Prkce (NM_011104) Mouse Tagged ORF Clone

CAT#: MR226230

Prkce (Myc-DDK-tagged) - Mouse protein kinase C, epsilon (Prkce)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prkce
• Synonyms: 5830406C15Rik; Pkce; PKCepsilon; PKC[e]; R75156
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226230 representing NM_011104
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGTGTTCAATGGCCTTCTTAAGATCAAAATCTGCGAGGCGGTGAGCTTGAAGCCCACAGCCTGGT
  CGCTGCGCCATGCGGTGGGACCCCGGCCACAGACGTTCCTTTTGGACCCCTACATTGCCCTTAACGTGGA
  CGACTCGCGCATCGGCCAAACAGCCACCAAGCAAAAGACCAACAGCCCGGCCTGGCACGATGAGTTCGTC
  ACCGATGTGTGCAATGGGCGCAAGATCGAGCTGGCTGTCTTTCACGACGCTCCTATCGGCTACGACGACT
  TCGTGGCCAACTGCACCATCCAGTTCGAGGAGCTGCTGCAGAATGGGAGCCGTCACTTCGAGGACTGGAT
  TGACCTGGAGCCAGAAGGAAAAGTGTACGTGATCATCGATCTCTCGGGATCATCGGGTGAAGCCCCTAAA
  GACAATGAAGAACGAGTGTTCAGGGAGCGTATGCGGCCAAGGAAGCGGCAAGGGGCTGTCAGGCGCAGGG
  TCCACCAGGTCAATGGCCACAAGTTCATGGCCACCTACTTGCGGCAACCCACCTACTGCTCCCACTGCAG
  AGATTTCATCTGGGGTGTCATAGGAAAACAGGGATATCAATGTCAAGTTTGCACTTGCGTTGTCCACAAG
  CGATGTCATGAGCTCATTATTACAAAGTGCGCTGGGCTGAAGAAACAGGAAACCCCTGACGAGGTGGGCT
  CCCAACGGTTCAGCGTCAACATGCCCCACAAGTTCGGGATCCACAACTACAAGGTCCCCACGTTCTGTGA
  CCACTGTGGGTCCCTGCTCTGGGGCCTCTTGCGGCAGGGCTTGCAGTGTAAAGTCTGCAAAATGAATGTT
  CACCGGCGATGTGAGACCAATGTGGCTCCCAACTGTGGGGTAGACGCCAGAGGAATTGCCAAAGTGCTGG
  CTGACCTTGGTGTTACTCCAGACAAAATCACCAACAGTGGCCAAAGGAGGAAAAAGCTCGCTGCTGGTGC
  TGAGTCCCCACAGCCGGCTTCTGGAAACTCCCCATCTGAAGACGACCGATCCAAGTCAGCGCCCACCTCC
  CCTTGTGACCAGGAACTAAAAGAACTTGAAAACAACATTCGGAAGGCCTTGTCATTTGACAACCGAGGAG
  AGGAGCACCGAGCGTCGTCGGCCACCGATGGCCAGCTGGCAAGCCCCGGAGAGAACGGGGAAGTCCGGCC
  AGGCCAGGCCAAGCGCTTGGGGCTGGATGAGTTCAACTTCATCAAGGTGTTGGGCAAAGGCAGCTTTGGC
  AAGGTCATGTTGGCGGAACTCAAAGGCAAAGATGAAGTCTACGCTGTGAAGGTCTTGAAGAAGGACGTTA
  TCCTACAAGACGATGATGTGGACTGCACAATGACAGAGAAGAGGATTTTGGCTCTGGCTCGGAAACACCC
  TTATCTAACCCAACTCTATTGCTGCTTCCAGACCAAGGACCGCCTCTTCTTCGTCATGGAATATGTAAAT
  GGTGGAGACCTCATGTTCCAGATTCAGCGGTCCCGAAAATTTGATGAGCCTCGTTCTCGGTTCTATGCCG
  CAGAGGTCACATCAGCCCTCATGTTTCTCCACCAGCACGGAGTGATCTACAGGGATTTGAAACTGGACAA
  CATCCTTCTAGATGCAGAAGGCCACTGCAAGCTGGCTGACTTTGGGATGTGCAAGGAAGGGATTATGAAT
  GGTGTGACAACTACCACCTTCTGTGGGACTCCTGACTACATAGCTCCAGAGATCCTACAGGAGTTGGAGT
  ACGGCCCCTCAGTGGACTGGTGGGCCCTGGGGGTGCTGATGTACGAGATGATGGCTGGGCAGCCCCCCTT
  TGAAGCTGACAACGAGGACGACTTGTTCGAATCCATCCTTCATGATGATGTTCTCTATCCTGTCTGGCTT
  AGCAAGGAAGCTGTCAGCATCCTGAAAGCTTTCATGACCAAGAACCCGCACAAGCGCCTGGGCTGTGTGG
  CAGCGCAGAACGGGGAGGACGCCATCAAGCAACATCCATTCTTCAAGGAGATTGACTGGGTACTGCTGGA
  GCAGAAGAAAATCAAGCCCCCCTTCAAGCCGAGAATTAAAACCAAAAGAGATGTCAATAACTTTGACCAA
  GACTTTACGCGGGAAGAGCCAATACTTACACTTGTGGATGAAGCAATCATTAAGCAGATCAACCAGGAAG
  AATTCAAAGGCTTCTCCTACTTTGGTGAAGACCTGATGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226230 representing NM_011104
  Red=Cloning site Green=Tags(s)
  
  MVVFNGLLKIKICEAVSLKPTAWSLRHAVGPRPQTFLLDPYIALNVDDSRIGQTATKQKTNSPAWHDEFV
  TDVCNGRKIELAVFHDAPIGYDDFVANCTIQFEELLQNGSRHFEDWIDLEPEGKVYVIIDLSGSSGEAPK
  DNEERVFRERMRPRKRQGAVRRRVHQVNGHKFMATYLRQPTYCSHCRDFIWGVIGKQGYQCQVCTCVVHK
  RCHELIITKCAGLKKQETPDEVGSQRFSVNMPHKFGIHNYKVPTFCDHCGSLLWGLLRQGLQCKVCKMNV
  HRRCETNVAPNCGVDARGIAKVLADLGVTPDKITNSGQRRKKLAAGAESPQPASGNSPSEDDRSKSAPTS
  PCDQELKELENNIRKALSFDNRGEEHRASSATDGQLASPGENGEVRPGQAKRLGLDEFNFIKVLGKGSFG
  KVMLAELKGKDEVYAVKVLKKDVILQDDDVDCTMTEKRILALARKHPYLTQLYCCFQTKDRLFFVMEYVN
  GGDLMFQIQRSRKFDEPRSRFYAAEVTSALMFLHQHGVIYRDLKLDNILLDAEGHCKLADFGMCKEGIMN
  GVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDVLYPVWL
  SKEAVSILKAFMTKNPHKRLGCVAAQNGEDAIKQHPFFKEIDWVLLEQKKIKPPFKPRIKTKRDVNNFDQ
  DFTREEPILTLVDEAIIKQINQEEFKGFSYFGEDLMP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011104
• ORF Size: 2211 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011104.1, NM_011104.2, NM_011104.3, NP_035234.1
• RefSeq Size: 6254
• RefSeq ORF: 2214
• Locus ID: 18754
• MW: 84 kDa