCCTCTGCCGCCGCCGCCTCGGGTCTGCTAGGCCCCGCGGG
  GCTGGCGGGCGTGGGCGCCTTCCCCGCCGCGCTGCCCGCCTTCTCGGGCACCGACCTGCTGGCCATCAGC
  ACGGCGCTCAACACGTTGGCCAGCTACGGCTACAACACCAACTCGCTCAGCCTGGGCCTCAACTCGGCCG
  CCGCCTCCGGCGTCCTGGCCGCCGTAGCCGCAGGCGCCAACCCCGCCGCCGCCGCAGCAGCCAACCTCCT
  GGCGTCCTACGCGGGGGACGCCGGGGCGGGGCCGGGGGCGGGGGCAGCACCGCCACCGCCACCGCCCCCC
  GGAGCGCTGGGTTCCTTCGCTCTGGCCGCGGCTGCCAATGGCTACCTCGGTGCGGGCGCGGGGGGCGCGG
  CTGGAGCGGGGGGCGCGCCCCTGGTGGCCGCCGCGGCTGCGGCCGGGGCTGCCGGCGGCTTCCTGACGGC
  CGAGAAGCTGGCGGCCGAGAGCGCCAAGGAGCTGGTGGAGATCGCGGTGCCCGAGAACCTGGTGGGGGCC
  ATCCTGGGCAAAGGGGGCAAGACGCTGGTGGAGTACCAGGAGCTGACGGGCGCGCGCATCCAGATCTCCA
  AGAAGGGCGAGTTCCTGCCCGGCACGCGGAACCGGCGGGTCACCATCACGGGCAGCCCCGCGGCCACGCA
  AGCCGCTCAATACCTCATCAGCCAGCGGGTCACCTACGAGCAGGGGGTGAGGGCTTCAAACCCACAGAAA
  GTGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225990 representing NM_001029877
  Red=Cloning site Green=Tags(s)
  
  MEPEAPDSRKRPLETPPEVVCTKRSNTGEEGEYFLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSK
  SKDFYPGTTERVCLVQGTAEALNAVHSFIAEKVREIPQAMTKPEVVNILQPQTTMNPDRAKQAKLIVPNS
  TAGLIIGKGGATVKAVMEQSGAWVQLSQKPEGINLQERVVTVSGEPEQVHKAVSAIVQKVQEDPQSSSCL
  NISYANVAGPVANSNPTGSPYASPADVLPAAAAASAAAASGLLGPAGLAGVGAFPAALPAFSGTDLLAIS
  TALNTLASYGYNTNSLSLGLNSAAASGVLAAVAAGANPAAAAAANLLASYAGDAGAGPGAGAAPPPPPPP
  GALGSFALAAAANGYLGAGAGGAAGAGGAPLVAAAAAAGAAGGFLTAEKLAAESAKELVEIAVPENLVGA
  ILGKGGKTLVEYQELTGARIQISKKGEFLPGTRNRRVTITGSPAATQAAQYLISQRVTYEQGVRASNPQK
  VG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001029877
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001029877.2, NM_001029877.3, NP_001025048.2
• RefSeq Size: 8372 bp
• RefSeq ORF: 1479 bp
• Locus ID: 384569
• Cytogenetics: 7 A3
• MW: 49.5 kDa