Dna2 (NM_177372) Mouse Tagged ORF Clone

CAT#: MR226448

Dna2 (Myc-DDK-tagged) - Mouse DNA replication helicase 2 homolog (yeast) (Dna2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dna2
• Synonyms: Dna2l; E130315B21Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226448 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGCTGGACGAGCTGGACCTGCTGCTGCTGGAAGAGGACGGCGGAGCGGAGGCCGTGCCGCGGG
  TGGAGCTACTTCGGAAGAAGGCGGACGCTTTGTTTCCAGAGACAGTTCTGAGCCGAGGAGTGGATAACCG
  GTACCTGGTGCTAGCTGTTGAGACCTCGCAGAATGAAAGAGGAGCCGAGGAGAAGCGCTTGCACGTCACG
  GCGTCCCAGGACCGAGAGCACGAAGTTCTGTGCATCCTGAGGAATGGCTGGAGCTCAGTACCAGTTGAGC
  CAGGGGACATCGTTCACTTAGAGGGAGACTGCACATCTGAGCCGTGGATAATAGACGATGACTTTGGATA
  TTTTATTCTGTACCCGGACATGATGATCTCCGGCACAAGCGTCGCTAGCAGCATTCGGTGTCTGAGAAGA
  GCTGTCCTGAGCGAGACCTTCAGGGGCTCTGATCCAGCCACTCGCCAGATGCTGATCGGTACAATTCTCC
  ACGAAGTCTTTCAGAAAGCGATAAGTGAGAGCTTTGCTCCAGAAAGGCTACAAGAGCTTGCTCTTCAGAC
  CCTCCGAGAAGTGAGGCATTTGAAGGAAATGTATCGCTTGAATCTGAGCCAAGATGAAATACTCTGTGAA
  GTTGAGGAGTATCTCCCTTCTTTCTCTAAGTGGGCGGAGGACTTCATGCGTAAGGGCCCTTCGTCTGAAT
  TCCCTCAAATGCAGCTCTCTCTGCCAAGCGATGGCAGTAACAGAAGTTCACCTTGTAACATTGAAGTAGT
  CAAGTCACTGGATATCGAAGAAAGCATCTGGTCCCCTAGGTTTGGACTGAAGGGCAAAATAGACGTCACA
  GTCGGGGTCAAAATACATCGAGATTGTAAAATGAAATACAAGGTAATGCCACTGGAGCTGAAGACCGGCA
  AAGAGTCCAACTCCATCGAGCACCGGAGTCAGGTGGTCCTGTACACGCTGCTAAGCCAGGAGAGGCGAGA
  GGACCCCGAGGCTGGCTGGCTTCTCTACCTCAAGACCGGGCAGATGTACCCTGTGCCCGCCAACCATCTG
  GACAAAAGAGAACTGCTGAAGCTCAGGAACTGGCTGGCCGCCTCGCTGCTGCACCGTGTGAGCCGAGCAG
  CTCCGGGGGAGGAAGCTCGGCTCTCTGCCTTGCCACAGATAATCGAGGAAGAGAAAACTTGTAAATACTG
  TTCGCAGATAGGCAACTGTGCTCTTTATAGCAGAGCAGTTGAAGAGCAGGGAGATGACGCATCCATCCCA
  GAAGCCATGCTGTCCAAAATCCAGGAAGAAACACGGCACCTGCAGCTGGCGCACTTGAAGTATTTCAGCC
  TGTGGTGTCTCATGTTAACGCTGGAGTCGCAATCTAAAGACAACCGGAAGACTCACCAGAGTATCTGGTT
  AACGCCTGCCTCTGAACTGGAGGAGAGTGGCAACTGTGTTGGAAACCTGGTCAGGACGGAGCCTGTATCA
  AGAGTTTGTGATGGACAGTATTTACATAATTTTCAGCGGAAAAATGGTCCCATGCCAGCCACCAATCTGA
  TGGCAGGTGACAGGATTATTTTAAGTGGAGAAGAGAGAAAACTATTTGCTTTGTCCAAAGGCTACGTGAA
  GAAGATGAACAAGGCAGCCGTTACCTGCTTGCTAGACAGGAACTTGTCGACACTCCCAGCAACAACGGTG
  TTTCGATTAGACCGGGAAGAAAGACATGGCGACATAAGTACTCCACTAGGAAATCTGTCTAAATTGATGG
  AAAGCACAGATCCCAGCAAAAGACTTCGAGAATTAATCATCGATTTCAGGGAACCTCAGTTCATCGCCTA
  CCTCAGCTCCGTCCTTCCACACGATGCGAAGGATACGGTGGCCAACATTCTAAAGGGTTTGAATAAACCC
  CAGAGACAAGCAATGAAGAGAGTCCTTCTTTCGAAAGACTACACGCTCATTGTTGGGATGCCAGGGACAG
  GAAAGACAACCACCATCTGTGCCCTGGTGAGGATCCTCTCTGCCTGTGGGTTCAGCGTTCTGCTGACCAG
  CTACACGCACTCCGCCGTTGACAATATTCTCCTGAAGTTAGCCAAGTTTAAAGTCGGATTTTTGCGCTTG
  GGTCAGTCTCATAAGGTCCATCCAGACATCCAGAAGTTTACAGAGGAGGAGATTTGCAGATCGAGGTCCA
  TTGCGTCTTTAGCCCATCTAGAAGAACTCTATAACAGTCACCCTATTGTTGCAACAACCTGCATGGGAAT
  AAATCACCCAATATTTTCCCGAAAAACCTTTGATTTCTGTATTGTGGATGAAGCATCTCAAATCAGCCAG
  CCCGTCTGCCTGGGCCCACTGTTCTTTTCGCGGAGGTTTGTGCTGGTGGGGGACCATCAGCAGCTGCCTC
  CCCTGGTTGTAAACCGAGAGGCAAGAGCTCTGGGCATGAGCGAGAGCTTGTTCAAGAGGCTGGAGCGGAA
  CGAGAGCGCTGTGGTGCAGCTGACCGTGCAATACCGAATGAACAGGAAGATTATGTCCCTGAGCAACAAG
  CTCACATACGCAGGGAAACTGGAGTGTGGGTCAGACCGAGTGGCCAACGCAGTGCTAGCCCTGCCCAACC
  TCAAGGACGCAAGGCTGAGCCTCCAGCTTTATGCCGACTACTCTGACAGCCCCTGGCTGGCTGGAGTGTT
  AGAGCCAGACAATCCCGTGTGTTTCCTTAACACAGACAAGGTCCCAGCTCCAGAGCAAGTTGAAAATGGT
  GGTGTTAGCAATGTCACAGAAGCCAGACTCATTGTCTTCCTAACCTCAACTTTTATAAAGGCTGCAGCCC
  CTCAGACATTGGCGTCATCGCCCCGTACCGACAGCAGCTGCGGATCATCAGCGACTTACTGGCCCGGTCT
  TCTGTTGGGATGGTTGAGGTTAACACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226448 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPLDELDLLLLEEDGGAEAVPRVELLRKKADALFPETVLSRGVDNRYLVLAVETSQNERGAEEKRLHVT
  ASQDREHEVLCILRNGWSSVPVEPGDIVHLEGDCTSEPWIIDDDFGYFILYPDMMISGTSVASSIRCLRR
  AVLSETFRGSDPATRQMLIGTILHEVFQKAISESFAPERLQELALQTLREVRHLKEMYRLNLSQDEILCE
  VEEYLPSFSKWAEDFMRKGPSSEFPQMQLSLPSDGSNRSSPCNIEVVKSLDIEESIWSPRFGLKGKIDVT
  VGVKIHRDCKMKYKVMPLELKTGKESNSIEHRSQVVLYTLLSQERREDPEAGWLLYLKTGQMYPVPANHL
  DKRELLKLRNWLAASLLHRVSRAAPGEEARLSALPQIIEEEKTCKYCSQIGNCALYSRAVEEQGDDASIP
  EAMLSKIQEETRHLQLAHLKYFSLWCLMLTLESQSKDNRKTHQSIWLTPASELEESGNCVGNLVRTEPVS
  RVCDGQYLHNFQRKNGPMPATNLMAGDRIILSGEERKLFALSKGYVKKMNKAAVTCLLDRNLSTLPATTV
  FRLDREERHGDISTPLGNLSKLMESTDPSKRLRELIIDFREPQFIAYLSSVLPHDAKDTVANILKGLNKP
  QRQAMKRVLLSKDYTLIVGMPGTGKTTTICALVRILSACGFSVLLTSYTHSAVDNILLKLAKFKVGFLRL
  GQSHKVHPDIQKFTEEEICRSRSIASLAHLEELYNSHPIVATTCMGINHPIFSRKTFDFCIVDEASQISQ
  PVCLGPLFFSRRFVLVGDHQQLPPLVVNREARALGMSESLFKRLERNESAVVQLTVQYRMNRKIMSLSNK
  LTYAGKLECGSDRVANAVLALPNLKDARLSLQLYADYSDSPWLAGVLEPDNPVCFLNTDKVPAPEQVENG
  GVSNVTEARLIVFLTSTFIKAAAPQTLASSPRTDSSCGSSATYWPGLLLGWLRLTQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177372
• ORF Size: 2901 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_177372.1, NM_177372.2, NM_177372.3, NP_796346.2
• RefSeq Size: 4122
• RefSeq ORF: 3189
• Locus ID: 327762
• MW: 108.5 kDa