Pld2 (NM_008876) Mouse Tagged ORF Clone

CAT#: MR226603

Pld2 (Myc-DDK-tagged) - Mouse phospholipase D2 (Pld2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pld2
• Synonyms: PLD1C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226603 representing NM_008876
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGTAACCCAGAAGAACCTCTTTCCCTATGGGGACTATCTGAACTCCAGCCAGTTGCACATGGAGC
  CAGATGAGGTTGACACTCTGAGGGAAGGAGAGGATCCAGCTGATCGAATGCATCCCTATCTGGCCATCTA
  TGACCTTCAGCCTCTGAAAGCACACCCCTTGGTGTTCGCCCCTGGGGTCCCTGTTATAGCCCAGGTGGTG
  GGCACCGAAAGATACACCAGCGGATCCAAGGTGGGAACCTGTACTCTATATTCTGTTCGCTTGACGCATG
  GTGACTTTACCTGGACAACCAAGAAGAAGTTCCGACACTTTCAGGAGCTGCATCGGGACCTCCAGAGACA
  CAAAGTCTTGATGAGTCTGCTCCCTTTGGCTCGCTTTGCTGTGACCCATTCTCCAGCCCGAGAGGCAGCC
  GCCGAGGATATACCCTCCCTACCCCGAGGAGGTTCTGAGGGCTCTGCCAGACACACAGCCAGCAAACAGA
  AATACTTGGAAAATTACCTCAACCGCCTCCTGACCATGTCTTTCTATCGCAATTACCACGCCATGACAGA
  ATTTCTGGAAGTCAGTCAACTTTCCTTTATCCCAGACCTTGGCTCCAAAGGACTGGAAGGGGTGATCCGG
  AAGCGCTCGGGCGGGCATCGAGTTCCCGGCTTCACCTTCTGTGGCCGAGACCAAGTTTGTTATCGATGGT
  CCAAGAGGTGGCTGGTGGTGAAGGACTCCTTCCTGCTGTACATGCGCCCGGAGACCGGCGCCATCTCATT
  TGTTCAGCTTTTTGACCCTGGCTTTGAGGTCCAGGTCGGAAAAAGGAGCACAGAGACGCGGTATGGGGTG
  AGGATCGACACCTCCCACAGGTCCCTGATTCTCAAATGCAGCAGCTACCGGCAGGCACGGTGGTGGGGCC
  AGGAGATCACGGAGCTGGCACAGGGTTCGGGCAGAGATTTTCTACAGCTACATCAGCATGACAGCTATGC
  CCCACCCCGGCCCGGCACCCTGGCCCGGTGGTTTGTGAATGGGGCAGGTTACTTTGCTGCTGTGGCAGAT
  GCCATCCTGCGAGCTCAAGAGGAGATTTTCATCACAGACTGGTGGTTGAGTCCTGAAATTTACCTGAAGC
  GTCCAGCCCATTCCGACGACTGGAGACTGGACATTATGCTCAAGAGGAAGGCGGAAGAAGGTGTCCGAGT
  TTCCATACTGCTGTTTAAGGAAGTGGAGCTGGCCTTGGGCATCAACAGTGGCTACAGCAAGAGGACGCTG
  ATGCTGCTGCATCCCAACATAAAGGTGATGCGACACCCAGACCTTGTGACACTGTGGGCTCATCACGAGA
  AGCTCCTGGTGGTAGACCAAGTGGTGGCATTCTTGGGCGGGCTGGACCTGGCCTTCGGCCGCTGGGATGA
  CGTGCAATACCGACTGACTGACCTGGGTGACCCCTCTGAACCTGTACATTTACAGACTCCCACACTAGGT
  TCAGACCCTGCAGCCACTCCAGACCTCTCGCATAACCAATTCTTCTGGCTGGGAAAGGACTACAGCAACC
  TCATCACCAAGGACTGGGTGCAGCTGGACCGGCCTTTTGAAGATTTCATCGACAGGGAGACCACACCCAG
  GATGCCATGGAGGGATGTTGGAGTGGTTGTACACGGAGTAGCTGCCAGGGACCTTGCCCGGCACTTCATC
  CAGCGCTGGAATTTCACCAAGACCACCAAGGCCAGGTATAAGACACCTTTGTACCCCTACCTGCTGCCCA
  AGTCCACCAGCACTGCAAACAATCTCCCCTTCATGATCCCAGGCGGGCAGTGTGCCACTGTGCAGGTCTT
  GAGGTCTGTGGATCGATGGTCAGCAGGGACATTGGAGAACTCCATCCTCAATGCCTACCTACATACCATT
  CGAGAGAGCCAGCACTTTCTCTACATTGAGAATCAGTTCTTCATTAGCTGCTCAGATGGGCGAACAGTTC
  TGAACAAGGTGGGCGATGAGATTGTGGACAGAATCCTGAAGGCTCACGAACAGGGGCAGTGTTTCCGAGT
  CTACTTGCTTCTGCCTTTGCTCCCTGGCTTTGAGGGGGACATCTCCACAGGGGGTGGTAACTCCATCCAG
  GCTATTCTGCACTTCACCTACAGGACCCTGTGTCGTGGGGAACATTCAATCCTACATCGTCTCAAAGCAG
  CCATGGGGACTGCGTGGCGAGATTACATGTCCATCTGTGGGCTTCGCACCCATGGAGAGCTGGGCGGGCA
  CCCAATCTCTGAGCTCATCTATATCCACAGCAAGATGCTCATTGCGGATGACAGAACAGTCATCATTGGT
  TCTGCGAACATCAATGACAGGAGCTTGCTGGGGAAGCGTGACAGTGAGCTAGCCATCCTGATCAAGGACA
  CAGAAATGGAACCATCCCTCATGGATGGGGTGGAGTACCAGGCAGGCAGATTTGCCTTGAGTTTGCGGAA
  GCACTGTTTCAGTGTGATTCTTGGGGCAAATACCTGGCCAGACCTGGATCTCCGAGACCCTGTCTGTGAT
  GACTTCTTCCAGCTGTGGCAAGAAACAGCGGAGAACAATGCCACCATCTATGAGCAGATCTTCCGCTGCC
  TGCCGTCCAATGCTACCCGTTCCCTGCGGGCTCTCCGGGAGTATGTGGCTGTGGAGTCCTTGGCTACAGT
  CAGCCCTTCTTTGGCTCAGTCTGAGCTTGCCCACATCCAGGGCCACCTAGTTCACTTCCCCCTCAAGTTT
  CTGGAGGACGAGTCCTTGTTGCCCCCACTGGGGAGTAAAGAAGGGATGATACCTTTAGAAGTGTGGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226603 representing NM_008876
  Red=Cloning site Green=Tags(s)
  
  MTVTQKNLFPYGDYLNSSQLHMEPDEVDTLREGEDPADRMHPYLAIYDLQPLKAHPLVFAPGVPVIAQVV
  GTERYTSGSKVGTCTLYSVRLTHGDFTWTTKKKFRHFQELHRDLQRHKVLMSLLPLARFAVTHSPAREAA
  AEDIPSLPRGGSEGSARHTASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGSKGLEGVIR
  KRSGGHRVPGFTFCGRDQVCYRWSKRWLVVKDSFLLYMRPETGAISFVQLFDPGFEVQVGKRSTETRYGV
  RIDTSHRSLILKCSSYRQARWWGQEITELAQGSGRDFLQLHQHDSYAPPRPGTLARWFVNGAGYFAAVAD
  AILRAQEEIFITDWWLSPEIYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRTL
  MLLHPNIKVMRHPDLVTLWAHHEKLLVVDQVVAFLGGLDLAFGRWDDVQYRLTDLGDPSEPVHLQTPTLG
  SDPAATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGVAARDLARHFI
  QRWNFTKTTKARYKTPLYPYLLPKSTSTANNLPFMIPGGQCATVQVLRSVDRWSAGTLENSILNAYLHTI
  RESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHEQGQCFRVYLLLPLLPGFEGDISTGGGNSIQ
  AILHFTYRTLCRGEHSILHRLKAAMGTAWRDYMSICGLRTHGELGGHPISELIYIHSKMLIADDRTVIIG
  SANINDRSLLGKRDSELAILIKDTEMEPSLMDGVEYQAGRFALSLRKHCFSVILGANTWPDLDLRDPVCD
  DFFQLWQETAENNATIYEQIFRCLPSNATRSLRALREYVAVESLATVSPSLAQSELAHIQGHLVHFPLKF
  LEDESLLPPLGSKEGMIPLEVWT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008876
• ORF Size: 2799 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008876.1, NM_008876.2, NM_008876.3, NP_032902.1
• RefSeq Size: 3801
• RefSeq ORF: 2802
• Locus ID: 18806
• MW: 106.6 kDa
• Gene Summary: This gene is a member of the phospholipase D (PLD) superfamily. The encoded protein catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. Phosphatidic acid is an essential intracellular lipid second messenger for many signaling pathways and has been implicated in a variety of physiological processes including cytoskeletal organization and cell proliferation. A similar gene in human may also function as a guanine nucleotide exchange factor (GEF) for the small GTPase Rac2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]