Crhr2 (NM_009953) Mouse Tagged ORF Clone

CAT#: MR226210

Crhr2 (Myc-DDK-tagged) - Mouse corticotropin releasing hormone receptor 2 (Crhr2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Crhr2
• Synonyms: CRF-R2; Crfr2; CRFR2alpha; CRFR2beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226210 representing NM_009953
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACCCCAGGCTCTCTTCCCAGTGCACAGCTTCTCCTCTGCCTGTTTTCCCTGCTTCCAGTGCTCC
  AGGTGGCCCAACCAGGCCAGGCACCCCAGGACCAGCCCCTGTGGACACTTTTGGAGCAGTACTGCCACAG
  GACCACAACTGGGAATTTTTCAGGTCCCTACACCTACTGCAACACGACCTTGGACCAGATCGGGACCTGC
  TGGCCACAGAGCGCACCCGGAGCCCTAGTAGAGAGACCGTGCCCCGAGTACTTCAATGGCATCAAGTACA
  ACACGACCCGGAATGCCTACAGAGAGTGCCTGGAGAACGGGACCTGGGCCTCAAGGGTCAACTACTCACA
  CTGCGAACCCATTTTGGATGACAAGAGAAAGTATGACCTGCATTACCGAATCGCCCTCATTGTCAACTAC
  CTGGGTCACTGTGTTTCCGTGGTGGCCCTGGTGGCCGCTTTCCTGCTTTTCCTAGTGCTGCGGAGTATCC
  GCTGCCTGAGGAATGTGATCCACTGGAACCTCATCACCACCTTCATTCTGAGAAACATCGCGTGGTTCCT
  GCTGCAACTCATCGACCACGAAGTGCACGAGGGCAATGAGGTCTGGTGCCGCTGCATCACCACCATCTTC
  AACTATTTTGTGGTCACCAACTTCTTCTGGATGTTTGTGGAGGGCTGCTACCTGCACACGGCCATTGTCA
  TGACGTACTCCACAGAGCACCTGCGCAAGTGGCTTTTCCTCTTCATTGGATGGTGCATTCCCTGCCCTAT
  CATCATCGCCTGGGCAGTTGGCAAACTCTACTATGAGAATGAGCAGTGCTGGTTTGGCAAGGAAGCTGGT
  GATTTGGTGGACTACATCTACCAGGGCCCCGTCATGCTTGTGCTGTTGATCAATTTTGTATTTCTGTTTA
  ACATCGTCAGGATCCTGATGACGAAGTTACGAGCATCCACCACGTCCGAGACAATCCAATACAGGAAGGC
  AGTGAAGGCCACGCTGGTCCTCCTCCCCCTGTTGGGCATCACCTACATGCTCTTCTTTGTCAATCCTGGC
  GAGGACGACCTGTCCCAGATTGTGTTCATCTACTTCAACTCTTTCCTGCAGTCCTTCCAGGGTTTCTTTG
  TGTCCGTTTTCTACTGCTTCTTCAATGGAGAGGTGCGCGCGGCCCTGAGAAAGCGGTGGCACCGCTGGCA
  GGACCACCACGCCCTCCGGGTGCCTGTGGCCCGGGCCATGTCCATCCCTACGTCGCCCACCAGGATCAGC
  TTCCACAGCATCAAGCAGACAGCTGCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226210 representing NM_009953
  Red=Cloning site Green=Tags(s)
  
  MGTPGSLPSAQLLLCLFSLLPVLQVAQPGQAPQDQPLWTLLEQYCHRTTTGNFSGPYTYCNTTLDQIGTC
  WPQSAPGALVERPCPEYFNGIKYNTTRNAYRECLENGTWASRVNYSHCEPILDDKRKYDLHYRIALIVNY
  LGHCVSVVALVAAFLLFLVLRSIRCLRNVIHWNLITTFILRNIAWFLLQLIDHEVHEGNEVWCRCITTIF
  NYFVVTNFFWMFVEGCYLHTAIVMTYSTEHLRKWLFLFIGWCIPCPIIIAWAVGKLYYENEQCWFGKEAG
  DLVDYIYQGPVMLVLLINFVFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPG
  EDDLSQIVFIYFNSFLQSFQGFFVSVFYCFFNGEVRAALRKRWHRWQDHHALRVPVARAMSIPTSPTRIS
  FHSIKQTAAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009953
• ORF Size: 1290 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009953.1, NM_009953.2, NM_009953.3, NM_009953.4, NP_034083.2
• RefSeq Size: 2655
• RefSeq ORF: 1293
• Locus ID: 12922
• MW: 50.2 kDa
• Gene Summary: G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. [UniProtKB/Swiss-Prot Function]