Txnrd1 (NM_001042523) Mouse Tagged ORF Clone

CAT#: MR226607

Sepn1 (Myc-DDK-tagged) - Mouse selenoprotein N, 1 (Sepn1), (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Symbol: Txnrd1
• Synonyms: TR; TR1; TrxR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226607 representing NM_001042523
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGTTGATGACTGCTGGCTGTACTTCCCAGCTTCTCGAGGTAGAACCTTTGTGCAGACTGTCTGGG
  TGGCACCCACTTGCCCCAACTGTTGCTGGTTTCCAGGTTTTCTCCCTCCAGTCCCCCGGCCACCACATGT
  GCCCCGTGTGCTGCTGAGGGGCCCTCGTGGGGCTGTGCTTCCTGCTTCACGTCCCTCCAAGACACTCCCC
  TCCTCATCCCAGACGCCCTGTCCTACTGACCCCTGTATCTGCCCTCCACCCTCCACACCTGATAGTAGGC
  AGGAAAAAAATACGCAATCTGAGCTGCCGAACAAAAAAGGCCAACTTCAAAAGCTGCCAACAATGAATGG
  CTCCAAAGATCCCCCTGGGTCCTATGACTTCGACCTGATCATCATTGGAGGAGGCTCAGGAGGACTGGCA
  GCAGCTAAGGAGGCAGCCAAATTTGACAAGAAAGTGCTGGTCTTGGATTTTGTCACACCGACTCCTCTTG
  GGACCAGATGGGGTCTCGGAGGAACGTGTGTGAATGTGGGTTGCATACCTAAGAAGCTGATGCACCAGGC
  AGCTTTGCTCGGACAAGCTCTGAAAGACTCGCGCAACTATGGCTGGAAAGTCGAAGACACAGTGAAGCAT
  GACTGGGAGAAAATGACGGAATCTGTGCAGAGTCACATCGGCTCGCTGAACTGGGGCTACCGCGTAGCTC
  TCCGGGAGAAAAAGGTCGTCTATGAGAATGCTTACGGGAGGTTCATTGGTCCTCACAGGATTGTGGCGAC
  AAATAACAAAGGTAAAGAAAAAATCTATTCAGCAGAGCGGTTCCTCATCGCCACAGGTGAGAGGCCCCGC
  TACCTGGGCATCCCTGGAGACAAAGAGTACTGCATCAGCAGTGATGATCTTTTCTCCTTGCCTTACTGCC
  CGGGGAAGACCCTAGTAGTTGGTGCATCCTATGTCGCCTTGGAATGTGCAGGATTTCTGGCTGGTATCGG
  CTTAGACGTCACTGTAATGGTGCGGTCCATTCTCCTTAGAGGATTTGACCAAGACATGGCCAACAAAATC
  GGTGAACACATGGAAGAACATGGTATCAAGTTTATAAGGCAGTTCGTCCCAACGAAAATTGAACAGATCG
  AAGCAGGAACACCAGGCCGACTCAGGGTGACTGCTCAATCCACAAACAGCGAGGAGACCATAGAGGGCGA
  ATTTAACACAGTGTTGCTGGCGGTAGGAAGAGATTCTTGTACGAGAACTATTGGCTTAGAGACCGTGGGC
  GTGAAGATAAACGAAAAAACCGGAAAGATACCCGTCACGGATGAAGAGCAGACCAATGTGCCTTACATCT
  ACGCCATCGGTGACATCCTGGAGGGGAAGCTAGAGCTGACTCCCGTAGCCATCCAGGCGGGGAGATTGCT
  GGCTCAGAGGCTGTATGGAGGCTCCAATGTCAAATGTGACTATGACAATGTCCCAACGACTGTATTTACT
  CCTTTGGAATATGGCTGTTGTGGCCTCTCTGAAGAAAAAGCCGTAGAGAAATTTGGGGAAGAAAATATTG
  AAGTTTACCATAGTTTCTTTTGGCCATTGGAATGGACAGTCCCATCCCGGGATAACAACAAATGTTATGC
  AAAAATAATCTGCAACCTTAAAGACGATGAACGTGTCGTGGGCTTCCACGTGCTGGGTCCAAACGCTGGA
  GAGGTGACGCAGGGCTTTGCGGCTGCGCTCAAGTGTGGGCTGACTAAGCAGCAGCTGGACAGCACCATCG
  GCATCCACCCGGTCTGTGCAGAGATATTCACAACGTTGTCAGTGACGAAGCGCTCTGGGGGAGACATCCT
  CCAGTCTGGCTGCTGAGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226607 representing NM_001042523
  Red=Cloning site Green=Tags(s)
  
  MPVDDCWLYFPASRGRTFVQTVWVAPTCPNCCWFPGFLPPVPRPPHVPRVLLRGPRGAVLPASRPSKTLP
  SSSQTPCPTDPCICPPPSTPDSRQEKNTQSELPNKKGQLQKLPTMNGSKDPPGSYDFDLIIIGGGSGGLA
  AAKEAAKFDKKVLVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALKDSRNYGWKVEDTVKH
  DWEKMTESVQSHIGSLNWGYRVALREKKVVYENAYGRFIGPHRIVATNNKGKEKIYSAERFLIATGERPR
  YLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKI
  GEHMEEHGIKFIRQFVPTKIEQIEAGTPGRLRVTAQSTNSEETIEGEFNTVLLAVGRDSCTRTIGLETVG
  VKINEKTGKIPVTDEEQTNVPYIYAIGDILEGKLELTPVAIQAGRLLAQRLYGGSNVKCDYDNVPTTVFT
  PLEYGCCGLSEEKAVEKFGEENIEVYHSFFWPLEWTVPSRDNNKCYAKIICNLKDDERVVGFHVLGPNAG
  EVTQGFAAALKCGLTKQQLDSTIGIHPVCAEIFTTLSVTKRSGGDILQSGC*G
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042523
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.

Reference Data

• RefSeq: NM_001042523.1, NP_001035988.1
• RefSeq Size: 3634
• RefSeq ORF: 1842
• Locus ID: 50493
• Gene Summary: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms. [provided by RefSeq, May 2017]