Blm (NM_001042527) Mouse Tagged ORF Clone

CAT#: MR226518

Blm (Myc-DDK-tagged) - Mouse Bloom syndrome, RecQ helicase-like (Blm), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Blm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226518 representing NM_001042527
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGTTCCTCTGAACAATCTACAAGAACAACTACAGCGACACTCAGCCAGAAAACTTAATAATC
  AACCCAGCCTTTCAAAACCAAAATCTTTAGGTTTTACTTTTAAAAAGAAAACATCAGAGGGTGATGTGTC
  TGTCACTAGTGTGTCCGTAGTAAAAACACCTGCGTTAAGTGATAAAGATGTGAACGTGTCTGAGGCCTTT
  TCATTCACTGAGTCTCCACTCCACAAACCAAAGCAGCAGGCAAAGATTGAAGGCTTCTTTAAACATTTCC
  CTGGAAGGCAGCAAAGCAAGGGGACCTGCTCTGAGCCGTCACTGCCGGCCACGGTACAGACTGCTCAGGA
  CACTTTGTGCACTACCCCCAAAACCCCCACTGCGAAGAAACTGCCCGTGGCTGTTTTCAAGAAATTAGAA
  TTTAGTTCTTCTGCAGACTCCCTCAGTGACTGGGCTGATATGGATGACTTTGATATGTCAGCATCAGATG
  CGTTTGCTTCACTGGCTAAAAATCCTGCCACAAGAGTAAGCACCGCTCAGAAAATGAAAAAGACTAAGAG
  AAACTTCTTTAAACCACCACCTCGTAAAGCCAATGCAGTAAAGACTGACTTGACTCCTCCCTCCCCCGAA
  TGCCTGCAAGTGGATTTAACGAAGGAATCGGAGGAGGAGGAGGAGGAGGAGGAGGAGGCGGAGGGGGCGG
  ACTGCCTGAGCAGGGATGTGATCTGCATTGACAATGATTCTGCTTCTGAAGAGCTCACGGAGAAAGACAC
  GCAGGAAAGCCAGTCTTTGAAAGCTCACTTGGGAGCTGAAAGAGGTGACAGTGAAAAGAAGAGCCATGAA
  GACGAAGCTGTGTTCCATTCAGTTCAGAACACTGAATACTTTGAACACAATGACAATGATTATGATATAG
  ATTTTGTTCCACCTTCTCCAGAAGAAATCATCTCCACTGCTTCTTCCTCGTTGAAATGTTCCAGTATGTT
  AAAGGATCTTGATGACTCTGACAAAGAAAAGGGCATTCTTAGCACCTCAGAAGAGCTTCTGTCAAAACCA
  GAGGAAATGACCACACACAAGTCTGATGCAGGAACCAGTAAAGACTGTGATGCCCAGCAGATACGCATAC
  AGCAGCAGCTTATTCATGTGATGGAGCACATCTGTAAGTTAGTTGATACTGTTCCTACTGATGAACTGGA
  AGCTTTGAATTGTGGGACCGAATTGCTTCAACAACGAAACATAAGGAGGAAGCTCCTAGCTGAAGCAGGT
  TTTAATGGAAATGACGTCAGACTTCTGGGTTCTCTGTGGAGGCACAGGCCTGATTCACTTGATAACACAG
  TGCAGGGCGACTCCTGCCCTGTGGGGCATCCTAATAAAGAGTTAAATTCTCCATACCTTCTCTCACATTC
  CCCTTCCACTGAGGAATGTTTACCCACCACCACTCCAGGAAAGACAGGATTCTCAGCCACCCCGAAGAAT
  CTCTTTGAAAGGCCGTTATTGAATTCCCATTTACAGAAGTCCTTTGTAAGTAGCAACTGGGCTGAAACAC
  CAAGGATGGAAAACAGGAACGAAAGCACTGACTTCCCAGGGAGTGTTCTCACCAGCACCACTGTGAAAGC
  TCAGAGTAAACAAGCTGCTTCAGGATGGAACGTAGAGAGACACGGCCAGGCTTCCTATGATATCGATAAC
  TTTAATATTGATGACTTTGATGATGATGATGATGATGATGACTGGGAAAACATAATGCACAATTTTCCAG
  CCAGCAAATCTTCCACAGCCACCTACCCACCCATCAAGGAAGGTGGGCCAGTTAAATCTCTCTCAGAAAG
  GATTTCTTCAGCCAAGGCAAAGTTTCTTCCAGTGGTATCAACCGCTCAAAATACAAACCTCTCAGAGTCA
  ATTCAGAATTGCTCTGATAAGCTGGCCCAAAATTTATCATCAAAAAATCCAAAACATGAACATTTTCAAA
  GTCTTAATTTTCCTCATACAAAAGAAATGATGAAGATTTTCCATAAGAAATTTGGCTTGCATAATTTTAG
  AACTAATCAGCTAGAGGCGATCAATGCTGCGCTGCTTGGTGAAGACTGCTTTATCCTAATGCCCACTGGA
  GGAGGTAAAAGTTTGTGCTACCAGCTCCCTGCCTGTGTTTCTCCTGGGGTCACAATTGTCATTTCTCCCT
  TGAGATCACTAATAGTAGATCAAGTCCAAAAGCTGACTTCCTTTGATATTCCAGCTACATATCTGACAGG
  GGATAAGACTGACTCAGAAGCTGCAAATATTTACCTCCAATTATCCAAAAAAGACCCAATCATCAAGCTT
  TTGTATGTTACTCCAGAGAAGGTCTGTGCAAGTAACAGGCTGATTTCTACTCTGGAGAATCTGTATGAGC
  GGAAGCTCTTGGCACGTTTTGTCATTGATGAAGCGCATTGTGTGAGTCAGTGGGGTCATGATTTTCGTCA
  AGATTACAAAAGGATGAATATGCTTCGCCAGAAGTTTCCTTCTGTTCCAGTGATGGCCCTCACGGCCACA
  GCGAACCCCAGGGTCCAGAAGGACATCCTCACTCAGCTGAAGATCCTCAGACCTCAGGTGTTTAGCATGA
  GCTTTAACAGACACAATCTGAAGTACTATGTATTACCCAAGAAGCCCAAAAAAGTAGCATTTGATTGCCT
  AGAGTGGATCAGAAAGCATCACCCTTATGACTCGGGGATAATTTACTGCCTCTCCAGGAGGGAATGTGAC
  ACAATGGCTGACACTTTACAGAGAGAAGGCCTGGCTGCCCTGGCTTACCATGCGGGCCTCAGTGACTCTG
  CCAGAGATGAGGTGCAGCACAAGTGGATCAACCAGGACAACTGCCAGGTTATCTGTGCGACAATTGCGTT
  TGGAATGGGAATTGACAAACCTGACGTGCGATTTGTGATTCATGCATCTCTTCCTAAATCTATGGAGGGT
  TATTATCAAGAATCCGGCCGAGCTGGAAGAGATGGGGAAATATCTCACTGCGTGCTTTTCTATACATATC
  ATGATGTGACCAGACTGAAGAGACTTATAATGATGGAAAAAGATGGAAACTATCATACGAAGGAAACTCA
  CGTCAATAATCTATATAGCATGGTACATTACTGTGAAAACATAACGGAATGCAGAAGAATACAGCTTTTA
  GCTTACTTTGGTGAAAAAGGATTCAACCCTGATTTTTGTAAGAAATACCCAGATGTTTCTTGTGACAATT
  GCTGTAAAACAAAGGATTATAAAACAAAAGATGTGACTGATGACGTGAAAAATATTATAAGATTTGTTCA
  AGAACACAGTTCATCACCAGGAACAAGAAATATAGGACCTGCTGGAAGATTTACTCTGAACATGCTGGTC
  GACATTTTCTTGGGGAGCAAGAGTGCAAAAGTTAAGTCTGGAATATTTGGAAAGGGGACTACATATTCAC
  GACATAATGCCGAAAGACTTTTTAAAAAGCTGATTCTAGACAAAATCCTGGATGAAGACTTATATATCAA
  TGCCAATGACCAACCAATTGCCTATGTGATGCTAGGAACAAAAGCCCACAGTGTGCTGAGTGGCCACTTG
  AAGGTGGACTTCATGGAAACGGAAAATTCCAGCAGTATTAAAAAACAAAAAGCTTTAGTGGCCAAAGTAT
  CCCAGAGAGAAGAGGTAGTTAAGAAATGTCTTGGAGAACTTACAGAGGTCTGCAAATTGCTGGGGAAAGT
  CTTTGGTGTCCATTACTTCAATATTTTTAATACAGCCACACTCAAAAAGCTTGCAGAATCTTTATCTTCT
  GATCCTGAGGTTTTGCTTCAGATTGATGGTGTTACCGAAGACAAGCTGGAAAAATATGGTGCAGAAGTGA
  TTCCAGTATTACAGAAGTACTCAGAATGGACAGTGCCAGCTGAGGATGGTTCCCCAGGCGCCAGAGGCGC
  CCCAGAGGACACTGAGGAGGAGGAGGAGGAAGCGCCTGTATCTTCTCACTACTTTGCAAATCAAACTAGA
  AATGAAAGAAAGAGAAAGAAAATGTCAGCCACCCATAAGCCCAAGAGGAGAAGAACTAGTTACGGTGGCT
  TCAGAGCAAAGGGGGGCTCTACTACATGCAGAAAAACGACTTCTAAAAGTAAATTCTATGGTGTAACTGG
  ATCCCGCTCGGCCTCATGTGCTTCTCAGGCAACATCATCAGCCAGTAGAAAACTGGGGATTATGGCTCCT
  CCAAAGCCTGTAAATAGAACGTTCCTCAGGCCTTCATATGCCTTCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226518 representing NM_001042527
  Red=Cloning site Green=Tags(s)
  
  MAAVPLNNLQEQLQRHSARKLNNQPSLSKPKSLGFTFKKKTSEGDVSVTSVSVVKTPALSDKDVNVSEAF
  SFTESPLHKPKQQAKIEGFFKHFPGRQQSKGTCSEPSLPATVQTAQDTLCTTPKTPTAKKLPVAVFKKLE
  FSSSADSLSDWADMDDFDMSASDAFASLAKNPATRVSTAQKMKKTKRNFFKPPPRKANAVKTDLTPPSPE
  CLQVDLTKESEEEEEEEEEAEGADCLSRDVICIDNDSASEELTEKDTQESQSLKAHLGAERGDSEKKSHE
  DEAVFHSVQNTEYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKGILSTSEELLSKP
  EEMTTHKSDAGTSKDCDAQQIRIQQQLIHVMEHICKLVDTVPTDELEALNCGTELLQQRNIRRKLLAEAG
  FNGNDVRLLGSLWRHRPDSLDNTVQGDSCPVGHPNKELNSPYLLSHSPSTEECLPTTTPGKTGFSATPKN
  LFERPLLNSHLQKSFVSSNWAETPRMENRNESTDFPGSVLTSTTVKAQSKQAASGWNVERHGQASYDIDN
  FNIDDFDDDDDDDDWENIMHNFPASKSSTATYPPIKEGGPVKSLSERISSAKAKFLPVVSTAQNTNLSES
  IQNCSDKLAQNLSSKNPKHEHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTG
  GGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKL
  LYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTAT
  ANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECD
  TMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVIHASLPKSMEG
  YYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTKETHVNNLYSMVHYCENITECRRIQLL
  AYFGEKGFNPDFCKKYPDVSCDNCCKTKDYKTKDVTDDVKNIIRFVQEHSSSPGTRNIGPAGRFTLNMLV
  DIFLGSKSAKVKSGIFGKGTTYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLSGHL
  KVDFMETENSSSIKKQKALVAKVSQREEVVKKCLGELTEVCKLLGKVFGVHYFNIFNTATLKKLAESLSS
  DPEVLLQIDGVTEDKLEKYGAEVIPVLQKYSEWTVPAEDGSPGARGAPEDTEEEEEEAPVSSHYFANQTR
  NERKRKKMSATHKPKRRRTSYGGFRAKGGSTTCRKTTSKSKFYGVTGSRSASCASQATSSASRKLGIMAP
  PKPVNRTFLRPSYAFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042527
• ORF Size: 4248 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001042527.1, NM_001042527.2, NP_001035992.1
• RefSeq Size: 4598
• RefSeq ORF: 4251
• Locus ID: 12144
• MW: 158.8 kDa