Ank2 (NM_001034168) Mouse Tagged ORF Clone

CAT#: MR226071

Ank2 (Myc-DDK-tagged) - Mouse ankyrin 2, brain (Ank2), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ank2
• Synonyms: 100043364; AI835472; Ank-2; AW491075; Gm4392
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226071 representing NM_001034168
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGACAGGCAGTCACAGGGCACCACTCCTGATACCACTCCTGCTAGGACCCCAACTGAAGAGGGGA
  CTCCGACAAGTGAACAAAATCCATTCCTCTTTCAGGAAGGAAAACTGTTTGAGATGACCCGAAGTGGTGC
  TATCGATATGACCAAAAGGCCCTATGCAGATGAAAGTTTGCACTTTTTCCAAATCGGCCAAGAGTCCAAT
  GAAGAGGCTATCTCCGAAGACTTGAAGGAAGGGGCCACTGGGGCTGAGCCTCCACAGACGGAGACTACTA
  GTGAGTCGCTGGAACTTTCAGAACCCAAAGAAGCAATGGATGACGAAGGAGAATTACTTCCTGATGATGT
  AAGTGAGGAGATAGAGGATTTACCTGCCTCGGATGCTAACATTGACTCCCAAGTGATAATTTCAGCTTCC
  ACAGAAACACCCACCAAAGAGGCTGTATCCACAGCGGTCGAGGAGCCCCCCACCACACAGCGGAGTGATT
  CTCTGAGCACTGTGAAGCAGACACCACGCCCTGCCGTCCCTGGACCTGTTGGTCAGTTGGACTTTTCCCC
  CGTCACTAGGTCTGTTTATTCGGGACAGGATGATGAGTCCCCAGAGTCTTCTCCAGAGGAACAGAAGTCT
  GTGATTGAGATCCCTACTGCACCCGTGGACAACGTGCCTTCTGCCGAAAGCAAACCCCAAATTCCTATCA
  GGACTCTCCCCACTTTAGTCCCAGCCCCTCCATCTGCAGAGGATGAGAGTGCATTTTCTGATGATTTCCC
  ATCTAGCCTGGATGAGGATAGTAAGGAAGGTGGAGCAAAACCAAAGTCCAAAATTCCCGTCAAAGCACCC
  ACCCAAAGAACTGAGTGGCAGCCCTCCCCTACCGACATACCTCTCCAGAAGACAGCTGTCCCCCAGGGAC
  AGGAAACACTAAGCAGAGCACCAGATGGTAGAAGCAAGTCAGAGTCAGACGCTAGTTCCTTAGATGCTAA
  GACCAAATGCCCAGTGAAAGCCAGAAGTTACATTGAGACAGAGACGGAGAGCAGGGAGAGGGCCGAGGGG
  TTTGAGTCAGAATCAGAAGACGGGGCCACAAAACCAAAGCTCTTTGCATCCCGACTGCCGGTGAAGAGCA
  GGAGCACTTCATCTTCCGGCAGGCCAGGCACGAGCCCCACCAGAGAGAGCAGGGAGCACTTCTTTGACCT
  TTACCGAAACTCCATAGAATTCTTTGAGGAGATTAGTGATGAAGCTTCCAAATTAGTGGACAGGCTTACA
  CAGTCAGAGAGGGAGCAGGAGCCACCTTCAGACGACGAAAGTAGCAGTGCCCTGGAAGTGTCAGTCATTG
  AGAGTCTGCCACCTGTTGACATTGAGCACTCAGCTCCCGAGGACATCTTTGACACAAGGCCCATTTGGGA
  TGAGTCTATTGAGACTATGATTGAACGCATCCCTGATGAAAATGGCCATGACCGAGCTGAAGATCCCCAA
  GATGAGCAGGAGCGGATGGAAGAAAGGCTGGCTTACATCGCTGATCACCTTGGCTTCAGCTGGACAGAAT
  TAGCAAGAGAACTGGATTTCACTGAGGAGCAAATTCACCAAATTCGAATCGAGAACCCCAACTCCCTTCA
  AGATCAGAGCCACGCACTGCTCAAGTACTGGCTGGAGAGGGATGGGAAGCATGCCACAGATACCATCCTC
  ATCGAATGCCTCACCAAGATCAACAGGATGGACATTGTACATCTCCTGGAGACCAACACAGAGCCCCTCC
  AGGAGCGCATGGGCCGCAGCTATGCAGAAATAGAGCAGACCATTACGCTGGACCACAGTGAAGGATTTTC
  AGTCCTTCCAGACGAGCTCTGTGCTGCCAAGGAGAAGAAGGAGCAGGAAGCTTCCAAAGAAAGCGAGTCT
  AGCGACCACCCGCCCATGGTCTCCGAAGAAGACATATCTGTCGGTTATTCCACATTTCAGGATTGCCTCC
  CCAAAACTGAAGGGGACAGCCCAGCAGCAGCACTGTCTCCTCAAATGCACCAGGAGCCAGTTCAACAAGA
  TTTCTCAGGGAAAACGCAAGACCAGCAGGAATATTATGTAACAACACCAGGGGCAGAAGTGGAAGACCCT
  CAGAAGGCCACAGCCGTTCCTGACTCTCTCTGTAAGACTCCTGAGGACATCAGTACCCCTCCTGAGGGAA
  CAAAGCCTTGTCTCCAGACCCCGGTGACTAGCGAACGTGGTTCTCCGATTGTGCAAGAACCTGAGGAGGC
  TTCCGAGCCCAAAGAGGAGAGTTCTCCAAGGAAAACTAGCCTGGTCATTGTTGAGTCAACAGACGACCAG
  TCTCAGGTCTTTGAAAGACTGGATGGTGATGCCGCTTTTCAAAAGGGAGACGATATGCCTGACATACCCC
  CAGAGACGGTCACAGAGGAAGAATATGTTGATGAGAATGGACACACCGTGGTGAAGAAGGTTACCCGGAA
  AATCATTAGGCGGTACGTTTCCTCTGATGGCACAGAGAAGGAGGAGGTTACCATGCAGGGAATGCCTCAG
  GAGCCAGTCAACATTGAGGATGGGGACAATTATTCCAAAGTGATAAAGCGCGTGGTATTGAAGAGTGACA
  CCCAGCAGTCAGAGGTGACTTTGTCTGAACCCAGCGTTTTGTCCAGTACCTCACAGTTTCAGGCCGAGCC
  AGTAGAAGGCCGTAGAGTCAGCAAAGTTGTTAAAACAACCATGGTACACGGAGAACGGATGGAGAAGAGT
  CTTGGGGACTCTAGCTTAGCCACTGACCTTCCTTCAGCCAAAGATGACTTTGAAGAGGACAACAATGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226071 representing NM_001034168
  Red=Cloning site Green=Tags(s)
  
  MVDRQSQGTTPDTTPARTPTEEGTPTSEQNPFLFQEGKLFEMTRSGAIDMTKRPYADESLHFFQIGQESN
  EEAISEDLKEGATGAEPPQTETTSESLELSEPKEAMDDEGELLPDDVSEEIEDLPASDANIDSQVIISAS
  TETPTKEAVSTAVEEPPTTQRSDSLSTVKQTPRPAVPGPVGQLDFSPVTRSVYSGQDDESPESSPEEQKS
  VIEIPTAPVDNVPSAESKPQIPIRTLPTLVPAPPSAEDESAFSDDFPSSLDEDSKEGGAKPKSKIPVKAP
  TQRTEWQPSPTDIPLQKTAVPQGQETLSRAPDGRSKSESDASSLDAKTKCPVKARSYIETETESRERAEG
  FESESEDGATKPKLFASRLPVKSRSTSSSGRPGTSPTRESREHFFDLYRNSIEFFEEISDEASKLVDRLT
  QSEREQEPPSDDESSSALEVSVIESLPPVDIEHSAPEDIFDTRPIWDESIETMIERIPDENGHDRAEDPQ
  DEQERMEERLAYIADHLGFSWTELARELDFTEEQIHQIRIENPNSLQDQSHALLKYWLERDGKHATDTIL
  IECLTKINRMDIVHLLETNTEPLQERMGRSYAEIEQTITLDHSEGFSVLPDELCAAKEKKEQEASKESES
  SDHPPMVSEEDISVGYSTFQDCLPKTEGDSPAAALSPQMHQEPVQQDFSGKTQDQQEYYVTTPGAEVEDP
  QKATAVPDSLCKTPEDISTPPEGTKPCLQTPVTSERGSPIVQEPEEASEPKEESSPRKTSLVIVESTDDQ
  SQVFERLDGDAAFQKGDDMPDIPPETVTEEEYVDENGHTVVKKVTRKIIRRYVSSDGTEKEEVTMQGMPQ
  EPVNIEDGDNYSKVIKRVVLKSDTQQSEVTLSEPSVLSSTSQFQAEPVEGRRVSKVVKTTMVHGERMEKS
  LGDSSLATDLPSAKDDFEEDNNE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034168
• ORF Size: 2799 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034168.1, NP_001029340.1
• RefSeq Size: 5487 bp
• RefSeq ORF: 2802 bp
• Locus ID: 109676
• Cytogenetics: 3 56.07 cM
• MW: 103.7 kDa