Dnm1 (NM_010065) Mouse Tagged ORF Clone

CAT#: MR226407

Dnm1 (Myc-DDK-tagged) - Mouse dynamin 1 (Dnm1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnm1
• Synonyms: AI838169; Dnm; Ftfl; mKIAA4093
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226407 representing NM_010065
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACCGCGGCATGGAAGACCTCATCCCGCTGGTTAACCGGTTACAGGACGCCTTCTCCGCCATCG
  GCCAGAACGCGGACCTCGACCTGCCGCAGATCGCCGTGGTAGGCGGCCAGAGCGCCGGCAAGAGCTCGGT
  GCTGGAGAATTTCGTGGGCAGGGACTTCTTGCCCCGAGGATCTGGCATCGTCACCCGGCGTCCCCTGGTC
  CTGCAGCTGGTTAATTCTACCACAGAATATGCCGAGTTCCTGCACTGCAAGGGGAAGAAATTCACCGACT
  TCGAGGAGGTGCGCCTGGAGATCGAGGCTGAGACCGATCGAGTCACCGGCACCAACAAGGGCATTTCGCC
  AGTGCCCATCAACCTGCGGGTCTACTCGCCCCATGTGCTGAACCTGACTCTAGTGGACCTGCCAGGAATG
  ACCAAGGTCCCAGTTGGGGACCAACCTCCTGATATCGAGTTCCAGATCCGGGACATGCTTATGCAGTTCG
  TCACTAAGGAGAACTGCCTTATCCTGGCTGTGTCCCCTGCCAACTCGGATTTGGCCAACTCTGATGCCCT
  CAAGATCGCTAAGGAGGTGGACCCCCAGGGTCAGCGCACCATTGGGGTCATCACCAAGTTGGACCTGATG
  GACGAGGGCACAGATGCGCGGGACGTGCTAGAGAACAAGCTGCTCCCTTTGCGCAGAGGTTACATCGGCG
  TGGTGAACCGGAGCCAGAAGGACATAGACGGCAAGAAGGACATCACAGCCGCCTTGGCTGCAGAGCGCAA
  ATTCTTCCTCTCTCACCCATCCTACCGCCACTTGGCTGACCGCATGGGCACACCCTACCTGCAGAAGGTC
  CTCAATCAGCAATTGACCAACCACATCCGGGACACACTGCCGGGACTTCGGAACAAGCTGCAGAGCCAGC
  TGCTGTCCATTGAGAAGGAGGTGGACGAGTACAAGAACTTCCGACCGGATGACCCAGCGCGCAAGACCAA
  GGCCCTGCTGCAGATGGTCCAGCAGTTTGCAGTGGACTTCGAGAAGCGCATCGAGGGTTCTGGAGACCAG
  ATTGACACTTACGAGCTGTCAGGTGGAGCCCGCATTAACCGGATCTTCCATGAACGCTTCCCCTTTGAGC
  TGGTTAAGATGGAGTTTGATGAGAAGGAACTGCGAAGGGAGATCAGCTATGCTATCAAAAATATCCATGG
  CATCAGAACGGGGCTCTTTACCCCAGACATGGCCTTTGAAACCATTGTGAAAAAGCAGGTGAAGAAGATT
  CGAGAGCCGTGTCTCAAGTGTGTGGACATGGTTATCTCGGAGCTAATCAGCACGGTTAGACAGTGCACCA
  AGAAGCTGCAGCAATACCCGCGTCTGCGGGAGGAGATGGAGCGAATTGTGACCACCCACATCCGGGAACG
  TGAGGGCCGCACCAAGGAGCAGGTCATGCTTCTCATCGACATTGAGCTGGCTTACATGAATACCAACCAC
  GAAGACTTCATAGGCTTTGCCAATGCTCAGCAGAGAAGCAACCAGATGAACAAGAAGAAGACTTCAGGGA
  ACCAGGTCATTCGAAAGGGGTGGTTGACCATCAACAACATCGGCATCATGAAGGGAGGCTCCAAGGAGTA
  CTGGTTTGTGCTGACTGCTGAGAATCTGTCCTGGTACAAGGATGATGAGGAGAAAGAGAAGAAGTACATG
  CTGTCTGTGGACAATCTGAAGCTGCGTGATGTGGAGAAGGGCTTCATGTCAAGCAAGCATATTTTTGCCC
  TCTTCAACACAGAGCAGAGGAATGTCTACAAGGATTACCGGCAGCTGGAACTGGCCTGTGAGACACAGGA
  GGAGGTGGACAGTTGGAAGGCTTCCTTCCTGAGGGCTGGCGTGTACCCTGAGCGTGTTGGGGACAAAGAG
  AAAGCCAGTGAGACCGAGGAGAACGGCTCTGACAGCTTCATGCACTCGATGGACCCTCAGCTGGAGCGCC
  AGGTGGAGACCATCCGGAACCTGGTAGACTCGTACATGGCCATTGTCAACAAGACTGTGCGGGACCTCAT
  GCCCAAGACCATCATGCACCTCATGATCAACAACACCAAGGAGTTTATCTTCTCTGAGCTGCTGGCCAAC
  CTGTACTCTTGCGGGGACCAGAACACACTGATGGAAGAATCGGCCGAGCAGGCTCAGCGGCGCGACGAGA
  TGCTGCGCATGTACCACGCACTGAAGGAGGCGCTCAGTATTATCGGCGACATCAACACGACCACCGTCAG
  CACGCCCATGCCCCCGCCCGTGGACGACTCCTGGCTGCAGGTGCAGAGCGTACCGGCCGGACGCAGATCG
  CCCACGTCCAGCCCCACGCCGCAGCGCCGAGCCCCCGCCGTGCCCCCAGCCCGGCCCGGATCGCGGGGCC
  CTGCTCCTGGGCCTCCGCCTGCTGGATCCGCCCTGGGGGGGGCGCCCCCCGTGCCCTCCAGGCCGGGGGC
  TTCCCCTGACCCCTTTGGCCCCCCTCCCCAGGTGCCCTCGCGCCCCAACCGCGCCCCGCCTGGGGTCCCC
  AGCAGGAAGGGCCCAGCCTCACCTACGAGACCTGCAGCCCCCCGACCAACGGAGGCTCCCCTCTTAGACT
  TA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226407 representing NM_010065
  Red=Cloning site Green=Tags(s)
  
  MGNRGMEDLIPLVNRLQDAFSAIGQNADLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLV
  LQLVNSTTEYAEFLHCKGKKFTDFEEVRLEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLVDLPGM
  TKVPVGDQPPDIEFQIRDMLMQFVTKENCLILAVSPANSDLANSDALKIAKEVDPQGQRTIGVITKLDLM
  DEGTDARDVLENKLLPLRRGYIGVVNRSQKDIDGKKDITAALAAERKFFLSHPSYRHLADRMGTPYLQKV
  LNQQLTNHIRDTLPGLRNKLQSQLLSIEKEVDEYKNFRPDDPARKTKALLQMVQQFAVDFEKRIEGSGDQ
  IDTYELSGGARINRIFHERFPFELVKMEFDEKELRREISYAIKNIHGIRTGLFTPDMAFETIVKKQVKKI
  REPCLKCVDMVISELISTVRQCTKKLQQYPRLREEMERIVTTHIREREGRTKEQVMLLIDIELAYMNTNH
  EDFIGFANAQQRSNQMNKKKTSGNQVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSWYKDDEEKEKKYM
  LSVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFLRAGVYPERVGDKE
  KASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMINNTKEFIFSELLAN
  LYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGDINTTTVSTPMPPPVDDSWLQVQSVPAGRRS
  PTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSALGGAPPVPSRPGASPDPFGPPPQVPSRPNRAPPGVP
  SRKGPASPTRPAAPRPTEAPLLDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010065
• ORF Size: 2592 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010065.1, NM_010065.2, NM_010065.3, NP_034195.2
• RefSeq Size: 3843
• RefSeq ORF: 2595
• Locus ID: 13429
• MW: 97.7 kDa
• Gene Summary: This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein is a GTPase which is required for membrane recycling, including vesicle endocytosis in neurons. It may also be involved in cellular fission via association with microtubules and actin filaments. Mutations in this gene have been shown to cause seizures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]