Ddx4 (NM_001145885) Mouse Tagged ORF Clone

CAT#: MR226455

Ddx4 (Myc-DDK-tagged) - Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (Ddx4), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ddx4
• Synonyms: AV206478; Mvh; VASA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226455 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGATGAAGATTGGGAGGCAGAAATACTCAAACCTCACGTGTCTTCCTACGTTCCTGTATTTGAGA
  AGGATAAATATTCTTCTGGAGCAAATGGAGACACTTTTAACAGGACTTCAGCTTCATCAGAAATGGAAGA
  TGGGCCTTCTGGAAGAGATGATTTCATGAGAAGTGGGTTTCCTTCTGGAAGAAGTTTAGGAAGCAGAGAT
  ATTGGCGAGTCTAGTAAAAAAGAGAACACATCTACAACTGGTGGCTTTGGAAGAGGAAAGGGCTTTGGAA
  ACAGAGGTTTTTTAAATAACAAGTTTGAAGAAGGTGATAGCTCTGGTTTCTGGAAAGAGTCTAATAATGA
  CTGTGAAGATAATCAGACTCGAAGCAGAGGGTTTTCCAAGCGAGGTGGCTGCCAAGATGGAAACGATTCA
  GAAGCATCAGGCCCGTTCAGAAGAGGGGGAAGAGGCAGTTTCCGAGGCTGCCGTGGAGGATTTGGTCTAG
  GAAGACCAAATAGTGAATCTGACCAAGATCAGGGGACACAGCGTGGTGGTGGCCTTTTTGGTTCTAGGAA
  ACCAGCAGCAAGTGATTCAGGCAATGGTGACACTTATCAAAGCAGAAGTGGAAGTGGTCGAGGTGGTTAC
  AAAGGTTTAAATGAAGAAGTAGTAACAGGTTCTGGAAAGAATTCTTGGAAGTCAGAAACTGAAGGAGGTG
  AAAGCAGTGATAGTCAAGGTCCAAAAGTGACATATATACCCCCTCCTCCACCAGAGGATGAGGACTCCAT
  CTTTGCACATTATCAGACAGGCATAAACTTTGATAAATATGACACCATACTTGTTGAAGTATCTGGACAT
  GATGCACCACCGGCAATTTTGACTTTTGAAGAAGCTAATCTCTGTCAGACACTGAATAACAACATTGCTA
  AAGCTGGCTATACTAAGCTTACTCCTGTGCAGAAGTACAGCATTCCCATTGTATTAGCAGGACGAGATTT
  GATGGCTTGTGCTCAAACAGGGTCTGGGAAGACTGCAGCTTTTCTCTTGCCTATTTTGGCTCATATGATG
  CGGGATGGAATAACTGCCAGTCGCTTTAAAGAACTGCAGGAACCAGAGTGTATTATTGTAGCACCAACTC
  GAGAACTGATCAACCAAATTTACTTGGAAGCCAGAAAATTTTCTTTTGGGACTTGTGTAAGAGCTGTGGT
  CATATATGGAGGAACCCAGTTTGGTCATTCAGTTCGACAGATAGTACAAGGGTGTAACATATTGTGTGCT
  ACTCCAGGGAGGCTGATGGACATCATAGGCAAAGAAAAGATTGGCCTCAAACAAGTCAAGTACTTAGTTT
  TGGATGAAGCTGATCGAATGTTGGATATGGGTTTTGGACCAGAGATGAAGAAACTAATTTCTTGTCCAGG
  AATGCCATCAAAGGAACAACGCCAAACCCTTTTATTCAGTGCTACTTTTCCAGAAGAAATCCAGAGGTTG
  GCTGGGGACTTTCTAAAGTCCAGTTACTTGTTTGTCGCTGTTGGGCAAGTGGGAGGAGCTTGCAGAGATG
  TTCAGCAGACGATCCTTCAAGTTGGCCAGTATTCAAAAAGAGAAAAGCTTGTTGAGATTCTACGAAACAT
  AGGTGATGAAAGAACTATGGTCTTTGTTGAAACCAAGAAAAAAGCCGATTTCATTGCAACTTTTCTTTGT
  CAAGAAAAAATATCAACTACAAGTATTCATGGTGATCGGGAACAGAGGGAGAGAGAGCAAGCTCTTGGAG
  ATTTTCGCTGTGGAAAGTGCCCAGTTCTTGTTGCTACTTCAGTGGCTGCCAGAGGGCTTGATATTGAAAA
  TGTTCAACATGTTATCAATTTTGACCTTCCTTCTACCATTGATGAGTATGTTCATCGAATTGGACGCACT
  GGCCGCTGTGGAAATACTGGCAGAGCGATTTCTTTTTTTGATACTGACTCTGATAATCATTTAGCACAGC
  CTCTAGTTAAAGTACTGTCAGACGCTCAACAGGATGTCCCCGCATGGCTAGAAGAGATTGCCTTCAGTAC
  CTATGTGCCTCCCAGCTTCAGTAGCAGCACAAGAGGGGGGGCTGTGTTTGCATCTGTTGACACGAGGAAG
  AATTACCAGGGCAAGCACACGTTGAATACAGCGGGGATTTCTTCTTCACAAGCTCCCAATCCAGTTGATG
  ACGAGTCATGGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226455 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDEDWEAEILKPHVSSYVPVFEKDKYSSGANGDTFNRTSASSEMEDGPSGRDDFMRSGFPSGRSLGSRD
  IGESSKKENTSTTGGFGRGKGFGNRGFLNNKFEEGDSSGFWKESNNDCEDNQTRSRGFSKRGGCQDGNDS
  EASGPFRRGGRGSFRGCRGGFGLGRPNSESDQDQGTQRGGGLFGSRKPAASDSGNGDTYQSRSGSGRGGY
  KGLNEEVVTGSGKNSWKSETEGGESSDSQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGH
  DAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIVLAGRDLMACAQTGSGKTAAFLLPILAHMM
  RDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQFGHSVRQIVQGCNILCA
  TPGRLMDIIGKEKIGLKQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLLFSATFPEEIQRL
  AGDFLKSSYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLC
  QEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRT
  GRCGNTGRAISFFDTDSDNHLAQPLVKVLSDAQQDVPAWLEEIAFSTYVPPSFSSSTRGGAVFASVDTRK
  NYQGKHTLNTAGISSSQAPNPVDDESWD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145885
• ORF Size: 2187 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145885.1, NP_001139357.1
• RefSeq Size: 2850
• RefSeq ORF: 2187
• Locus ID: 13206
• MW: 79.3 kDa