Ehmt1 (NM_001109687) Mouse Tagged ORF Clone

CAT#: MR226605

Ehmt1 (Myc-DDK-tagged) - Mouse euchromatic histone methyltransferase 1 (Ehmt1), transcript variant 4

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ehmt1
• Synonyms: 9230102N17Rik; D330003E03; Eu-HMTase1; GLP; GLP1; KMT1D; mKIAA1876
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226605 representing NM_001109687
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCGCTGATGCTGAGCAGGCAGTTCTGGCCAAGCAAGAGACCAAGCAGGATTGCTGCATGAAAA
  CTGAGCTGCTAAGGGAAGATACACCTATGGCTGCTGATGAAGGTTCCACAGAGAAACAAGAAGGAGAGAC
  TCCCATGGCTGCAGATGGAGAAACAAATGGGTCTTGTGAAAAGAGTGGGGATCCCAGCCATCTAAATGCA
  CCCAAACACACTCAGGAGAACACAAGAGCTAGCCCACAGGAAGGCACCAACAGAGTGTCTCGGGTGGCAG
  AAAATGGGGTTTCAGAAAGAGACACAGAAGTGGGGAAGCAAAACCATGTCACAGCTGACGACTTCATGCA
  GACATCTGTCATTGGCAGCAATGGATATTTCTTAAATAAACCAGCCCTGCAGGGGCAGCCGTTGAGGACT
  CCCAACATTCTAACCTCCTCGCTTCCTGGTCATGCTGCAAAAACTCTTCCTGGAGGAGCCAGTAAATGCA
  GGACTCTGAGTGCACTTCCTCAGACACCAACCACAGCACCCACTGTGCCTGGGGAAGGGAGTGCAGACAC
  AGAGGACAGAAAGCCTACAGCCTCGGGCACTGATGTCAGGGTTCACAGGGCACGCAAGACCATGCCGAAG
  TCCATCTTGGGCCTGCATGCAGCCAGCAAAGACCATAGAGAAGTTCAAGACCATAAGGAACCAAAAGAGG
  ATATCAACAGAAACATTTCTGAATGTGGACGACAGCAGCTTTTACCAACCTTCCCAGCCCTCCACCAGTC
  GCTACCTCAGAATCAGTGCTACATGGCCACCACAAAGTCCCAGACAGCTTGCTTGCCTTTTGTTTTAGCA
  GCTGCAGTATCTCGGAAGAAAAAACGAAGAATGGGAACCTATAGTTTAGTTCCCAAGAAAAAGACCAAAG
  TATTAAAACAGAGGACGGTGATTGAGATGTTTAAAAGCATAACCCATTCCACTGTGGGCGCCAAGGGCGA
  GAAAGCCTTAGATGATAGTGCCCTGCATGTAAATGGCGAGAGCTTGGAGATGGACTCAGAAGATGAAGAC
  TCCGATGAGCTGGAGGATGACGAGGACCATGGAGCTGAGCAAGCGGCTGCATTTCCCACCGAGGATAGTA
  GGACTTCTAAAGAGAGCATGTCTGAGACTGACCGGGCTGCAAAGATGGATGGAGATTCAGAGGAGGAACA
  GGAGTCTCCCGACACAGGGGAGGATGAAGATGGTGGAGACGAGTCTGACCTGAGTTCTGAATCCAGTATC
  AAGAAGAAATTTCTCAAGAGGAGAGGGAAGACTGACAGCCCCTGGATCAAACCTGCTCGGAAAAGGAGGC
  GGAGAAGTAGAAAGAAGCCGAGCAGCATGCTTGGTTCCGAGGCTTGTAAGTCATCTCCAGGAAGCATGGA
  GCAGGCAGCTCTGGGAGACAGCGCTGGCTATATGGAAGTTTCCCTGGATTCCCTGGATCTCCGTGTCAGA
  GGAATTCTGTCTTCCCAGACAGAAAATGAAGGGCTGGCCAGTGGTCCGGATGTGCTGGGGACGGATGGCC
  TCCAGGAAGTGCCTCTCTGCAGCTGCCGAATGGAAACCCCCAAGAGCCGCGAGATCAGCACCCTGGCCAA
  CAACCAGTGCATGGCCACTGAGAGCGTGGATCACGAAGGTAACTTCATGGAATGCCAGCCTGAGAGCAGC
  ATCTCTCATCGTTTCCATAAGGACTGTGCCTCTCGAGTCAACAATGCCAGCTACTGTCCCCATTGTGGGG
  AAGAAGCTTCCAAGGCCAAAGAGGTGACCATAGCAAAAGCAGACACAACTTCCACAGTGACCCTAGCCCC
  TGGACAGGAGAAGAGCCTGGCTGCTGAAGGCAGGGCTGACACGACCACGGGCAGCATTGCTGGAGCCCCA
  GAGGATGAAAGATCGCAGAGCACAGCCCCCCAGGCACCAGAGTGCTTCGACCCTGCCGGACCGGCTGGGC
  TCGTGAGGCCGACATCTGGCCTTTCCCAGGGCCCAGGAAAGGAAACCTTGGAAAGTGCTCTAATCGCTCT
  AGACTCTGAAAAACCCAAGAAACTTCGCTTCCACCCAAAGCAGCTGTACTTCTCTGCCAGGCAGGGTGAG
  CTGCAGAAGGTGCTTCTCATGCTGGTTGATGGAATTGATCCCAACTTCAAAATGGAGCACCAAAGTAAGC
  GTTCCCCATTACATGCTGCTGCGGAGGCTGGCCACGTGGACATCTGCCACATGCTGGTTCAGGCGGGTGC
  CAATATTGACACTTGCTCAGAGGACCAGCGGACCCCACTGATGGAGGCTGCAGAGAACAACCACTTGGAT
  GCAGTGAAGTACCTCATCAAGGCTGGAGCACAGGTGGATCCGAAGGACGCAGAGGGCTCCACATGTTTGC
  ATTTGGCTGCCAAGAAAGGCCACTATGATGTGGTTCAGTATCTGCTTTCAAATGGACAGATGGATGTCAA
  CTGCCAGGATGACGGTGGATGGACACCTATGATCTGGGCCACTGAGTACAAGCACGTGGAGCTGGTGAAG
  CTGCTGCTGTCTAAGGGCTCTGACATCAACATCCGGGACAACGAGGAGAACATTTGTCTGCACTGGGCAG
  CATTTTCAGGCTGTGTGGACATAGCTGAAATACTTCTGGCTGCCAAGTGTGACCTGCATGCTGTGAATAT
  CCATGGAGACTCACCCCTGCACATCGCAGCCAGGGAGAATCGCTACGACTGTGTTGTCCTCTTTCTTTCT
  CGGGATTCAGATGTTACTCTGAAAAACAAGGAAGGAGAGACTCCCTTGCAGTGTGCAAGTCTCAGTTCGC
  AGGTGTGGAGTGCATTGCAGATGAGCAAAGCACTTCGGGACTCAGCCCCTGACAAGCCCGTTGCTGTTGA
  GAAGACGGTGAGCAGGGATATCGCTCGAGGGTATGAGCGCATTCCCATTCCCTGTGTCAATGCTGTGGAC
  AGTGAGCTGTGTCCTACCAACTATAAGTATGTCTCCCAGAACTGTGTGACATCCCCCATGAACATTGACA
  GGAACATCACTCATTTGCAGTACTGCGTGTGTGTAGATGACTGCTCCTCTAGCACCTGCATGTGTGGCCA
  GCTGAGCATGCGCTGCTGGTATGATAAGGATGGCCGACTTCTGCCTGAGTTTAACATGGCAGAACCACCC
  TTGATCTTCGAGTGCAATCATGCCTGCTCATGCTGGAGGAACTGCCGCAATCGTGTGGTGCAAAATGGTC
  TCAGGGCAAGGCTGCAGCTTTATCGGACACAGGACATGGGCTGGGGTGTGCGGTCCCTCCAGGATATCCC
  ACTGGGCACCTTTGTCTGCGAATACGTAGGGGAGCTGATTTCGGACTCTGAAGCTGATGTTCGGGAAGAG
  GACTCTTACCTCTTTGATCTTGACAATAAGGATGGAGAGGTATACTGCATTGACGCTCGGTTCTATGGGA
  ATGTCAGCCGGTTCATAAACCACCACTGCGAACCCAACCTTGTGCCTGTGCGAGTGTTCATGTCACACCA
  GGACCTGCGGTTTCCCAGGATTGCCTTCTTCAGTACCCGCCTGATTCAGGCTGGGGAGCAGCTCGGGTTC
  GACTACGGGGAGCGCTTTTGGGACGTCAAGGGCAAGCTCTTCAGTTGCCGGTGTGGGTCTTCCAAGTGTC
  GGCACTCAAGCGCAGCCCTGGCCCAGAGGCAAGCCAGTGCAGCCCAGGAGCCTCAGGAGAATGGCCTTCC
  AGATACCAGCTCTGCAGCCGCTGCTGACCCCCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226605 representing NM_001109687
  Red=Cloning site Green=Tags(s)
  
  MAAADAEQAVLAKQETKQDCCMKTELLREDTPMAADEGSTEKQEGETPMAADGETNGSCEKSGDPSHLNA
  PKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRT
  PNILTSSLPGHAAKTLPGGASKCRTLSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPK
  SILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLA
  AAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKALDDSALHVNGESLEMDSEDED
  SDELEDDEDHGAEQAAAFPTEDSRTSKESMSETDRAAKMDGDSEEEQESPDTGEDEDGGDESDLSSESSI
  KKKFLKRRGKTDSPWIKPARKRRRRSRKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVR
  GILSSQTENEGLASGPDVLGTDGLQEVPLCSCRMETPKSREISTLANNQCMATESVDHEGNFMECQPESS
  ISHRFHKDCASRVNNASYCPHCGEEASKAKEVTIAKADTTSTVTLAPGQEKSLAAEGRADTTTGSIAGAP
  EDERSQSTAPQAPECFDPAGPAGLVRPTSGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGE
  LQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLD
  AVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVELVK
  LLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLS
  RDSDVTLKNKEGETPLQCASLSSQVWSALQMSKALRDSAPDKPVAVEKTVSRDIARGYERIPIPCVNAVD
  SELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQLSMRCWYDKDGRLLPEFNMAEPP
  LIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPLGTFVCEYVGELISDSEADVREE
  DSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQDLRFPRIAFFSTRLIQAGEQLGF
  DYGERFWDVKGKLFSCRCGSSKCRHSSAALAQRQASAAQEPQENGLPDTSSAAAADPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109687
• ORF Size: 3744 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001109687.1, NM_001109687.2, NP_001103157.1
• RefSeq Size: 4947
• RefSeq ORF: 3747
• Locus ID: 77683
• MW: 137.1 kDa