Btla (NM_001037719) Mouse Tagged ORF Clone

CAT#: MR226023

Btla (Myc-DDK-tagged) - Mouse B and T lymphocyte associated (Btla), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Btla
• Synonyms: A630002H24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226023 representing NM_001037719
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACAGTGCCTGCCATGCTTGGGACTCCTCGGTTATTTAGGGAATTCTTCATCCTCCATCTGGGCC
  TCTGGAGCATCCTTTGTGAGAAAGCTACTAAGAGGAATGATGAAGAGTGTCCAGTGCAACTTACTATTAC
  GAGGAATTCCAAACAGTCTGCCAGGACAGGAGAGTTATTTAAAATTCAATGTCCTGTGAAATACTGTGTT
  CATAGACCTAATGTGACTTGGTGTAAGCACAATGGAACAATCTGTGTACCCCTTGAGGTTAGCCCTCAGC
  TATACACTAGTTGGGAAGAAAATCAATCAGTTCCGGTTTTTGTTCTCCACTTTAAACCAATACATCTCAG
  TGATAATGGGTCGTATAGCTGTTCTACAAACTTCAATTCTCAAGTTATTAATAGCCATTCAGTAACCATC
  CATGTGACAGAAAGGACTCAAAACTCTTCAGAACACCCACTAATAACAGTATCTGACATCCCAGATGCCA
  CCAATGCCTCAGGACCATCCACCATGGAAGAGAGGCCAGGCAGGACTTGGCTGCTTTACACCTTGCTTCC
  TTTGGGGGCATTGCTTCTGCTCCTTGCCTGTGTCTGCCTGCTCTGCTTTCTGAAAAGGATCCAAGGGAAA
  GAAAAGAAGCCTTCTGACTTGGCAGGAAGGGACACTAACCTGGTTGATATTCCAGCCAGTTCCAGGACAA
  ATCACCAAGCACTGCCATCAGGAACTGGAATTTATGATAATGATCCCTGGTCTAGCATGCAGGATGAATC
  TGAATTGACAATTAGCTTGCAATCAGAGAGAAACAACCAGGGCATTGTTTATGCTTCTTTGAACCATTGT
  GTTATTGGAAGGAATCCAAGACAGGAAAACAACATGCAGGAGGCACCCACAGAATATGCATCCATTTGTG
  TGAGAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226023 representing NM_001037719
  Red=Cloning site Green=Tags(s)
  
  MKTVPAMLGTPRLFREFFILHLGLWSILCEKATKRNDEECPVQLTITRNSKQSARTGELFKIQCPVKYCV
  HRPNVTWCKHNGTICVPLEVSPQLYTSWEENQSVPVFVLHFKPIHLSDNGSYSCSTNFNSQVINSHSVTI
  HVTERTQNSSEHPLITVSDIPDATNASGPSTMEERPGRTWLLYTLLPLGALLLLLACVCLLCFLKRIQGK
  EKKPSDLAGRDTNLVDIPASSRTNHQALPSGTGIYDNDPWSSMQDESELTISLQSERNNQGIVYASLNHC
  VIGRNPRQENNMQEAPTEYASICVRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037719
• ORF Size: 918 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037719.2, NP_001032808.2
• RefSeq Size: 3235 bp
• RefSeq ORF: 921 bp
• Locus ID: 208154
• Cytogenetics: 16 B5
• MW: 34.7 kDa
• Gene Summary: Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response. [UniProtKB/Swiss-Prot Function]